Anti-NFH (Neurofilament Heavy Chain) Antibody

Name: Anti-NFH (Neurofilament Heavy Chain) Antibody
Brand: Aves Labs
SKU: NFH
Price: 275.0 USD
Availability: InStock

from Aves Labs Datasheet

54 Citations

NFH protein was purified from bovine spinal cords following the method of Delacourte et al. ["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion” (1980). Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFH protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. These antibodies have been found to cross-react with NFH protein from a variety of species, including human, mouse and rat.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Format

IgY Fraction

Gene Name

NEFH KIAA0845 NFH

Molecular Weight

200-220 kDa

Go to Full Product Details

Cortical neuron from a neo-natal mouse brain culture immunocytochemically stained for NF-H immunoreactivity (1:5000 dilution). Secondary antibody (fluorescein-labeled goat anti-chicken IgY, (1:1000 dilution)

Confocal micrographs of DRG cryosections from WT mouse double-labeled with antibodies against caldendrin (green) and NF200 (Cat no. NFH, 1:1000, purple) or peripherin (Cat no. PER, 1:1000, purple). Arrows and arrowheads indicate cells in which caldendrin is or is not, respectively, co-localized with NF200 or peripherin. Results are representative of at least 3 independent experiments. Scale bar, 100 µm. Image from publication CC-BY-4.0. PMID:36788334

Confocal micrographs of caldendrin KO cultures infected with AAV-mRuby (top) or AAV-mRuby-caldendrin (bottom) showing immunolabeling for NF200 (Cat no. NFH, 1:1000, left) and mRuby (right). NF200-positive DRGNs with and without mRuby fluorescence are indicated by arrows and arrowheads, respectively. Scale bar, 100 µm. Image from publication CC-BY-4.0. PMID:36788334

Representative images of sections of a rat G muscle 7, 14, 21, and 60 dpi, immunostained against NFH (cat. NFH, 1:200; green), α-btx (red), and VAChT (blue), respectively, to label axons, neuromuscular junctions, and the synaptic vesicle-filled motor end plate. Larger numbers of reinnervated NMJs were observed with increased time after injury, with almost complete innervation at 60 dpi. The nicotinic receptor field expands after early denervation. Image from publication CC-BY-4.0. PMID: 36759186

Cortical neuron from a neo-natal mouse brain culture immunocytochemically stained for NF-H immunoreactivity (1:5000 dilution). Secondary antibody (fluorescein-labeled goat anti-chicken IgY, (1:1000 dilution)

Volume: 200 µL

Variant

Price:

$275

Quantity:

Bulk Order Anti-NFH (Neurofilament Heavy Chain) Antibody

Product Details

Target

Neurofilament Heavy Chain (NFH)

Target Description

Human Neurofilament Heavy Chain (NFH) is a 115,378 dalton protein (1072 amino acids) that forms part of the neuronal cytoskeleton. NFH is physically associated with 10 nm intermediate filaments and is said to be one of the "neurofilament triplet" proteins, although it probably serves more to stabilize the cytoskeleton rather than as an integral structural component. NFH immunoreactivity is found in neuronal somata, dendrites and axons.

Format

IgY Fraction

Concentration

1 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

NEFH KIAA0845 NFH

Molecular Weight

200-220 kDa

Antigen

Native NF-H purified from bovine spinal cord

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313552

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with NF-H protein purified from adult bovine brains. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in the buffer described above, and then filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preservative

Dilution Ranges

WB: 1:5000-1:10000
IHC: 1:2000-1:5000
ICC: 1:2000-1:5000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:50000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Synonyms

Neurofilament heavy polypeptide (NF-H) (200 kDa neurofilament protein) (Neurofilament triplet H protein)

UniProt Details

UniProt (Immunogen Species): P12036

Product Specific References for Applications and Species

Immunocytochemistry: Mouse | Rat
Immunohistochemistry: Mouse | Rat
Western Blot: Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
35821510	1:2000	Rogers, E.A., et al. 2022. Utilizing novel TBI-on-a-chip device to link physical impacts to neurodegeneration and decipher primary and secondary injury mechanisms. Scientific reports, 11838.
29404402	1:500	Hines, T.J., et al. 2018. An Essential Postdevelopmental Role for Lis1 in Mice. eNeuro, .
28828403	1:1000	Lerch, J.K., et al. 2018. Stress Increases Peripheral Axon Growth and Regeneration through Glucocorticoid Receptor-Dependent Transcriptional Programs. eNeuro, .

Immunocytochemistry: Rat
PMID	Dilution	Publication
30038033	1:500	Lee, S.J., et al. 2018. hnRNPs interacting with mRNA localization motifs define axonal RNA regulons. Molecular & Cellular Proteomics, 2091-2106.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
37607547	1:500	Koutsioumpa, C, et al. 2023. Skin-type-dependent development of murine mechanosensory neurons. Developmental Cell, .
37541195	1:1000	Wolfson, RL, et al. 2023. DRG afferents that mediate physiologic and pathologic mechanosensation from the distal colon. Cell, 3368-3385.e18.
37416774	not listed	Hu, M, et al. 2023. Defective Neurite Elongation and Branching in Nibp/Trappc9 Deficient Zebrafish and Mice. International Journal of Biological Sciences, 3226-3248.
37350129	1:1500	Glaser, EP, et al. 2023. Effects of Acute Ethanol Intoxication on Spinal Cord Injury Outcomes in Female Mice. Journal of Neurotrauma, 0.
37208933	1:500	Pukos, N, et al. 2023. Chronic Demyelination and Myelin Repair After Spinal Cord Injury in Mice: A Potential Link for Glutamatergic Axon Activity. Glia, 2093-2116.
36788334	1:1000	Lopez, J.A., et al. 2023. Caldendrin Represses Neurite Regeneration and Growth in Dorsal Root Ganglion Neurons. Scientific Reports, 2608.
36745682	1:5000	Tang, A.T., et al. 2023. Cell-Autonomous Requirement for ACE2 Across Organs in Lethal Mouse SARS-CoV-2 Infection. PLoS Biology, e3001989.
36370710	1:5000	Wang, X., et al. 2022. Driving Axon Regeneration By Orchestrating Neuronal And Non-Neuronal Innate Immune Responses Via The IFNγ-cGAS-STING Axis. Neuron, .
36284453	not listed	Cantu-Guerra, H.L., et al. 2022. Cochlear Hair Cell Innervation is Dependent on a Modulatory Function of Semaphorin-3A. Developmental Dynamics, 124-144.
36087584	1:300	Orr, B., et al. 2022. Activation and expansion of presynaptic signaling foci drives presynaptic homeostatic plasticity. Neuron, 3743-3759.
35568187	not listed	Madalena, K.M., et al. 2022. Genetic deletion of the glucocorticoid receptor in Cx3cr1+ myeloid cells is neuroprotective and improves motor recovery after spinal cord injury. Experimental neurology, 114114.
35552394	1:200	Wang, Y., et al. 2022. The WNT7A/WNT7B/GPR124/RECK signaling module plays an essential role in mammalian limb development. Development, .
35373589	1:1500	Stewart, A., et al. 2022. Advanced Age and Neurotrauma Diminish Glutathione and Impair Antioxidant Defense after Spinal Cord Injury. Journal of neurotrauma, 1075-1089.
34789880	1:500	Emanuel, A.J., et al. 2021. Cortical responses to touch reflect subcortical integration of LTMR signals. Nature, 680-701.
34731626	1:5000	Parpaite, T., et al. 2021. Patch-seq of mouse DRG neurons reveals candidate genes for specific mechanosensory functions. Cell reports, 109914.
34730998	1:1000	Xu, Q., et al. 2021. Astrocytes contribute to pain gating in the spinal cord. Science advances, 6287.
34637701	1:500	Lehnert, B.P., et al. 2021. Mechanoreceptor synapses in the brainstem shape the central representation of touch. Cell, 5608-5621.
33422552	1:2500	Stewartac, A.N., et al. 2021. Mitochondria exert age-divergent effects on recovery from spinal cord injury. Experimental Neurology, 113597.
33422552	1:2500	Stewart, A.N., et al. 2021. Mitochondria exert age-divergent effects on recovery from spinal cord injury. Experimental Neurology, 113597.
33333021	1:500	Huang, S., et al. 2020. Lymph nodes are innervated by a unique population of sensory neurons with immunomodulatory potential. Cell, 441-459.
32675174	1:1000	Wang, Z., et al. 2020. The Purinergic Receptor P2rx3 is Required for Spiral Ganglion Neuron Branch Refinement during Development. eNeuro, .
32554568	1:500	Neubarth, N.L., et al. 2020. Meissner Corpuscles and Their Spatially Intermingled Afferents Underlie Gentle Touch Perception. Science, eabb2751.
31862897	1:200	Morrey, J.D., et al. 2019. Zika virus infection causes temporary paralysis in adult mice with motor neuron synaptic retraction and evidence for proximal peripheral neuropathy. Scientific Reports, 19531.
31309927	1:400	Stavoe, A.K.H, et al. 2019. Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons.. Elife, e44219.
31248728	1:1000	Zheng, Y. , et al. 2019. Deep Sequencing of Somatosensory Neurons Reveals Molecular Determinants of Intrinsic Physiological Properties.. Neuron, 598-616.
31209173	1:2000	Jung, J.S., et al. 2019. Semaphorin-5B Controls Spiral Ganglion Neuron Branch Refinement during Development.. Journal of Neuroscience, 6425-6438.
30866042	1:400	Goel, M., et al. 2019. Differential expression and sub-cellular localization of Copines in mouse retina. Journal of Comparative Neurology, 2245-2262.
30726728	1:500-1:1000	Zhang, M., et al. 2019. Mechanically Activated Piezo Channels Mediate Touch and Suppress Acute Mechanical Pain Response in Mice. Cell Reports, 1419-1431.
30686732	1:500	Green, D.P., et al. 2019. A Mast-Cell-Specific Receptor Mediates Neurogenic Inflammation and Pain. Neuron, 412-420.
30478340	1:10000	Colina, C., et al. 2018. Selective tracking of FFAR3-expressing neurons supports receptor coupling to N-type calcium channels in mouse sympathetic neurons. Scientific reports, 17379.
30453022	1:500	Zhang, B., et al. 2018. Reducing age-dependent monocyte-derived macrophage activation contributes to the therapeutic efficacy of NADPH oxidase inhibition in spinal cord injury. Brain, Behavior, and Immunity, 139-150.
30406183	1:100	Micheva, K.D. , et al. 2018. Distinctive Structural and Molecular Features of Myelinated Inhibitory Axons in Human Neocortex.. eNeuro, .
29874580	1:1000	Kridsada, K., et al. 2018. Roof Plate-Derived Dorsal Midline Radial Glial-like Cells Promote Longitudinal Ascending Axon Growth of Rapidly Adapting Mechanoreceptors during Development. Cell Reports, 2928-2941.
29536590	1:5000	Harley, R.J., et al. 2018. Neuronal Cell Adhesion Molecule (NrCAM) is Expressed by Sensory Cells in the Cochlea and is Necessary for Proper Cochlear Innervation and Sensory Domain Patterning During Development. Developmental Dynamics, 934-950.
29476408	1:200	Zukor, K., et al. 2018. Zika virus-induced acute myelitis and motor deficits in adult interferon αβ/γ receptor knockout mice. Journal of NeuroVirology, 273-290.
29329211	1:200	Morrey, J.D., et al. 2018. Causation of Acute Flaccid Paralysis by Myelitis and Myositis in Enterovirus-D68 Infected Mice Deficient in Interferon αβ/γ Receptor Deficient Mice. Viruses, 33.
29279310	1:1000	Hesp, Z.C., et al. 2018. Proliferating NG2-Cell-Dependent Angiogenesis and Scar Formation Alter Axon Growth and Functional Recovery After Spinal Cord Injury in Mice. The Journal of Neuroscience, 1366-1382.
28817806	not listed	Ghitani, N., et al. 2017. Specialized Mechanosensory Nociceptors Mediating Rapid Responses to Hair Pull. Neuron, 944-954.
28647856	1:500	Shih, Y., et al. 2017. Glial and Neuronal Protein Tyrosine Phosphatase Alpha (PTPα) Regulate Oligodendrocyte Differentiation and Myelination. Journal Molecular Neuroscience, 329-343.
28381129	not listed	Orr, M.B., et al. 2017. Compression Decreases Anatomical and Functional Recovery and Alters Inflammation after Contusive Spinal Cord Injury. Journal of Neurotrauma, 2342-2352.
28264978	1:200	Freria, C.M., et al. 2017. Deletion of the fractalkine receptor, CX3CR1, improves endogenous repair, axon sprouting and synaptogenesis after spinal cord injury in mice. The Journal of Neuroscience, 3568-3587.
28162807	1:1000	François, A., et al. 2017. A Brainstem-Spinal Cord Inhibitory Circuit for Mechanical Pain Modulation by GABA and Enkephalins. Neuron, 822-839.
28041852	1:1000	Abraira, V.E., et al. 2017. The Cellular and Synaptic Architecture of the Mechanosensory Dorsal Horn. Cell, 295-310.
28168703	1:1000	Geary, M.B., et al. 2016. Erythropoietin accelerates functional recovery after moderate sciatic nerve crush injury. Muscle & Nerve, 143-151.
28002412	1:1000	Nonomura, K., et al. 2016. Piezo2 senses airway stretch and mediates lung inflation-induced apnoea. Nature, 176-181.
27707970	1:500	Fleming, M.S., et al. 2016. A RET-ER81-NRG1 Signaling Pathway Drives the Development of Pacinian Corpuscles. The Journal of Neuroscience, 10337-10355.
27596335	1:500	Zhang, B., et al. 2016. Age increases reactive oxygen species production in macrophages and potentiates oxidative damage after spinal cord injury. Neurobiology of Aging, 157-167.
26356988	1:400	Sajgo, S., et al. 2015. Dynamic expression of transcription factor Brn3b during mouse cranial nerve development. Journal of Comparative Neurology, 1033-1061.
25451251	1:5000	Yamada, H., et al. 2014. Transplantation of mature adipocyte-derived dedifferentiated fat cells promotes locomotor functional recovery by remyelination and glial scar reduction after spinal cord injury in mice. Biochemical and Biophysical Research Communications, 341-346.

Immunohistochemistry: Rat
PMID	Dilution	Publication
36759186	1:200	Arbat-Plana, A., et al. 2023. Massive Loss of Proprioceptive Ia Synapses in Rat Spinal Motoneurons after Nerve Crush Injuries in the Postnatal Period. eNeuro, .
33468672	1:200	Meregalli, C., et al. 2021. Pathogenic role of delta 2 tubulin in bortezomib-induced peripheral neuropathy. PNAS, .

Western Blot: Mouse
PMID	Dilution	Publication
37416774	not listed	Hu, M, et al. 2023. Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and mice. International journal of biological sciences, 3226-3248.

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Anti-NFH (Neurofilament Heavy Chain) Antibody