Name: Anti-NMDA NR1 Subunit, Splice Variant C2 Antibody
Brand: PhosphoSolutions
SKU: 1506-C2
Price: 415.0 USD
Availability: InStock

Our Anti-NMDA NR1 Subunit, Splice Variant C2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat NMDA NR1 Subunit, Splice Variant C2 and is antigen affinity purified. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Human

Applications

IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

GRIN1

Molecular Weight

120 kDa

Go to Full Product Details

Western blot of 10 µg of HEK 293 cells showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor containing the C2 splice variant insert (lane 2). 1) HEK cells without NR1 expression; 2) NR1 subunit containing only the C2 Insert; 3) NR1 subunit containing the C1 and C2' insert; 4) NR1 subunit containing the N1 and C2' insert.

Size: 25 µg

Variant

Price:

$415

Quantity:

Bulk Order Anti-NMDA NR1 Subunit, Splice Variant C2 Antibody

Product Details

Target

NMDA NR1 Subunit, Splice Variant C2

Target Description

The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1 subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1 subunit generates up to eight NR1 subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

GRIN1

Molecular Weight

120 kDa

Antigen

Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, C2 splice variant insert of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Human

Antibody Registry ID

AB_2492167

Storage

Reconstitute in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Lyophilized

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Buffer

Lyophilized

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:1000 - 1:2000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~120 kDa NR1 subunit of the NMDA Receptor containing the C2 splice variant insert. Does not recognize the NR1 subunits of the NMDA receptor that do not contain the C2 insert.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

GluN1 antibody, Glutamate [NMDA] receptor subunit zeta-1 antibody, Glutamate receptor ionotropic N methyl D aspartate 1 antibody, Glutamate receptor ionotropic N-methyl-D aspartate subunit 1 antibody, glutamate receptor ionotropic NMDA 1 antibody, Grin1 antibody, MRD8 antibody, N methyl D aspartate receptor antibody, N methyl D aspartate receptor channel subunit zeta 1 antibody, N methyl D aspartate receptor subunit NR1 antibody, N-methyl-D-aspartate receptor subunit NR1 antibody, NMD-R1 antibody, NMDA 1 antibody, NMDA R1 antibody, NMDAR1 antibody, NMDA receptor 1 antibody, NMDA1 antibody, NMDAR antibody, NMDZ1_HUMAN antibody, NR1 antibody

UniProt Details

UniProt (Human): Q05586
UniProt (Immunogen Species): P35439

NCBI Gene ID

24408

Product Specific References for Applications and Species

Western Blot: Mouse | Rat

Western Blot: Mouse
PMID	Dilution	Publication
23325244	1:500	Brady, M.L., et al. 2013. Moderate prenatal alcohol exposure reduces plasticity and alters NMDA receptor subunit composition in the dentate gyrus. J Neurosci. 33(3):1062-7.
17409241	not listed	Fan, M.M., et al. 2007. Altered NMDA Receptor Trafficking in a Yeast Artificial Chromosome Transgenic Mouse Model of Huntington's Disease. J. Neurosci., 27: 3768 - 3779.

Western Blot: Rat
PMID	Dilution	Publication
11740502	not listed	Grosshands, D.R., et al. 2002. LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nature neuroscience, 5(1), p.27.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-NMDA NR1 Subunit, Splice Variant C2 Antibody

Our Anti-NMDA NR1 Subunit, Splice Variant C2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat NMDA NR1 Subunit, Splice Variant C2 and is antigen affinity purified. It is great for use in WB, IHC.