Anti-nSMase3 (C-terminal region) Antibody

Name: Anti-nSMase3 (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: SP0281
Price: 329.0 USD
Availability: InStock

2 Citations

Our nSMase3 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat nSMase3 (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC, IP.

Immunocytochemical labeling of nSMase3 in aldehyde-fixed and NP-40-permeabilized mouse C2C12 cells. The cells were labeled with rabbit polyclonal anti-nSMase3 (SP0281) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

Western blot of nSMase3a and nSMase3b GFP transfectants (A) and nSMase3 siRNA knockdown in C2C12 myotubes (B). Both blots were probed with anti-nSMase3 (C-terminal region) rabbit polyclonal antibody (SP0281). (Image provided by Dr. Jennifer Moylan, Dept. Physiology, University of Kentucky).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IP, WB

Host Species

Rabbit

Go to Full Product Details

SKU: SP0281

Product Details

Target

nSMase3 (C-terminal region)

Target Description

Cellular stress leads to hydrolysis of sphingomyelin to generate lipid second messenger molecules including ceramide, sphingosine, and sphingosine-1-phosphate. A variety of sphingomyelinase activities have been described that differ in tissue and subcellular distribution, as well as pH and cation dependence. These enzymes generate ceramide for specific signaling pathways that lead to a wide range of cellular responses, such as apoptosis, cell cycle arrest, cell survival, and cell proliferation. Neutral sphingomyelinases (nSMases) are Mg2+-dependent neutral pH SMases, and the family includes nSMase1, nSMase2, and nSMase3. These nSMases differ in their tissue distribution and subcellular localization. nSMase3 (also known as SMPD4) is highly expressed in skeletal muscle and heart tissues, and is expressed in many cancer cell lines. The nSMase3 protein is localized to endoplasmic reticulum and Golgi, and may be upregulated in response to DNA damage and cell stress.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IP, WB

Host Species

Rabbit

Gene Name

SMPD4

Molecular Weight

85-90

Antigen

nSMase3 synthetic peptide corresponds to amino acids in the C-terminal region of mouse nSMase3. This sequence is highly conserved in rat and human nSMase3, and has low homology to other nSMase family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:250
IP: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects an 85-90 kDa* band corresponding to nSMase3 on SDS-PAGE immunoblots of adult mouse heart and mouse C2C12 cells. The antibody works for western blot, ELISA, immunoprecipitation, and immunocytochemistry.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

SMPD4,neutral sphingomyelinase 3, nSMase sphingomyelin

UniProt Details

UniProt (Human): Q9NXE4
UniProt (Immunogen Species): Q6ZPR5

Product Specific References for Applications and Species

Immunocytochemistry: Human
Western Blot: Human

Immunocytochemistry: Human
PMID	Dilution	Publication
31495489	1:500	Magini, P, et al. 2019. Loss of SMPD4 Causes a Developmental Disorder Characterized by Microcephaly and Congenital Arthrogryposis. American journal of human genetics, 689-705.

Western Blot: Human
PMID	Dilution	Publication
31495489	1:1000	Magini, P, et al. 2019. Loss of SMPD4 Causes a Developmental Disorder Characterized by Microcephaly and Congenital Arthrogryposis. American journal of human genetics, 689-705.

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Anti-nSMase3 (C-terminal region) Antibody

SKU: SP0281

Product Details

Product Specific References for Applications and Species

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