Anti-Myosin 7B/MHC14 (Hinge region) Antibody

Name: Anti-Myosin 7B/MHC14 (Hinge region) Antibody
Brand: PhosphoSolutions
SKU: MP4551
Price: 255.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

MYH7B

Molecular Weight

215

Go to Full Product Details

Immunocytochemical labeling of MYH7B in rat PC12 cells differentiated with NGF. The cells were probed with MYH7B (Hinge region) rabbit polyclonal antibody (MP4551) in the absence (left) or presence (right) of blocking peptide (MX4555). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

Western blot analysis MYH7B in mouse brain (lane 1 & 2) and mouse extraocular muscle (lane 3 & 4). The blot was probed with rabbit polyclonal anti-MYH7B/MHC14 (Hinge region) at 1:500 in absence (lanes 1 & 3) and presence of MYH7B blocking peptide (MX4555; lanes 2 & 4).

Volume: 100 µL

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$255

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Product Details

Target

Myosin 7B/MHC14 (Hinge region)

Target Description

Myosin is a highly conserved, ubiquitous protein found in all eukaryotic cells, where it provides the motor function for diverse movements such as cytokinesis, phagocytosis, and muscle contraction. All myosins contain an amino-terminal motor/head domain and a carboxy-terminal tail domain. The class II myosins, consist of the conventional two-headed myosins that form filaments and are composed of two myosin heavy chain (MYH) subunits and four myosin light chain subunits. There are 15 MYH genes identified as Class II myosins and these include six skeletal muscle MYHs (MYH1, MYH2, MYH3, MYH4, MYH8, MYH13), three cardiac MYHs (MYH6, MYH7, MYH7B) and two non-muscle MYHs (MYH9, MYH10), as well as a smooth muscle MYH (MYH11).

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

MYH7B

Molecular Weight

215

Antigen

A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the hinge region from mouse MYH7B/MHC14. This sequence is highly conserved in rat and human MYH7B, and has less than 50% identity with other MYH family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:300

Conjugate

Unconjugated

Specificity

The antibody detects a 215 kDa* protein corresponding to the apparent molecular mass of MYH7B on SDS-PAGE immunoblots of mouse extraocular muscle and brain.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Myh7b, MYH14, Myosin cardiac muscle

UniProt Details

UniProt (Human): A7E2Y1
UniProt (Immunogen Species): A2AQP0

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Myosin 7B/MHC14 (Hinge region) Antibody