Anti-Nestin Antibody (4D11)

3 Citations

Our Anti-Nestin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Nestin and is Protein G purified. It is great for use in WB, IHC, ICC.

Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~220-240 kDa nestin doublet.
Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (cat. 1435-NES, red, 1:500) and anti-MAP2 antibody (cat. 1100-MAP2, green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
Immunostaining of cultured neonatal rat neurons and glia stained with anti-nestin antibody (cat. 1435-NES, red, 1:500) and anti-vimentin antibody (cat. 2105-VIM, green, 1:500). The blue is Hoechst staining for nuclear DNA. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
Immunolabeling of the coronal section of an embryonic day 15.5 mouse cortex showing specific labeling of Nestin (cat. 1435-NES, 1:500; green). Nuclei stained with DAPI (blue). Image kindly provided by Alyssa Treptow, University of California, Riverside.
Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~220-240 kDa nestin doublet.
Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~220-240 kDa nestin doublet.
Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (cat. 1435-NES, red, 1:500) and anti-MAP2 antibody (cat. 1100-MAP2, green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
Immunostaining of cultured neonatal rat neurons and glia stained with anti-nestin antibody (cat. 1435-NES, red, 1:500) and anti-vimentin antibody (cat. 2105-VIM, green, 1:500). The blue is Hoechst staining for nuclear DNA. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
Immunolabeling of the coronal section of an embryonic day 15.5 mouse cortex showing specific labeling of Nestin (cat. 1435-NES, 1:500; green). Nuclei stained with DAPI (blue). Image kindly provided by Alyssa Treptow, University of California, Riverside.

Human, Mouse, Rat
ICC, IHC, WB
Mouse

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Data Sheet
Trial Size Trial Size

SKU: 1435-NES

Volume: 100 µL
Price:
$430

Product Specific References for Applications and Species

  • Immunocytochemistry: Mouse
  • Immunohistochemistry: Mouse
Immunocytochemistry: Mouse
PMID Dilution Publication
33326788 Not listed Wang, J., et al. 2020. Talpid3-Mediated Centrosome Integrity Restrains Neural Progenitor Delamination to Sustain Neurogenesis by Stabilizing Adherens Junctions. Cell Reports, 33(11), p.108495.
Immunohistochemistry: Mouse
PMID Dilution Publication
33871731 Not listed Ka, M., et al. 2021. MACF1, Involved in the 1p34. 2p34. 3 Microdeletion Syndrome, is Essential in Cortical Progenitor Polarity and Brain Integrity. Cellular and Molecular Neurobiology. p.1-18.
28641285 Not listed Thakur, V., et al. 2017. Role of Glycyrrhizin in the Reduction of Inflammation in Diabetic Kidney Disease. Nephron. 137(2):137-147.

Product Specific Protocols

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