Name: Anti-Myelin Basic Protein Antibody
Brand: Aves Labs
SKU: MBP
Price: 425.0 USD
Availability: InStock

An antipeptide antibody was generated in chickens against a sequence shared between the mouse (NP_034907) and human (NP_002376) gene products. Antibodies were affinity-purified and the concentration adjusted to 100 µg/mL.

Clonality

Polyclonal

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

MBP

Molecular Weight

Can recognize multiple bands between 14-22 kDa, corresponding to different MBP isoforms

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Immunohistochemical staining of MBP (RED) in the hippocampal region of a transgenic adult mouse brain. This particular transgenic mouse expresses low levels of GFP autofluorescence under control of an actin promoter region. The anti-MBP antibody (Aves Labs) was used at a 1:1000 dilution. Dr. Felix Eckenstein, University of Vermont.

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product. BLUE shows DAPI nuclear staining. Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.

Adult mouse cortical cultures were stained with chicken anti-MBP antibody (Aves Labs, GREEN, 1:1000 dilution) and rabbit anti-GFAP antibody (EnCorBio, RED; 1:1000 dilution). Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.

MBP: frozen sections; all washes with Triton 0.5%; Region: Striatum; Blocking: BlockHen 1%; Secondary antibody: anti-chicken FITC (Aves); Epifluorescence microscope; 40x magnification.

Chicken anti-Myelin Basic Protein (MBP) (Green) and Mouse anti-GFAP (Red) with nuclei stained with Hoechst dye (Blue).

Immunohistochemical staining of MBP (RED) in the hippocampal region of a transgenic adult mouse brain. This particular transgenic mouse expresses low levels of GFP autofluorescence under control of an actin promoter region. The anti-MBP antibody (Aves Labs) was used at a 1:1000 dilution. Dr. Felix Eckenstein, University of Vermont.

Volume: 1000 µL

Variant

Price:

$425

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Product Details

Target

Myelin Basic Protein (MBP)

Target Description

Human Myelin Basic Protein (MBP) is a 20,115 dalton protein (186 amino acids) found in myelin of the CNS and PNS. Expressed as an intracellular protein by oligodendrocytes and Schwann cells, MBP aids in the compaction and stability of myelin.

Concentration

100 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

MBP

Molecular Weight

Can recognize multiple bands between 14-22 kDa, corresponding to different MBP isoforms

Antigen

Synthetic peptide

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313550

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Physical State

Liquid

Production Notes

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the MBP gene product shared between the human (NP_002376, NCBI) and mouse (NP_034907, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. The concentrations of the eluates were then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Dilution Ranges

WB: 1:1000-1:2000
IHC: 1:1000-1:2000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Shipping

Ice packs

Synonyms

Myelin basic protein (MBP) (Myelin A1 protein) (Myelin membrane encephalitogenic protein)

UniProt Details

UniProt (Immunogen Species): P02686

Citations (12)

Yamada H, Ito D, Oki Y, Kitagawa M, Matsumoto T, Watari T, Kano K (2014), 'Transplantation of mature adipocyte-derived dedifferentiated fat cells promotes locomotor functional recovery by remyelination and glial scar reduction after spinal cord injury in mice.' Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2014.10.082.
Liu J, Song N, Guardiola J, Roman J, Yu J (2016), 'Slowly Adapting Sensory Units Have More Receptors in Large Airways than in Small Airways in Rabbits.' Frontiers in Pysciology. 10.3389/fphys.2016.00588.
Hegde S, Capell WR, Ibrahim BA, Klett J, Patel NS, Sougiannis AT, Kelly MP (2016), 'Phosphodiesterase 11A (PDE11A), Enriched in Ventral Hippocampus Neurons, is Required for Consolidation of Social but not Nonsocial Memories in Mice.' Neuropsychopharmacology. 10.1038/npp.2016.106.
Araújo SES, Mendonça HR, Wheeler NA, Campello-Costa P, Jacobs KM, Gomes FCA, Fox MA, Fuss B (2017), 'Inflammatory demyelination alters subcortical visual circuits.' Jounral of Neuroinflammation. 10.1186/s12974-017-0936-0.
Choquet K, Yang S, Moir RD, Forget D, Larivière R, Bouchard A, Poitras C, Sgarioto N, Dicaire MJ, Noohi F, Kennedy TE, Rochford J, Bernard G, Teichmann M, Coulombe B, Willis IM, Kleinman CL, Brais B (2017), 'Absence of neurological abnormalities in mice homozygous for the Polr3a G672E hypomyelinating leukodystrophy mutation.' Molecular Brain. 10.1186/s13041-017-0294-y.
Beirowski B, Wong KM, Babetto E, Milbrandt J (2017), 'mTORC1 promotes proliferation of immature Schwann cells and myelin growth of differentiated Schwann cells.' PNAS. 10.1073/pnas.1620761114.
Assinck P, Duncan GJ, Plemel JR, Lee MJ, Stratton JA, Manesh SB, Liu J, Ramer LM, Kang SH, Bergles DE, Biernaskie J, Tetzlaff W (2017), 'Myelinogenic Plasticity of Oligodendrocyte Precursor Cells following Spinal Cord Contusion Injury.' The Journal of Neuroscience. 10.1523/JNEUROSCI.2409-16.2017.
Larson VA, Mironova Y, Vanderpool KG, Waisman A, Rash JE, Agarwal A, Bergles DE (2018), 'Oligodendrocytes control potassium accumulation in white matter and seizure susceptibility.' eLife. 10.7554/eLife.34829.
Hines TJ, Gao X, Sahu S, Lange MM, Turner JR, Twiss JL, Smith DS (2018), 'An Essential Postdevelopmental Role for Lis1 in Mice.' eNeuro. 10.1523/ENEURO.0350-17.2018.
Yung A, Mattucci S, Bohnet B, Liu J, Fournier C, Tetzlaff W, Kozlowski P, Oxland T (2018), 'Diffusion tensor imaging shows mechanism-specific differences in injury pattern and progression in rat models of acute spinal cord injury.' NeuroImage. 10.1016/j.neuroimage.2018.10.067.
Tania M. Fowke, Robert Galinsky, Joanne O. Davidson, Guido Wassink, Rashika N. Karunasinghe, Jaya D. Prasad, Laura Bennet, Alistair J. Gunn, Justin M. Dean (2018), 'Loss of interneurons and disruption of perineuronal nets in the cerebral cortex following hypoxia-ischaemia in near-term fetal sheep.' Nature Scientific Reports. 10.1038/s41598-018-36083-y.
Xu, Zhizhonga,c,; Adilijiang, Abulimitia,c,; Wang, Wenqianga; You, Pana; Lin, Duoduoa; Li, Xinmina,c; He, Jue (2019), 'Arecoline attenuates memory impairment and demyelination in a cuprizone-induced mouse model of schizophrenia.' NeuroReport. 10.1097/WNR.0000000000001172.
Cassandra E Meyer, Josephine L Gao, James Ying-Jie Cheng, Mandavi R Oberoi, Hadley Johnsonbaugh, Stefano Lepore, Florian Kurth, Mackenzie J Thurston, Noriko Itoh, Kevin R Patel, Rhonda R Voskuhl and Allan MacKenzie-Graham (2019), 'Axonal damage in spinal cord is associated with gray matter atrophy in sensorimotor cortex in experimental autoimmune encephalomyelitis.' Multiple Sclerosis Journal. 10.1177/1352458519830614.

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Anti-Myelin Basic Protein Antibody

An antipeptide antibody was generated in chickens against a sequence shared between the mouse (NP_034907) and human (NP_002376) gene products. Antibodies were affinity-purified and the concentration adjusted to 100 µg/mL.