Name: Anti-NFH (Neurofilament Heavy Chain) Antibody
Brand: Aves Labs
SKU: NFH
Price: 265.0 USD
Availability: InStock

NFH protein was purified from bovine spinal cords following the method of Delacourte et al. ["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion” (1980). Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFH protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. These antibodies have been found to cross-react with NFH protein from a variety of species, including human, mouse and rat.

Clonality

Polyclonal

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

NEFH KIAA0845 NFH

Molecular Weight

200-220 kDa

Go to Full Product Details

Cortical neuron from a neo-natal mouse brain culture immunocytochemically stained for NF-H immunoreactivity (Aves Labs Cat.# NFH; 1:5000 dilution). Secondary antibody (fluorescein-labeled goat anti-chicken IgY) from Jackson Immunoresearch (1:1000 dilution)

Product Details

Target

Neurofilament Heavy Chain (NFH)

Target Description

Human Neurofilament Heavy Chain (NFH) is a 115,378 dalton protein (1072 amino acids) that forms part of the neuronal cytoskeleton. NFH is physically associated with 10 nm intermediate filaments and is said to be one of the "neurofilament triplet" proteins, although it probably serves more to stabilize the cytoskeleton rather than as an integral structural component. NFH immunoreactivity is found in neuronal somata, dendrites and axons.

Concentration

1 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

NEFH KIAA0845 NFH

Molecular Weight

200-220 kDa

Antigen

Native NF-H purified from bovine spinal cord

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313552

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Physical State

Liquid

Production Notes

Chickens were immunized with NF-H protein purified from adult bovine brains. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in the buffer described above, and then filter-sterilized.

Dilution Ranges

WB: 1:2000-1:5000
IHC: 1:2000-1:5000
ICC: 1:2000-1:5000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:50000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Shipping

Ice packs

Synonyms

Neurofilament heavy polypeptide (NF-H) (200 kDa neurofilament protein) (Neurofilament triplet H protein)

UniProt Details

UniProt (Immunogen Species): P12036

Citations (22)

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Anti-NFH (Neurofilament Heavy Chain) Antibody