Our Anti-Microtubule Associated Protein 2 (MAP2) primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, human, mouse, and rat Microtubule Associated Protein 2 (MAP2) and is total IgY fraction. It is great for use in WB, IHC, ICC.
Immunofluorescence of cortical neuron-glial cell culture from E20 rat labeled with Anti- MAP2(cat. 1100-MAP2, red, 1:10,000), and Anti-Tau (green). The blue is DAPI staining of nuclear DNA. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.
Anti-Microtubule Associated Protein 2 (MAP2) Antibody
Bulk Order Anti-Microtubule Associated Protein 2 (MAP2) Antibody
Microtubule Associated Protein 2 (MAP2)
Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein τ (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006).
Total IgY fraction
ICC, IHC, WB
Recombinant bovine MAP2 protein expressed in and purified from E. Coli.
Bovine, Human, Mouse, Rat
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.