Our Anti-NF-kB p65 (Ser316) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse NF-kB p65 (Ser316) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of 20’ UV treated HeLa lysate showing specific immunolabeling of the ~65 kDa NF-κB p65 phosphorylated at Ser316 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is eliminated by blot treatment with lambda phosphatase (lambda-Ptase, 1200 units for 30 min).
p65, also known as RelA, is one of the five members of the NF-kB family. The p65 and p50 heterodimer is the predominant form of NF-kB, where p65 is the major subunit (Gilmore et al, 2006). There have been 13 identified phosphorylation sites on p65, including Ser-316 which is induced by IL-1β (Viatour et al, 2005 and Wang et al, 2015). Phosphorylation of Ser-316 is essential for NF-kB nuclear transportation, NF-kB-dependent gene regulation, cytokine/chemokine secretion, and other biological functions (Wang et al, 2015). Ser-316 has also been demonstrated to co-phosphorylate with either Ser-529 or Ser-536 to regulate the majority of NF-kB targeted genes while having its own unique regulation function (Wang et al, 2015). CKI kinase specifically targets phosphorylation at Ser-316 on p65 (Wang et al, 2015). CKI is involved in regulation of membrane transport, cell division, DNA repair, and nuclear localization by phosphorylating its target proteins (Price et al, 2006).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
RELA
62 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser316 of mouse NF-kB p65, conjugated to keyhole limpet hemocyanin (KLH).
Human, Mouse
Bovine, Canine, Goat, Guinea Pig, Rat
AB_2756814
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for endogenous levels of the ~65 kDa NF-kB p65 protein phosphorylated at Ser316. Immunolabeling is eliminated with λ-phosphatase treatment.
Phosphorylated
Ser316
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody, MGC131774 antibody, NF kappa B p65delta3 antibody, NFKB3 antibody, Nuclear Factor NF Kappa B p65 Subunit antibody, Nuclear factor NF-kappa-B p65 subunit antibody, Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody, OTTHUMP00000233473 antibody, OTTHUMP00000233474 antibody, OTTHUMP00000233475 antibody, OTTHUMP00000233476 antibody, OTTHUMP00000233900 antibody, p65 antibody, p65 NF kappaB antibody, p65 NFkB antibody, relA antibody, TF65_HUMAN antibody, Transcription factor p65 antibody, v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) antibody, V rel avian reticuloendotheliosis viral oncogene homolog A antibody, v rel reticuloendotheliosis viral oncogene homolog A (avian) antibody, V rel reticuloendotheliosis viral oncogene homolog A nuclear factor of kappa light polypeptide gene enhancer in B cells 3 p65 antibody
Viatour, P., Merville, M.P., Bours, V. and Chariot, A., 2005. Phosphorylation of NF-κB and IκB proteins: implications in cancer and inflammation. Trends in Biochemical Sciences, 30(1), pp.43-52. PMID: 15653325
Wang, B., Wei, H., Prabhu, L., Zhao, W., Martin, M., Hartley, A.V. and Lu, T., 2015. Role of novel serine 316 phosphorylation of the p65 subunit of NF-κB in differential gene regulation. Journal of Biological Chemistry, 290(33), pp.20336-20347. PMID: 26082493
Price, M.A., 2006. CKI, there's more than one: casein kinase I family members in Wnt and Hedgehog signaling. Genes & Development, 20(4), pp.399-410. PMID: 16481469
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