Anti-Myelin Basic Protein Antibody

Name: Anti-Myelin Basic Protein Antibody
Brand: Aves Labs
SKU: MBP
Price: 440.0 USD
Availability: InStock

from Aves Labs Datasheet

Trial Size

27 Citations

An antipeptide antibody was generated in chickens against a sequence shared between the mouse (NP_034907) and human (NP_002376) gene products. Antibodies were affinity-purified and the concentration adjusted to 100 µg/mL.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Format

Affinity-Purified IgY

Gene Name

MBP

Molecular Weight

Can recognize multiple bands between 14-22 kDa, corresponding to different MBP isoforms

Go to Full Product Details

Immunohistochemical staining of MBP (RED) in the hippocampal region of a transgenic adult mouse brain. This particular transgenic mouse expresses low levels of GFP autofluorescence under control of an actin promoter region. The anti-MBP antibody (Aves Labs) was used at a 1:1000 dilution. Dr. Felix Eckenstein, University of Vermont.

Adult mouse cortical cultures were stained with chicken anti-MBP antibody (Aves Labs, green, 1:1000 dilution) and rabbit anti-GFAP antibody (EnCorBio, red; 1:1000 dilution). Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.

Immunohistochemical staining of the MBP (green) in the nodes of Ranvier of the sciatic nerve of an adult rat. Red counterstaining is a ubiquitous gene product. Blue shows DAPI nuclear staining. Photomicrograph courtesy of Dr. Gerry Shaw, Univ. Florida.

Chicken anti-Myelin Basic Protein (MBP) (green), with nuclei stained with Hoechst dye (blue).

MBP: frozen sections; all washes with Triton 0.5%; Region: Striatum; Blocking: BlockHen 1%; Secondary antibody: anti-chicken FITC (Aves); Epifluorescence microscope; 40x magnification.

Immunohistochemical staining of MBP (RED) in the hippocampal region of a transgenic adult mouse brain. This particular transgenic mouse expresses low levels of GFP autofluorescence under control of an actin promoter region. The anti-MBP antibody (Aves Labs) was used at a 1:1000 dilution. Dr. Felix Eckenstein, University of Vermont.

Volume: 1000 µL

Variant

Price:

$440

Quantity:

Bulk Order Anti-Myelin Basic Protein Antibody

Product Details

Target

Myelin Basic Protein (MBP)

Target Description

Human Myelin Basic Protein (MBP) is a 20,115 dalton protein (186 amino acids) found in myelin of the CNS and PNS. Expressed as an intracellular protein by oligodendrocytes and Schwann cells, MBP aids in the compaction and stability of myelin.

Format

Affinity-Purified IgY

Concentration

100 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

MBP

Molecular Weight

Can recognize multiple bands between 14-22 kDa, corresponding to different MBP isoforms

Antigen

Synthetic peptide

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313550

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the MBP gene product shared between the human (NP_002376, NCBI) and mouse (NP_034907, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. The concentrations of the eluates were then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:1000-1:2000
IHC: 1:1000-1:2000
ICC: 1:1000-1:2000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Synonyms

Myelin basic protein (MBP) (Myelin A1 protein) (Myelin membrane encephalitogenic protein)

UniProt Details

UniProt (Immunogen Species): P02686

Product Specific References for Applications and Species

Immunocytochemistry: Rabbit | Rat
Immunohistochemistry: Mouse | Rabbit | Rat | Sheep
Western Blot: Human | Mouse
Additional Publications: Unspecified

Immunocytochemistry: Rabbit
PMID	Dilution	Publication
35343655	1:100	Liu, P., et al. 2022. A comparative study of bronchopulmonary slowly adapting receptors between rabbits and rats. Physiological reports, e15069.

Immunocytochemistry: Rat
PMID	Dilution	Publication
37272718	1:500	Nakamura, DS, et al. 2023. Expression and Subcellular Localization of Mitochondrial Docking Protein, Syntaphilin, in Oligodendrocytes and CNS Myelin Sheath. Glia, .
35343655	1:100	Liu, P., et al. 2022. A comparative study of bronchopulmonary slowly adapting receptors between rabbits and rats. Physiological reports, e15069.
32910475	not listed	Nakamura, D.S., et al. 2020. Mitochondrial Dynamics and Bioenergetics Regulated by Netrin-1 in Oligodendrocytes.. Glia, 392-412.
30937398	1:1000	Xu, Y.K.T., et al. 2019. Deep learning for high-throughput quantification of oligodendrocyte ensheathment at single-cell resolution. Communications Biology, 116.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
36343678	1:300	Ghalat, J., et al. 2023. Cis-p-tau Plays Crucial Role In Lysolecithin-Induced Demyelination And Subsequent Axonopathy In Mouse Optic Chiasm. Experimental Neurology, 114262.
36089914	1:500	Luo, W., et al. 2022. Remyelination in neuromyelitis optica spectrum disorder is promoted by edaravone through mTORC1 signaling activation. Glia, 284-304.
34362912	1:1000	Call, C.L., et al. 2021. Cortical neurons exhibit diverse myelination patterns that scale between mouse brain regions and regenerate after demyelination. Nature Communications, 4767.
31116973	1:100	Xin, W., et al. 2019. Oligodendrocytes Support Neuronal Glutamatergic Transmission via Expression of Glutamine Synthetase.. Cell Reports, 2262-2271.
30843756	1:500	Meyer, C.E., et al. 2019. Axonal damage in spinal cord is associated with gray matter atrophy in sensorimotor cortex in experimental autoimmune encephalomyelitis. Multiple Sclerosis Journal, 294-303.
30406183	1:200	Micheva, K.D., et al. 2018. Distinctive Structural and Molecular Features of Myelinated Inhibitory Axons in Human Neocortex.. eNeuro, .
29596047	1:500	Larson, V.A., et al. 2018. Oligodendrocytes control potassium accumulation in white matter and seizure susceptibility. eLife, e34829.
29404402	1:500	Hines, T.J., et al. 2018. An Essential Postdevelopmental Role for Lis1 in Mice. eNeuro, .
28821276	1:500	Santo Araújo, S.E., et al. 2017. Inflammatory demyelination alters subcortical visual circuits. Jounral of Neuroinflammation, 162.
28760862	1:500	Assinck, P., et al. 2017. Myelinogenic Plasticity of Oligodendrocyte Precursor Cells following Spinal Cord Contusion Injury. The Journal of Neuroscience, 8635-8654.
27339393	1:200	Hegde, S., et al. 2016. Phosphodiesterase 11A (PDE11A), Enriched in Ventral Hippocampus Neurons, is Required for Consolidation of Social but not Nonsocial Memories in Mice. Neuropsychopharmacology, 2920-2931.
25451251	1:1000	Yamada, H., et al. 2014. Transplantation of mature adipocyte-derived dedifferentiated fat cells promotes locomotor functional recovery by remyelination and glial scar reduction after spinal cord injury in mice. Biochemical and Biophysical Research Communications, 341-346.

Immunohistochemistry: Rabbit
PMID	Dilution	Publication
34848803	1:100	Liu, J., et al. 2021. A single baroreceptor unit consists of multiple sensors. Scientific Reports, 23111.
28018231	1:2000	Liu, J., et al. 2016. Slowly Adapting Sensory Units Have More Receptors in Large Airways than in Small Airways in Rabbits. Frontiers in Pysciology, 588.

Immunohistochemistry: Rat
PMID	Dilution	Publication
33006124	1:300	Moreno-Luna, R., et al. 2020. Heterogeneity of the Endocannabinoid System Between Cerebral Cortex and Spinal Cord Oligodendrocytes. Molecular Neurobiology, 689-702.
30409758	1:200	Yung, A., et al. 2018. Diffusion tensor imaging shows mechanism-specific differences in injury pattern and progression in rat models of acute spinal cord injury. Neuroimage, 43-55.

Immunohistochemistry: Sheep
PMID	Dilution	Publication
30523273	1:500	Fowke, T.M., et al. 2018. Loss of interneurons and disruption of perineuronal nets in the cerebral cortex following hypoxia-ischaemia in near-term fetal sheep. Scientific Reports, 17686.

Western Blot: Human
PMID	Dilution	Publication
35806400	not listed	Kamaeva, D.A., et al. 2022. Catalytic Antibodies in Bipolar Disorder: Serum IgGs Hydrolyze Myelin Basic Protein. International journal of molecular sciences, 7397.

Western Blot: Mouse
PMID	Dilution	Publication
30571667	1:2000	Xu, Z., et al. 2018. Arecoline attenuates memory impairment and demyelination in a cuprizone-induced mouse model of schizophrenia. Neuroreport, 134-138.
28484008	1:200	Beirowski, B., et al. 2017. mTORC1 promotes proliferation of immature Schwann cells and myelin growth of differentiated Schwann cells. PNAS, E4261-E4270.
28407788	not listed	Choquet, K., et al. 2017. Absence of neurological abnormalities in mice homozygous for the Polr3a G672E. Molecular Brain, 13.

Additional Publications: Unspecified
PMID	Publication
35568187	Madalena, K.M., et al. 2022. Genetic deletion of the glucocorticoid receptor in Cx3cr1+ myeloid cells is neuroprotective and improves motor recovery after spinal cord injury. Experimental neurology, 114114.

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Anti-Myelin Basic Protein Antibody

An antipeptide antibody was generated in chickens against a sequence shared between the mouse (NP_034907) and human (NP_002376) gene products. Antibodies were affinity-purified and the concentration adjusted to 100 µg/mL.