Name: Anti-Mnk2a Antibody
Brand: PhosphoSolutions
SKU: 1180-Mnk2a
Price: 415.0 USD
Availability: InStock

Our Anti-Mnk2a rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Mnk2a and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human

Expected Reactivity

Non-Human Primate

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

MKNK2

Molecular Weight

47 kDa

Go to Full Product Details

Western blot of HeLa lysate showing specific immunolabeling of the ~52 kDa Mnk2a isoform in the third lane (-). Specificity is shown in the second lane (Mnk2a) where immunolabeling is blocked by preadsorption of the Mnk2a peptide used as the antigen, but not by the Mnk2b peptide in the first lane.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Mnk2a Antibody

Product Details

Target

Mnk2a

Target Description

MAP kinase-interacting kinases Mnk1 and Mnk2 are activated by the MAP kinases, ERK and p38 (Waskiewicz et al., 1997). Additionally, Mnk1 and Mnk2 have been shown to phosphorylate the translation initiation factor eIF4E (Fukunaga and Hunter 1997; Waskiewicz et al., 1997). Alternative splicing of the MKNK2 gene results in two isoforms with different C-termini: one that has a MAPK-binding domain, Mnk2a, and one that does not, Mnk2b (Parra et al., 2005). Recent studies have indicated that Mnk2a has tumor suppressive activity while Mnk2b is pro-oncogenic (Maimon et al., 2014).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

MKNK2

Molecular Weight

47 kDa

Antigen

Synthetic peptide from the C-terminal region, specific to the human Mnk2a isoform. The sequence of the antigen has no homology with Mnk1 or Mnk2b.

Species Reactivity

Human

Expected Reactivity

Non-Human Primate

Antibody Registry ID

AB_2631038

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via chromatography on peptide immunogen column.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~52 kDa Mnk2a protein. Immunolabeling is blocked by preadsorption with the Mnk2a peptide used as antigen, but not by Mnk2b peptide.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

ADCAD1 antibody, MADS box transcription enhancer factor 2 polypeptide A (myocyte enhancer factor 2A) antibody, MEF2 antibody, MEF2A antibody, MEF2A_HUMAN antibody, Myocyte enhancer factor 2A antibody, Myocyte-specific enhancer factor 2A antibody, RSRFC4 antibody, RSRFC9 antibody, Serum response factor like protein 1 antibody, Serum response factor-like protein 1 antibody

UniProt Details

UniProt (Human): Q9HBH9
UniProt (Immunogen Species): Q9HBH9

NCBI Gene ID

2872

Product Specific Protocols

Western Blot Protocol: Download

General References

Fukunaga, R. and Hunter, T., 1997. MNK1, a new MAP kinase‐activated protein kinase, isolated by a novel expression screening method for identifying protein kinase substrates. The EMBO journal, 16(8), pp.1921-1933. PMID: 9155018
Maimon, A., Mogilevsky, M., Shilo, A., Golan-Gerstl, R., Obiedat, A., Ben-Hur, V., Lebenthal-Loinger, I., Stein, I., Reich, R., Beenstock, J. and Zehorai, E., 2014. Mnk2 alternative splicing modulates the p38-MAPK pathway and impacts Ras-induced transformation. Cell Reports, 7(2), pp.501-513. PMID: 24726367
Parra, J.L., Buxadé, M. and Proud, C.G., 2005. Features of the catalytic domains and C termini of the MAPK signal-integrating kinases Mnk1 and Mnk2 determine their differing activities and regulatory properties. Journal of Biological Chemistry, 280(45), pp.37623-37633. PMID: 16162500
Waskiewicz, A.J., Flynn, A., Proud, C.G. and Cooper, J.A., 1997. Mitogen‐activated protein kinases activate the serine/threonine kinases Mnk1 and Mnk2. The EMBO journal, 16(8), pp.1909-1920. PMID: 9155017

Recover password

Create my account

Anti-Mnk2a Antibody

Our Anti-Mnk2a rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Mnk2a and is antigen affinity purified from pooled serum. It is great for use in WB.