Anti-Nav1.8 Na+ Channel Antibody (N134/12)
Our Anti-Nav1.8 Na+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N134/12. It is KO validated, detects human, mouse, and rat Nav1.8 Na+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Immunostaining of a 7 µm thick DRG neuron section from a 3 month old rat labeling NaV1.8 (green, 1:2000, Cat. 75-166), IB4 (red), and nuclei is stained with DAPI (blue). The image is kindly provided by Cristian Acosta, Universidad Nacional de Cuyo, Mendoza, Argentina.
Product Specific References for Applications and Species
- Immunocytochemistry: Rat
- Immunohistochemistry: Human | Mouse | Rat
- MicroArray: Mouse
- Western Blot: Mouse | Rat
- Additional Publications: Unspecified
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 34431981 | 1:500 | Isensee, J., et al. . Depolarization induces nociceptor sensitization by CaV1.2-mediated PKA-II activation. Journal of Cell Biology, e202002083. |
| 31501450 | 1:200 | Vysokov, N., et al. . The role of Na V channels in synaptic transmission after axotomy in a microfluidic culture platform. Scientific Reports, 12915. |
| 24453337 | 1:2000 | Acosta, C., et al. . TREK2 expressed selectively in IB4-binding C-fiber nociceptors hyperpolarizes their membrane potentials and limits spontaneous pain.. Journal of Neuroscience, 1494-509. |
| 24278311 | 1:500 | Tsantoulas, C., et al. . Probing functional properties of nociceptive axons using a microfluidic culture system.. PLoS One, e80722. |
| 21118538 | 1:100 | Persson, A.K., et al. . Sodium-calcium exchanger and multiple sodium channel isoforms in intra-epidermal nerve terminals. . Molecular Pain, 1744-8069. |
| Immunohistochemistry: Human | ||
| PMID | Dilution | Publication |
| 22794211 | Kim, Y.S., et al. . Expression of transient receptor potential ankyrin 1 in human dental pulp. Journal of Endodontics, 1087-92. | |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 38124021 | 1:1000 | Han, X, et al. 2023. Opioid-induced hyperalgesia and tolerance are driven by HCN ion channels. The Journal of Neuroscience, . |
| 37940033 | 1:200 | Gupta, DP, et al. 2023. EBP50 is a key molecule for the Schwann cell-axon interaction in peripheral nerves. Progress in Neurobiology, 102544. |
| 34967003 | 1:500 | Mason, B.N., et al. . Prolactin signaling modulates stress-induced behavioral responses in a preclinical mouse model of migraine. Headache, 11-25. |
| 32077526 | Song, G.J., et al. . Loss-of-function of EBP50 is a new cause of hereditary peripheral neuropathy: EBP50 functions in peripheral nerve system. Glia, 1794-809. | |
| 31290157 | 1:500 | Lainez, S., et al. . HCN3 ion channels: roles in sensory neuronal excitability and pain. The Journal of Physiology, 4661-75. |
| 29697531 | 1:1000 | Tsantoulas, C., et al. . Mice lacking Kcns1 in peripheral neurons show increased basal and neuropathic pain sensitivity. Pain, 1641-51. |
| 25995484 | 1:10 | Lu, V.B., et al. . A 3.7 kb fragment of the mouse Scn10a gene promoter directs neural crest but not placodal lineage EGFP expression in a transgenic animal.. Journal of Neuroscience, 8021-34. |
| 25600872 | 1:250 | François, A., et al. . The Low-Threshold Calcium Channel Cav3.2 Determines Low-Threshold Mechanoreceptor Function.. Cell Report, 370-82. |
| 24903831 | 1:200 | Montersino, A., et al. . Tetrodotoxin-resistant voltage-gated sodium channel Nav 1.8 constitutively interacts with ankyrin G.. Journal of Neurochemistry, 33-41. |
| 23884957 | 1:500 | Lippoldt, E.K., et al. . Artemin, a glial cell line-derived neurotrophic factor family member, induces TRPM8-dependent cold pain.. Journal of Neuroscience, 12543-52. |
| 23407943 | 1:500 | Knowlton, W.M., et al. . A sensory-labeled line for cold: TRPM8-expressing sensory neurons define the cellular basis for cold, cold pain, and cooling-mediated analgesia. Journal of Neuroscience, 2837-48. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 36478541 | 1:2000 | Messina, D.N., et al. 2022. Age-dependent and Modality-specific Changes in the Phenotypic Markers Nav1.8, ASIC3, P2X3 and TRPM8 in Male Rat Primary Sensory Neurons During Healthy Aging. Biogerontology, 111-136. |
| 1:1000 | Tsantoulas, C., et al. 2022. HCN2 Ion Channels Drive Pain in Rodent Models of Migraine. The Journal of Neuroscience, 7513-7529. | |
| 25609640 | not listed | Ritter, D.M., et al. . Dysregulation of Kv3.4 channels in dorsal root ganglia following spinal cord injury.. Journal of Neuroscience, 1260-73. |
| 23642408 | 0.25 µg/mL | Liang, L., et al. . Protein kinase B/Akt is required for complete Freund''s adjuvant-induced upregulation of Nav1.7 and Nav1.8 in primary sensory neurons.. The Journal of Pain, 638-47. |
| 21965668 | 1:500 | Ruangsri, S., et al. . Relationship of axonal voltage-gated sodium channel 1.8 (NaV1.8) mRNA accumulation to sciatic nerve injury-induced painful neuropathy in rats. Journal of Biological Chemistry, 39836-47. |
| 21118538 | 1:100 | Persson, A.K., et al. . Sodium-calcium exchanger and multiple sodium channel isoforms in intra-epidermal nerve terminals. . Molecular Pain, 1744-8069. |
| MicroArray: Mouse | ||
| PMID | Dilution | Publication |
| 34931070 | 1:500 | Yang, N.J., et al. . Anthrax toxins regulate pain signaling and can deliver molecular cargoes into ANTXR2+ DRG sensory neurons. Nature Neuroscience, 168-79. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 33636225 | 1:1000 | Sun, L., et al. . MicroRNA-96 is required to prevent allodynia by repressing voltage-gated sodium channels in spinal cord. Progress in Neurobiology, 102024. |
| 28515230 | 1:1000 | Isensee, J., et al. . Crosstalk from cAMP to ERK1/2 emerges during postnatal maturation of nociceptive neurons and is maintained during aging. Journal of Cell Science, 2134-46. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 23792947 | 1:1000 | Zhao, X., et al. 2013. A long noncoding RNA contributes to neuropathic pain by silencing Kcna2 in primary afferent neurons.. Nature Neuroscience, 1024-1031. |
| 23642408 | 1:1000 | Liang, L., et al. . Protein kinase B/Akt is required for complete Freund''s adjuvant-induced upregulation of Nav1.7 and Nav1.8 in primary sensory neurons.. The Journal of Pain, 638-47. |
| 20026672 | not listed | Hoffman, E.M., et al. . Fixative Composition Alters Distributions of Immunoreactivity for Glutaminase and Two Markers of Nociceptive Neurons, Nav1.8 and TRPV1, in the Rat Dorsal Root Ganglion. Journal of Histochemistry and Cytochemistry, 329-44. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 21729793 | Spofford, C.M., et al. . Evaluation of leukemia inhibitory factor (LIF) in a rat model of postoperative pain. The Journal of Pain, 819-32. | |