Anti-NLRC4 Antibody

Name: Anti-NLRC4 Antibody
Brand: PhosphoSolutions
SKU: NP5381
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

NLRC4

Molecular Weight

110

Go to Full Product Details

Western blot image of mouse recombinant NLRC4 (Ser-533) phosphorylated peptide (lanes 1 & 4) and dephosphorylated peptide (lanes 2 & 5), as well as human PMA-differentiated THP1 cells (lanes 3 & 6). The blots were probed with rabbit polyclonals anti-NLRC4 (a.a. 525-538) (lanes 1-3) and anti-NLRC4 (Ser-533) phospho-specific (lanes 4-6).

Volume: 100 µL

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Product Details

Target

NLRC4

Target Description

The nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family is a diverse family of cytoplasmic innate immune receptors that are involved in recognition of pathogen-associated molecular patterns. NLRs are important for pathogen sensing, transcriptional activation of proinflammatory cytokines and activation of inflammatory caspases. NLRC4 (IPAF, CARD12) forms the inflammasome that responds to bacterial flagellin. This inflammasome is activated by NLRC4 oligomerization, NAIP protein binding, and activation of caspase-1 leading to pyroptosis. NLRC4 is phosphorylated on Ser-533 by PKCδ following infection of macrophages with S. typhimurium. Mutant forms of NLRC4 demonstrate that an unphosphorylatable form (S533A) does not activate caspase-1 and pyroptosis in response to S. typhimurium, while a phosphomimetic NLRC4 (S533D) mutant causes rapid macrophage pyroptosis without infection. Thus, PKCδ phosphorylation of NLRC4 (S533) may be a critical event in inflammasome activation and host innate immunity.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

NLRC4

Molecular Weight

110

Antigen

NLRC4 synthetic peptide (coupled to carrier) corresponding to amino acids in the central region of mouse NLRC4. This sequence has high homology to human and rat NLRC4, and is not homologous to sequences in other NLR family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody antibody detects a 110 kDa* protein on SDS-PAGE immunoblots of PMA-differentiated THP1 human macrophages. In addition, the antibody detects a mouse recombinant phospho-NLRC4 (Ser-533) protein fragment.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

CARD12, CLAN1, IPAF, NLR family CARD domain-containing protein 4, NOD-like receptor 4

UniProt Details

UniProt (Human): Q9NPP4
UniProt (Immunogen Species): Q3UP24

Product Specific Protocols

Western Blot Protocol: Download

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Anti-NLRC4 Antibody