Name: Anti-NMDA NR2A Subunit (Tyr1325) Antibody
Brand: PhosphoSolutions
SKU: p1514-1325
Price: 415.0 USD
Availability: InStock

Our Anti-NMDA NR2A Subunit (Tyr1325) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat NMDA NR2A Subunit (Tyr1325) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Hamster, Sheep

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

GRIN2A

Molecular Weight

180 kDa

Go to Full Product Details

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDAR phosphorylated at Tyr1325 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-NMDA NR2A Subunit (Tyr1325) Antibody

Product Details

Target

NMDA NR2A Subunit (Tyr1325)

Target Description

The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Recently, phosphorylation of Tyrosine 1325 of the NR2A subunit has been shown to be increased in human brain tissue sections from HIV-infected individuals with encephalitis (King et al., 2010). In addition, Tyr-1325 phosphorylation has been linked with depression-related behavior (Taniguchi et al., 2009).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

GRIN2A

Molecular Weight

180 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1325 of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Hamster, Sheep

Antibody Registry ID

AB_2492174

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~180k NMDAR NR2A-subunit protein phosphorylated at Tyr1325 in Western blots. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr1325

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

EPND antibody, FESD antibody, GluN2A antibody, Glutamate [NMDA] receptor subunit epsilon-1 antibody, Glutamate receptor antibody, Glutamate receptor ionotropic N methyl D aspartate 2A antibody, GRIN 2A antibody, GRIN2A antibody, hNR2A antibody, LKS antibody, N methyl D aspartate receptor channel subunit epsilon 1 antibody, N Methyl D Aspartate Receptor Subtype 2A antibody, N methyl D aspartate receptor subunit 2A antibody, N-methyl D-aspartate receptor subtype 2A antibody, NMDA receptor subtype 2A antibody, NMDAR 2A antibody, NMDAR2A antibody, NMDE1_HUMAN antibody, NR2A antibody, OTTHUMP00000160135 antibody, OTTHUMP00000174531 antibody

UniProt Details

UniProt (Immunogen Species): Q00959

NCBI Gene ID

24409

Product Specific References for Applications and Species

Western Blot: Human | Mouse | Rat

Western Blot: Human
PMID	Dilution	Publication
31135041	1:1000	Dedek, A., et al. 2019. Loss of STEP61 couples disinhibition to N-methyl-d-aspartate receptor potentiation in rodent and human spinal pain processing. Brain, 142(6), pp.1535-1546.

Western Blot: Mouse
PMID	Dilution	Publication
34433031	not listed	Shi, X., et al. 2021. Disrupting phosphorylation of Tyr-1070 at GluN2B selectively produces resilience to depression-like behaviors. Cell Reports, 36(8), p.109612.
34044147	1:1000	Zhang, L., et al. 2021. Tyrosine phosphatase PTP1B impairs presynaptic NMDA receptor-mediated plasticity in a mouse model of Alzheimer's disease. Neurobiology of Disease, 156, p.105402.

Western blot: Rat
PMID	Dilution	Publication
33333103	1:200	Avchalumov, Y., et al. 2020. Chronic ethanol exposure differentially alters neuronal function in the medial prefrontal cortex and dentate gyrus. Neuropharmacology, Dec. 15, p.108438.
31835746	1:200	Somkuwar, S.S., et al. 2019. Individual Differences in Ethanol Drinking and Seeking Behaviors in Rats Exposed to Chronic Intermittent Ethanol Vapor Exposure is Associated with Altered CaMKII Autophosphorylation in the Nucleus Accumbens Shell. Brain Sciences, 9(12), p.367.
31135041	1:1000	Dedek, A., et al. 2019. Loss of STEP61 couples disinhibition to N-methyl-d-aspartate receptor potentiation in rodent and human spinal pain processing. Brain, 142(6), pp.1535-1546.
29441405	1:200	Takashima, Y., et al. 2018. Neuroadaptations in the dentate gyrus following contextual cued reinstatement of methamphetamine seeking. Brain Structure and Function, pp.1-15.
26220171	1:200	Staples, M.C., et al. 2015. Developmental effects of wheel running on hippocampal glutamate receptor expression in young and mature adult rats. Neuroscience. Jul 25. pii: S0306-4522(15)00672-7.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-NMDA NR2A Subunit (Tyr1325) Antibody

Our Anti-NMDA NR2A Subunit (Tyr1325) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat NMDA NR2A Subunit (Tyr1325) and is antigen affinity purified from pooled serum. It is great for use in WB.