Name: Anti-Metabotropic Glutamate Receptor 7 (Ser862) Antibody
Brand: PhosphoSolutions
SKU: p1230-862
Price: 415.0 USD
Availability: InStock

Our Anti-Metabotropic Glutamate Receptor 7 (Ser862) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Metabotropic Glutamate Receptor 7 (Ser862) and is antigen affinity purified. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Human, Non-Human Primate, Zebrafish

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

GRM7

Molecular Weight

102 kDa

Go to Full Product Details

Western blot of mouse brain lysate showing the specific immunolabeling of the ~102 kDa mGluR7 protein phosphorylated at Ser862 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Metabotropic Glutamate Receptor 7 (Ser862) Antibody

Product Details

Target

Metabotropic Glutamate Receptor 7 (Ser862)

Target Description

Metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). The mGluRs are divided into three groups based on sequence identity and pharmacological properties: group I (mGluR1 and mGluR5) are localized in the perisynaptic region of the postsynaptic membrane, whereas group II (mGlur2 and mGluR3) and group III (mGluR4,6,7 and 8) are localized predominantly at presynaptic terminals. PKC phosphorylation of Ser-862 on mGluR7 has been shown to be critical for stabilizing receptor surface expression and promoting binding to the synaptic PDZ-domain-containing protein PICK1 (Suh et al., 2008).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

GRM7

Molecular Weight

102 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser862 of rat mGluR7, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Human, Non-Human Primate, Zebrafish

Antibody Registry ID

AB_2492153

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for the ~102 kDa mGluR7 protein phosphorylated at Ser862. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide used as antigen but not by the corresponding dephosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser862

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

FLJ40498 antibody, GLUR7 antibody, Glutamate receptor metabotropic 7 antibody, GPRC1G antibody, GRM7 antibody, GRM7_HUMAN antibody, Metabotropic glutamate receptor 7 antibody, MGLU7 antibody, mGluR7 antibody, OTTHUMP00000206961 antibody, OTTHUMP00000214674 antibody, OTTHUMP00000214675 antibody

UniProt Details

UniProt (Human): Q14831
UniProt (Immunogen Species): P35400

NCBI Gene ID

81672

Product Specific Protocols

Western Blot Protocol: Download

General References

Bhattacharya M, Babwah AV, Godin C, Anborgh PH, Dale LB, Poulter MO, Ferguson SSG (2004) Ral and phospholipase D2-dependent pathway for constitutive metabotropic glutamate receptor endocytosis. J Neurosci, 24:8752-8761. PMID: 15470141
Francesconi W, Cammalleri M, Sanna PP (2004) The metabotropic glutamate receptor 5 is necessary for late-phase long-term potentiation in the hippocampal CA1 region. Brain Res, 1022:12-18. PMID: 15353208
Wilson RI, Nicoll RA (2001) Endogenous cannabinoids mediate retrograde signalling at hippocampal synapses. Nature (London), 410:588-592. PMID: 11279497
Suh YH, Pelkey KA, Lavezzari G, Roche PA, Huganir RL, McBain CJ, Roche KW. (2008)Corequirement of PICK1 binding and PKC phosphorylation for stable surface expression of the metabotropic glutamate receptor mGluR7. Neuron, 58(5):736-48. PMID: 18549785

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Anti-Metabotropic Glutamate Receptor 7 (Ser862) Antibody

Our Anti-Metabotropic Glutamate Receptor 7 (Ser862) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Metabotropic Glutamate Receptor 7 (Ser862) and is antigen affinity purified. It is great for use in WB.