Name: Anti-Munc18-1 (Ser241) Antibody
Brand: PhosphoSolutions
SKU: p1305-241
Price: 415.0 USD
Availability: InStock

Our Anti-Munc18-1 (Ser241) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Munc18-1 (Ser241) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Mouse

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Sheep

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

STXBP1

Molecular Weight

65 kDa

Go to Full Product Details

Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~65 kDa Munc18-1 protein phosphorylated at Ser241 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Munc18-1 (Ser241) Antibody

Product Details

Target

Munc18-1 (Ser241)

Target Description

Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc18-1 is thought to be regulated by phosphorylation.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

STXBP1

Molecular Weight

65 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser241 of mouse Munc18-1, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Sheep

Antibody Registry ID

AB_2492155

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~65 kDa Munc18-1 protein phosphorylated at Ser241. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser241

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

FLJ37475 antibody, Munc 18 1 antibody, Munc 18a antibody, MUNC18 1 antibody, N-Sec1 antibody, Neuronal SEC1 antibody, NSec1 antibody, p67 antibody, Protein unc-18 homolog 1 antibody, Protein unc-18 homolog A antibody, Rb sec1 antibody, RBSEC1 antibody, STXB1_HUMAN antibody, STXBP1 antibody, Syntaxin binding protein 1 antibody, Syntaxin-binding protein 1 antibody, Unc 18 homolog antibody, Unc 18A antibody, Unc-18A antibody, Unc18 1 antibody, UNC18 antibody, Unc18-1 antibody

UniProt Details

UniProt (Human): Q15833
UniProt (Immunogen Species): O08599

NCBI Gene ID

20910

Product Specific References for Applications and Species

Immunohistochemistry: Mouse | Rat
Western Blot: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
28520937	1:500	Wang, W., et al. 2018. Atypical Endocannabinoid Signaling Initiates a New Form of Memory-Related Plasticity at a Cortical Input to Hippocampus. Cerebral Cortex, pp.1-14.

Immunohistochemistry: Rat
PMID	Dilution	Publication
28520937	1:500	Wang, W., et al. 2018. Atypical Endocannabinoid Signaling Initiates a New Form of Memory-Related Plasticity at a Cortical Input to Hippocampus. Cerebral Cortex, pp.1-14.

Western Blot: Mouse
PMID	Dilution	Publication
26407320	1:1000	Meijer, M., et al. 2015. Synaptic Effects of Munc18-1 Alternative Splicing in Excitatory Hippocampal Neurons. PloS One, 10(9), e0138950.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Munc18-1 (Ser241) Antibody

Our Anti-Munc18-1 (Ser241) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Munc18-1 (Ser241) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.