Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~220-240 kDa nestin doublet.
Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells (Lendahl et al., 1990). Nestin was originally identified as a result of the production of a series of monoclonal antibodies directed against epitopes expressed on formalin fixed embryo day 15 rat spinal cord (Hockfield et al., 1985)). One of these antibodies, called Rat 401, stained fibrous profiles in the developing nervous system, but not in the mature nervous system. By screening bacteriophage expression libraries with Rat 401, Lendahl et al. were able to isolate a cDNA encoding the protein to which Rat 401 antibody bound. The protein proved to be an unusual member of the intermediate filament family, containing an alpha-helical region homologous to that found in keratin and neurofilament subunits.
Protein G Purified
ICC, IHC, WB
Recombinant human Nestin expressed in and purifeid from E. coli.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Protein G purified culture supernatant
PBS + 50% glycerol and 5 mm NaN3
WB Brain: 1:2000
Specific for endogenous levels of the ~220-240 kDa nestin doublet.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
ESTM 46 antibody, FLJ 21841 antibody, FLJ21841 antibody, Intermediate filament protein antibody, Nbla00170 antibody, nes antibody, NEST_HUMAN antibody, Nestin antibody