Name: Anti-NMDA NR2B Subunit, N-terminus Antibody
Brand: PhosphoSolutions
SKU: 1503-NR2B
Price: 415.0 USD
Availability: InStock

Our Anti-NMDA NR2B Subunit, N-terminus rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat NMDA NR2B Subunit, N-terminus and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate

Applications

ICC, IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

GRIN2B

Molecular Weight

180 kDa

Go to Full Product Details

Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.

Immunostaining of 14 DIV rat cortical neurons showing NR2B (green, 1:400) and PSD95(red). Photo courtesy of Gang Liu.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-NMDA NR2B Subunit, N-terminus Antibody

Product Details

Target

NMDA NR2B Subunit, N-terminus

Target Description

The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned and it can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Overexpression of the NR2B-subunit of the NMDA receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

GRIN2B

Molecular Weight

180 kDa

Antigen

Synthetic peptide corresponding to amino acid residues from the N-terminal region of the NR2B subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate

Antibody Registry ID

AB_2492178

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:500
ICC: 1:400

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~180 kDa NR2B subunit of the NMDA receptor.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

EPND antibody, FESD antibody, GluN2A antibody, Glutamate [NMDA] receptor subunit epsilon-1 antibody, Glutamate receptor antibody, Glutamate receptor ionotropic N methyl D aspartate 2A antibody, GRIN 2A antibody, GRIN2A antibody, hNR2A antibody, LKS antibody, N methyl D aspartate receptor channel subunit epsilon 1 antibody, N Methyl D Aspartate Receptor Subtype 2A antibody, N methyl D aspartate receptor subunit 2A antibody, N-methyl D-aspartate receptor subtype 2A antibody, NMDA receptor subtype 2A antibody, NMDAR 2A antibody, NMDAR2A antibody, NMDE1_HUMAN antibody, NR2A antibody, OTTHUMP00000160135 antibody, OTTHUMP00000174531 antibody

UniProt Details

UniProt (Human): Q13224
UniProt (Immunogen Species): Q00960

NCBI Gene ID

24410

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Immunohistochemistry: Rat
Western Blot: Mouse

Immunocytochemistry: Rat
PMID	Dilution	Publication
37270156	1:300	Nuwer, JL, et al. 2023. Long-term a5 GABA A Receptor Negative Allosteric Modulator Treatment Reduces NMDAR-mediated Neuronal Excitation and Maintains Basal Neuronal Inhibition. Neuropharmacology, 109587.

Immunohistochemistry: Rat
PMID	Dilution	Publication
29339324	1:500	Aroniadrou-Anderjaska, V., et al. 2018. Oscillatory synchronous inhibition in the basolateral amygdala and its primary dependence on NR2A-containing NMDA receptors. Neuroscience, 373, pp.145-158.

Western Blot: Mouse
PMID	Dilution	Publication
31235881	1:1000	Pallas-Bazarra, N., et al. 2019. Tau is required for the function of extrasynaptic NMDA receptors. Scientific Reports, 9(1), p.9116.

Product Specific Protocols

NR2B Free Floating IHF Protocol: Download

NR2B ICC Protocol: Download

Western Blot Protocol: Download

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Anti-NMDA NR2B Subunit, N-terminus Antibody

Our Anti-NMDA NR2B Subunit, N-terminus rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat NMDA NR2B Subunit, N-terminus and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.