Our Anti-Neurofilament NF-M primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, chicken, human, mouse, and rat Neurofilament NF-M and is Protein G purified. It is great for use in WB, IHC, ICC.
Western blot of rat cortical lysate showing specific immunolabeling of the ~145 kDa NF-M protein.
Anti-Neurofilament NF-M Antibody
Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (2).
Protein G Purified
ICC, IHC, WB
Preparation containing the extreme C-terminus of rat NF-M, expressed in and purified from E. Coli.
Bovine, Chicken, Human, Mouse, Rat
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Specific for endogenous levels of the ~145 kDa neurofilament M protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
150 kDa Neurofilament Medium antibody, 160 kDa neurofilament protein antibody, Medium polypeptide 150kDa antibody, NEF3 antibody, Neurofilament 3 antibody, Neurofilament 3 medium antibody, Neurofilament medium polypeptide antibody, Neurofilament protein M antibody, Neurofilament triplet M protein antibody, Neurofilament-3 (150 kD medium) antibody, NF-M antibody, NFM antibody
Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. Journal of Neuroscience Research 30:47-62. PMID: 1724473
Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neuroscience Letters 320(3):156-60. PMID: 11852185
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