Western blot image of human endothelial cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blots were probed with anti-N-cadherin (a.a. 811-824) (lanes 1 & 2) and anti-unphosphorylated N-cadherin (Tyr-820) (lanes 3 & 4).
Anti-N-Cadherin (C-terminal region) Antibody
Bulk Order Anti-N-Cadherin (C-terminal region) Antibody
N-Cadherin (C-terminal region)
Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.
Antigen Affinity Purified
ICC, IP, WB
unphosphorylated N-Cadherin synthetic peptide (coupled to carrier protein) corresponding to amino acids from the C-terminal region of human N-cadherin. This sequence is conserved in rat and mouse N-cadherin, and has three amino acid differences from the conserved region in R-cadherin.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
The purified antibody detects a band at 130 kDa* in western blots of human endothelial cells and mouse brain tissue, and does not cross-react with E-cadherin. The antibody also works for ELISA, immunoprecipitation, and immunocytochemistry.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.