Anti-NG2/CSPG4 Antibody

Name: Anti-NG2/CSPG4 Antibody
Brand: PhosphoSolutions
SKU: 1360-NG2
Price: 415.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Trial Size

Pooled Serum

Our NG2/CSPG4 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects NG2/CSPG4 and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

CSPG4

Molecular Weight

250 kDa

Go to Full Product Details

Western blot of mouse whole brain lysate showing specific immunolabeling of the ~250 kDa CSPG4/NG2 protein.

Immunostaining of a sagittal section of FFPE rat brain showing strong staining of oligodendrocyte precursor cells (OPC) in the hippocampus using our anti-NG2/CSPG4 (DAB, 1:200) and detected with anti-rabbit HRP. The section is counterstained with hematoxylin to identify DNA (blue).

Size: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-NG2/CSPG4 Antibody

Product Details

Target

NG2/CSPG4

Target Description

Neuron-glial antigen 2 (NG2) is a chondroitin sulfate proteoglycan that is encoded by the CSPG4 gene in humans. It is an excellent oligodendrocyte marker. NG2 is a hallmark protein of oligodendrocyte progenitor cells (OPCs) and has been linked with familial schizophrenia (de Vrij, F.M., et al, 2018). NG2 also plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. It represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells (Luo, W., et al, 2006). NG2 localizes at the apical plasma membrane and relocalizes to the lamellipodia of astrocytoma upon PRKCA phosphorylation.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

CSPG4

Molecular Weight

250 kDa

Antigen

Recombinant human NG2/CSPG4 protein

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2927667

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column to which the recombinant protein antigen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:200

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~250 kDa NG2/CSPG4 protein

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

4732461B14Rik antibody, AN2 antibody, AN2 proteoglycan antibody, Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody, Chondroitin sulfate proteoglycan 4 antibody, Chondroitin sulfate proteoglycan NG2 antibody, Cspg4 antibody, Cspg4 chondroitin sulfate proteoglycan 4 antibody, CSPG4_HUMAN antibody, HMW-MAA antibody, HSN tumor-specific antigen antibody, Kiaa4232 antibody, MCSP antibody, MCSPG antibody, MEL-CSPG antibody, Melanoma chondroitin sulfate proteoglycan antibody, Melanoma-associated chondroitin sulfate proteoglycan antibody, MELCSPG antibody, MSK16 antibody, NG2 antibody

UniProt Details

UniProt (Human): Q6UVK1
UniProt (Immunogen Species): Q6UVK1

NCBI Gene ID

1464

Product Specific Protocols

Western Blot Protocol: Download

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Anti-NG2/CSPG4 Antibody

Our NG2/CSPG4 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects NG2/CSPG4 and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.