Name: Anti-NSF (N-ethylmaleimide Sensitive Fusion Protein) Antibody
Brand: PhosphoSolutions
SKU: 1525-NSF
Price: 415.0 USD
Availability: InStock

Our Anti-NSF (N-ethylmaleimide sensitive fusion protein) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat NSF (N-ethylmaleimide sensitive fusion protein) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Rat

Expected Reactivity

Human, Non-Human Primate

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

NSF

Molecular Weight

75 kDa

Go to Full Product Details

Western blot of rat striatal lysate showing specific labeling of the ~75 kDa NSF protein.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-NSF (N-ethylmaleimide Sensitive Fusion Protein) Antibody

Product Details

Target

NSF (N-ethylmaleimide sensitive fusion protein)

Target Description

NSF (N-ethylmaleimide sensitive fusion protein) is a critical component of the SNARE (soluble NSF attachment protein receptors) protein complex that is involved in synaptic vesicle trafficking. Specifically, NSF has been found to be essential in membrane fusion. Furthermore, NSF has been recently demonstrated to bind other protein complexes such as AMPA receptor subunits (GluR2), GATE-16, LMA-1 and Rabs suggesting a more diverse role in the assembly of various protein complexes (Whiteheart et al., 2004).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

NSF

Molecular Weight

75 kDa

Antigen

Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat NSF, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Rat

Expected Reactivity

Human, Non-Human Primate

Antibody Registry ID

AB_2492191

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via chromatography on an affinity column made with the C-terminal peptide used as antigen.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~75 kDa NSF protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

N ethylmaleimide sensitive factor antibody, N ethylmaleimide sensitive factor like protein antibody, N ethylmaleimide sensitive fusion protein antibody, N-ethylmaleimide-sensitive fusion protein antibody, NEM sensitive fusion protein antibody, NEM-sensitive fusion protein antibody, NSF antibody, NSF_HUMAN antibody, SKD 2 antibody, SKD2 antibody, Vesicle fusing ATPase antibody, Vesicle-fusing ATPase antibody, Vesicular fusion protein NSF antibody, Vesicular-fusion protein NSF antibody

UniProt Details

UniProt (Human): P46459
UniProt (Immunogen Species): Q9QUL6

NCBI Gene ID

60355

Product Specific Protocols

Western Blot Protocol: Download

General References

Schweizer, F.E., Dresbach, T., DeBello, W.M., O'Connor, V., Augustine, G.J. and Betz, H., 1998. Regulation of neurotransmitter release kinetics by NSF. Science, 279(5354), pp.1203-1206. PMID: 9469810
Matveeva, E.A., Whiteheart, S.W., Vanaman, T.C. and Slevin, J.T., 2001. Phosphorylation of the N-ethylmaleimide-sensitive factor is associated with depolarization-dependent neurotransmitter release from synaptosomes. Journal of Biological Chemistry, 276(15), pp.12174-12181. PMID: 11278345
Whiteheart, S.W. and Matveeva, E.A., 2004. Multiple binding proteins suggest diverse functions for the N-ethylmaleimide sensitive factor. Journal of structural biology, 146(1-2), pp.32-43. PMID: 15037235

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Anti-NSF (N-ethylmaleimide Sensitive Fusion Protein) Antibody

Our Anti-NSF (N-ethylmaleimide sensitive fusion protein) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat NSF (N-ethylmaleimide sensitive fusion protein) and is antigen affinity purified from pooled serum. It is great for use in WB.