Primary Antibody
Bovine, Human, Mouse, Rat
ICC, WB
Rabbit
Antigen Affinity Purified
MYL12A
20


Western blot analysis of C2C12 cells untreated (lanes 1, 3, 5, & 7) or treated with Lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with monoclonal anti-MLC20 (clone MY-21) (lanes 1 & 2), polyclonal anti-MLC (Ser-19) phosho-specific (lanes 3 & 4), anti-MLC (Ser-1) phosho-specific (lanes 5 & 6), or anti-MLC (a.a. 11-22) (lanes 7 & 8) .
Product Specific References for Applications and Species
Immunocytochemistry: Human | ||
PMID | Dilution | Publication |
32502416 | not listed | Aguilar-Cuenca, R, et al. 2020. Tyrosine Phosphorylation of the Myosin Regulatory Light Chain Controls Non-muscle Myosin II Assembly and Function in Migrating Cells. Current biology : CB, 2446-2458. |
22136066 | not listed | Beach, JR, et al. 2011. Analysis of the role of Ser1/Ser2/Thr9 phosphorylation on myosin II assembly and function in live cells. BMC cell biology, 52. |
Immunohistochemistry: Rat | ||
PMID | Dilution | Publication |
37645613 | not listed | Yadav, P, et al. 2023. EZH2 and matrix co-regulate phenotype and KCNB2 expression in bladder smooth muscle cells. American Journal of Clinical and Experimental Urology, 293-303. |
Western Blot: Human | ||
PMID | Dilution | Publication |
21695279 | 1:2000 | Aguilar, HN, et al. 2011. Phos-tag-based analysis of myosin regulatory light chain phosphorylation in human uterine myocytes. PloS one, e20903. |