Name: Anti-MEK5 (Ser311, Thr315) Antibody
Brand: PhosphoSolutions
SKU: p183-3115
Price: 415.0 USD
Availability: InStock

Our Anti-MEK5 (Ser311, Thr315) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat MEK5 (Ser311, Thr315) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Mouse, Xenopus, Zebrafish

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

MAP2K5

Molecular Weight

49 kDa

Go to Full Product Details

Western blot of rat testes lysate showing specific labeling of the ~49 kDa MEK5 protein phosphorylated at Ser311 Thr315 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-MEK5 (Ser311, Thr315) Antibody

Product Details

Target

MEK5 (Ser311, Thr315)

Target Description

MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999). An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003). Dual phosphorylation of Ser-311 and Thr-315 have been implicated in cell proliferation (Cameron et al., 2004).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

MAP2K5

Molecular Weight

49 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 of rat MEK5, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Mouse, Xenopus, Zebrafish

Antibody Registry ID

AB_2492149

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~49 kDa MEK5 protein phosphorylated at Ser311, Thr315. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser311, Thr315

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Dual specificity mitogen activated protein kinase kinase 5 antibody, Dual specificity mitogen-activated protein kinase kinase 5 antibody, EC 2.7.12.2 antibody, HsT17454 antibody, MAP kinase kinase 5 antibody, MAP kinase kinase MEK5b antibody, MAP2K5 antibody, MAPK/ERK kinase 5 antibody, MAPKK 5 antibody, MAPKK5 antibody, MEK 5 antibody, mitogen-activated protein kinase kinase 5 antibody, MKK5 antibody, MP2K5_HUMAN antibody, PRKMK5 antibody, Protein kinase mitogen-activated kinase 5 antibody, SAPKK5 antibody, SKK5 antibody

UniProt Details

UniProt (Human): Q13163
UniProt (Immunogen Species): Q62862

NCBI Gene ID

31068

Product Specific Protocols

Western Blot Protocol: Download

General References

Zhou G, Bao ZQ, Dixon JE, (1995) Components of a new human protein kinase signal transduction pathway.Journal of Biological Chemistry 270(21):12665-9. PMID: 7759517
Chao TH, Hayashi M, Tapping RI, Kato Y,Lee JD (1999) MEKK3 directly regulates MEK5 activity as part of the big mitogen-activated protein kinase 1 (BMK1) signaling pathway.Journal of Biological Chemistry 274(51):36035-8. PMID: 10593883
Mehta PB, Jenkins BL, McCarthy L, Thilak L, Robson CN, Neal DE, Leung HY (2003) MEK5 overexpression is associated with metastatic prostate cancer, and stimulates proliferation, MMP-9 expression and invasion. Oncogene 22(9):1381-9.PMID: 12618764
Cameron SJ, Abe J, Malik S, Che W, Yang J (2004) Differential role of MEK5alpha and MEK5beta in BMK1/ERK5 activation. Journal of Biological Chemistry 279(2):1506-12. PMID: 14583600

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Anti-MEK5 (Ser311, Thr315) Antibody

Our Anti-MEK5 (Ser311, Thr315) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat MEK5 (Ser311, Thr315) and is antigen affinity purified from pooled serum. It is great for use in WB.