Our Anti-NMDA NR1 Subunit, Splice Variant N1 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat NMDA NR1 Subunit, Splice Variant N1 and is antigen affinity purified. It is great for use in WB, IHC.
Western blot of 10 µg of HEK 293 cells showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor containing the N1 splice variant insert (lane 1). 1) NR1 subunit containing the N1 and C2' Insert; 2) NR1 subunit containing only the C2 Insert; 3) NR1 subunit containing the C1 and C2' Insert; 4) without NR1 expression.
The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
IHC, WB
Rabbit
GRIN1
120 kDa
Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, N1 splice variant insert of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).
Reconstitute in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Lyophilized
Prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.
Lyophilized
WB: 1:1000
WB Brain: 1:1000
IHC: 1:1000 - 1:2000
Unconjugated
Specific for endogenous levels of the ~120 kDa NR1 subunit, N1 splice variant insert protein of the NMDA receptor. Does not recognize NR1 subunits of the NMDA receptor that do not contain the N1 insert.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Brady, M.L., et al. 2013. Moderate prenatal alcohol exposure reduces plasticity and alters NMDA receptor subunit composition in the dentate gyrus. Journal of Neuroscience. 33(3):1062-7.
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