Name: Anti-NMDA NR2B Subunit Antibody
Brand: PhosphoSolutions
SKU: 1499-NR2B
Price: 415.0 USD
Availability: InStock

Our Anti-NMDA NR2B Subunit rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat NMDA NR2B Subunit and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, IP.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

IHC, IP, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

GRIN2B

Molecular Weight

180 kDa

Go to Full Product Details

Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.

Size: 10 µg

Variant

Price:

$415

Quantity:

Bulk Order Anti-NMDA NR2B Subunit Antibody

Product Details

Target

NMDA NR2B Subunit

Target Description

The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned and it can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Overexpression of the NR2B-subunit of the NMDA receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, IP, WB

Host Species

Rabbit

Gene Name

GRIN2B

Molecular Weight

180 kDa

Antigen

Fusion protein from the C-terminal region of the NR2B subunit of the rat NMDA receptor.

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2492175

Storage

Reconstitute in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Lyophilized

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Buffer

Lyophilized

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:1000
IP: 3 µl per 200 µg lysate

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~180 kDa NR2B subunit of the NMDA receptor. Immunolabeling is blocked by preadsorption of antibody with the fusion protein used to generate the antibody. No reactivity towards the NR2A and NR2C subunits.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

AW490526 antibody, EIEE27 antibody, Glutamate [NMDA] receptor subunit epsilon 2 antibody, Glutamate [NMDA] receptor subunit epsilon-2 antibody, Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody, Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody, Glutamate receptor ionotropic NMDA2B antibody, Glutamate receptor subunit epsilon 2 antibody, Glutamate receptor ionotropic NMDA2B (epsilon 2) antibody, GRIN 2B antibody, GRIN2B antibody, hNR 3 antibody, hNR3 antibody, MGC142178 antibody, MGC142180 antibody, MRD6 antibody, N methyl D asparate receptor channel subunit epsilon 2 antibody, N methyl D aspartate receptor subtype 2B antibody, N methyl D aspartate receptor subunit 2B antibody, N methyl D aspartate receptor subunit 3 antibody, N-methyl D-aspartate receptor subtype 2B antibody, N-methyl-D-aspartate receptor subunit 3 antibody, NMDA NR2B antibody, NMDA R2B antibody, NMDAR2B antibody, NMDE2 antibody, NMDE2_HUMAN antibody, NME2 antibody, NR2B antibody, NR3 antibody

UniProt Details

UniProt (Human): Q13224
UniProt (Immunogen Species): Q00960

NCBI Gene ID

24410

Product Specific References for Applications and Species

Immunoprecipitation: Mouse | Rat
Western Blot: Mouse | Rat

Immunoprecipitation: Mouse
PMID	Dilution	Publication
17673549	not listed	Chernova, T., et al. 2007. Neurite Degeneration Induced by Heme Deficiency Mediated via Inhibition of NMDA Receptor-Dependent Extracellular Signal-Regulated Kinase 1/2 Activation. J. Neurosci., 27: 8475 - 8485.

Immunoprecipitation: Rat
PMID	Dilution	Publication
12536146	3ul per 200ug lysate	Alvestad, R.M., et al. 2003. Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025.

Western Blot: Mouse
PMID	Dilution	Publication
25971971	not listed	Tassin, T.C., et al. 2015. Regulation of ERK kinase by MEK1 kinase inhibition in the brain. Journal of Biological Chemistry, 290(26), pp.16319-16329.
23968838	1:2500	Bostrom, C.A., et al. 2013. Rescue of NMDAR-dependent synaptic plasticity in Fmr1 knock-out mice. Cerebral cortex, bht237.
23325244	1:500-1:1000	Brady, M.L., et al. 2013. Moderate prenatal alcohol exposure reduces plasticity and alters NMDA receptor subunit composition in the dentate gyrus. Journal of Neuroscience, 33(3), pp.1062-1067.
21464302	1:1000	Hicklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. Proc Natl Acad Sci USA 108(16):6650-5.
17687025	not listed	Chernova, T., et al. 2007. Neurite Degeneration Induced by Heme Deficiency Mediated via Inhibition of NMDA Receptor-Dependent Extracellular Signal-Regulated Kinase 1/2 Activation. J. Neurosci., 27: 8475 - 8485.
17673549	not listed	Norris, E.H., et al. 2007. Modulation of NR2B-regulated contextual fear in the hippocampus by the tissue plasminogen activator system. PNAS, 104: 13473 – 1347.

Western Blot: Rat
PMID	Dilution	Publication
26706598	not listed	O’Leary, H., et al. 2016. Enhanced long term potentiation and decreased AMPA receptor desensitization in the acute period following a single kainate induced early life seizure. Neurobiology of disease, 87, 134-144.
21464302	1:1000	Hicklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. Proc Natl Acad Sci USA 108(16):6650-5.
18819923	1:1000	Davies, K.D., et al. 2008. Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not -Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization. J. Biol. Chem., 283: 33138 - 33146.
12536146	1:1000	Alvestad, R.M., et al. 2003. Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025.

Product Specific Protocols

Western Blot Protocol: Download

Recover password

Create my account

Anti-NMDA NR2B Subunit Antibody

Our Anti-NMDA NR2B Subunit rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat NMDA NR2B Subunit and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, IP.