Name: Anti-Neurofilament NF-M Antibody
Brand: PhosphoSolutions
SKU: 1455-NFM
Price: 360.0 USD
Availability: InStock

Our Anti-Neurofilament NF-M primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, chicken, human, mouse, and rat Neurofilament NF-M and is total IgY fraction. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Format

Total IgY fraction

Gene Name

NEFM

Molecular Weight

145 kDa

Go to Full Product Details

Immunofluorescence of a section of rat cerebellum labeled with anti-Neurofilament-M (cat. 1455-NFM, 1:1000, red) and anti-CNP(green). The anti-NFM labels the network of axons of basket neurons and other neurons. The blue is DAPI staining of nuclear DNA.

Immunostaining of cultured rat neurons and glia stained with anti-NFM antibody (cat. 1455-NFM, red, 1:1000) and DNA is stained blue.

Western blot of rat cortical lysate showing specific immunolabeling of the ~145 kDa NF-M protein.

Volume: 100 µL

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Price:

$360

Quantity:

Bulk Order Anti-Neurofilament NF-M Antibody

Product Details

Target

Neurofilament NF-M

Target Description

Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (Harris et al., 1991). NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (Hu et al., 2002).

Format

Total IgY fraction

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

NEFM

Molecular Weight

145 kDa

Antigen

Preparation containing the extreme C-terminus of rat NF-M, expressed in and purified from E. Coli.

Species Reactivity

Bovine, Chicken, Human, Mouse, Rat

Antibody Registry ID

AB_2492164

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Total IgY fraction

Buffer

PBS + 10 mM NaN₃

Dilution Ranges

WB: 1:5000
WB Brain: 1:5000
IHC: 1:1000
ICC: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~145 kDa Neurofilament M protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

150 kDa Neurofilament Medium antibody, 160 kDa neurofilament protein antibody, Medium polypeptide 150kDa antibody, NEF3 antibody, Neurofilament 3 antibody, Neurofilament 3 medium antibody, Neurofilament medium polypeptide antibody, Neurofilament protein M antibody, Neurofilament triplet M protein antibody, Neurofilament-3 (150 kD medium) antibody, NF-M antibody, NFM antibody

UniProt Details

UniProt (Human): P07197
UniProt (Immunogen Species): P12839

NCBI Gene ID

24588

Product Specific Protocols

Western Blot Protocol: Download

General References

Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. Journal of Neuroscience Research 30:47-62. PMID: 1724473
Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neuroscience Letters 320(3):156-60. PMID: 11852185

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Anti-Neurofilament NF-M Antibody

Our Anti-Neurofilament NF-M primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, chicken, human, mouse, and rat Neurofilament NF-M and is total IgY fraction. It is great for use in WB, IHC, ICC.