Anti-Neurofilament NF-H Antibody

Name: Anti-Neurofilament NF-H Antibody
Brand: PhosphoSolutions
SKU: 1451-NFH
Price: 360.0 USD
Availability: InStock

from PhosphoSolutions Datasheet Trial Size

Our Anti-Neurofilament NF-H primary antibody from PhosphoSolutions is chicken polyclonal. It detects all known mammal and chicken Neurofilament NF-H and is total IgY fraction. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Chicken

Applications

ICC, IHC, WB

Host Species

Chicken

Format

Total IgY fraction

Gene Name

NEFH

Molecular Weight

200 kDa

Go to Full Product Details

Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament H (cat. 1451-NFH, 1:25,000, red) in nuclei of neurons and specific labeling of GFAP( cat. 620-GFAP , 1:5000, green) in astrocytes and other glial cells, and DAPI staining of nuclear DNA.

Immunofluorescence of a section of rat cerebellum showing specific labeling of the axons with anti-neurofilament H antibody (cat. 1451-NFH, green, 1:25,000). The section is colabeled with anti-calbindin ( cat. 302-CALB , red, 1:1000) labeling the dendrites of Purkinje cells. The blue is DAPI staining nuclear DNA.

Immunostaining of mixed cultured rat neurons and glia stained with anti-NFH antibody (cat. 1451-NFH, red, 1:25,000) and anti-NFL antibody (cat. 1452-NFL, green, 1:100).

Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.

Volume: 100 µL

Variant

Price:

$360

Quantity:

Bulk Order Anti-Neurofilament NF-H Antibody

Product Details

Target

Neurofilament NF-H

Target Description

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (Harris et al., 1991). NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) (Mendonca et al., 2005) and Alzheimer's disease (Hu et al., 2002).

Format

Total IgY fraction

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

NEFH

Molecular Weight

200 kDa

Antigen

Purified bovine NF-H

Species Reactivity

Chicken

Antibody Registry ID

AB_2492160

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Total IgY fraction

Buffer

PBS + 10 mM NaN3.

Dilution Ranges

WB: 1:50000
WB Brain: 1:50000
IHC: 1:5000-1:25,000
ICC: 1:25,000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~200 kDa Neurofilament H protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

200 kDa neurofilament protein antibody, CMT2CC antibody, Nefh antibody, Neurofilament heavy polypeptide 200kDa antibody, Neurofilament heavy polypeptide antibody, Neurofilament triplet H protein antibody, NF H antibody, NF-H antibody, NFH antibody, NFH_HUMAN antibody

UniProt Details

UniProt (Human): P12036
UniProt (Immunogen Species): P12036

NCBI Gene ID

4744

General References

Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.PMID: 1724473
Mendonca DM, Chimelli L, Martinez AM. (2005) Quantitative evidence for neurofilament heavy subunit aggregation in motor neurons of spinal cords of patients with amyotrophic lateral sclerosis. Braz J Med Biol Res. 38(6):925-933. PMID: 15933787
Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320(3):156-60. PMID: 11852185

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Anti-Neurofilament NF-H Antibody

Our Anti-Neurofilament NF-H primary antibody from PhosphoSolutions is chicken polyclonal. It detects all known mammal and chicken Neurofilament NF-H and is total IgY fraction. It is great for use in WB, IHC, ICC.