Anti-Myosin Light Chain (Ser-1), Phosphospecific Antibody

Name: Anti-Myosin Light Chain (Ser-1), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: MP3461
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

4 Citations

Product Type

Primary Antibody

Species Reactivity

Bovine, Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

MYL12A

Molecular Weight

Go to Full Product Details

Immunocytochemical labeling of phosphorylated MLC in paraformaldehyde fixed A7r5 cells. The cells were dual-labeled with anti-MLC (MM3441; middle) and anti-MLC (MP4201; top left), anti-MLC (Ser-19) (MP4221; middle left) and anti-MLC (Ser-1) (MP3461; bottom left). Goat anti-Mouse DyLight® 488 and Goat anti-Rabbit DyLight® 594 were used for detection of primary antibodies. The overlay of staining patterns are shown to the right.

Western blot analysis of C2C12 cells untreated (lanes 1, 3, 5, & 7) or treated with Lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with monoclonal anti-MLC20 (clone MY-21) (lanes 1 & 2), polyclonal anti-MLC (Ser-19) phosho-specific (lanes 3 & 4), anti-MLC (Ser-1) phosho-specific (lanes 5 & 6), or anti-MLC (a.a. 11-22) (lanes 7 & 8) .

Volume: 100 µL

Variant

Price:

$309

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Bulk Order Anti-Myosin Light Chain (Ser-1), Phosphospecific Antibody

Product Details

Target

Myosin Light Chain (Ser-1)

Target Description

Both smooth muscle and nonmuscle myosin II activity is regulated by the phosphorylation state of the myosin regulatory light chain (MLC, MRLC, MLC20, Myl9). Phosphorylation of MLC at Thr-18 and Ser-19 activates myosin II motor activity and increases myosin filament stability. This activation has important roles in various cell motile processes. By contrast, other phosphorylation sites on MLC may inhibit myosin II activity. PKC phosphorylates Ser-1/Ser-2 and Thr-9 in MLC, and this phosphorylation decreases activated myosin II interaction with actin, as well as inhibits MLC interaction with the activation site kinase, myosin light-chain kinase. The Ser-1/Ser-2 region may be the major inhibitory site since Ser-1 is phosphorylated during PDGF-induced stress fiber disassembly and expression of unphosphorylatable MLC20 at the Ser-1/Ser-2 site suppresses this disassembly. Thus, inhibition of myosin II activity through phosphorylation of Ser-1/Ser-2 may have important roles in growth factor-induced reorganization of actomyosin filaments.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

MYL12A

Molecular Weight

Antigen

Phospho-MLC (Ser-1) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-1 in bovine myosin regulatory light chain 9 (Myl9). This peptide sequence is highly conserved in human, rat, and mouse Myl9, and in other smooth muscle and non-muscle MLCs.

Species Reactivity

Bovine, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 20 kDa* band corresponding to the molecular weight of myosin light chain in human A431 treated with EGF or calyculin A. In addition, the antibody detects the 20 kDa myosin light chain in mouse C2C12, rat PC12, and rabbit fibroblasts treated with calyculin A.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-1

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

MLC20, RLC-C; Mylc2c; Myl9, MLC2, MRLC1, MYRL2; MLCB; MRCL3; MRLC3; MYL2B; MYL12A, myosin

UniProt Details

UniProt (Human): P19105
UniProt (Immunogen Species): P19105

Product Specific References for Applications and Species

Immunocytochemistry: Human
Immunohistochemistry: Rat
Western Blot: Human

Immunocytochemistry: Human
PMID	Dilution	Publication
32502416	not listed	Aguilar-Cuenca, R, et al. 2020. Tyrosine Phosphorylation of the Myosin Regulatory Light Chain Controls Non-muscle Myosin II Assembly and Function in Migrating Cells. Current biology : CB, 2446-2458.
22136066	not listed	Beach, JR, et al. 2011. Analysis of the role of Ser1/Ser2/Thr9 phosphorylation on myosin II assembly and function in live cells. BMC cell biology, 52.

Immunohistochemistry: Rat
PMID	Dilution	Publication
37645613	not listed	Yadav, P, et al. 2023. EZH2 and matrix co-regulate phenotype and KCNB2 expression in bladder smooth muscle cells. American Journal of Clinical and Experimental Urology, 293-303.

Western Blot: Human
PMID	Dilution	Publication
21695279	1:2000	Aguilar, HN, et al. 2011. Phos-tag-based analysis of myosin regulatory light chain phosphorylation in human uterine myocytes. PloS one, e20903.

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Anti-Myosin Light Chain (Ser-1), Phosphospecific Antibody