Our Anti-MEK5 (Ser311, Thr315) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat MEK5 (Ser311, Thr315) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat testes lysate showing specific labeling of the ~49 kDa MEK5 protein phosphorylated at Ser311 Thr315 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Anti-MEK5 (Ser311, Thr315) Antibody
SKU: p183-3115
Product Details
MEK5 (Ser311, Thr315)
MEK5 (also known as MKK5) is a dual specificity serine/threonine protein kinase belonging to the MAP kinase kinase family. MEK5 has been shown to specifically activate ERK5 (Zhou et al., 1995) whereas MEK5 itself is regulated by MEKK3 (Chao et al., 1999). An important link between MEK5 and metastatic prostrate cancer has been demonstrated (Mehta et.al., 2003). Dual phosphorylation of Ser-311 and Thr-315 have been implicated in cell proliferation (Cameron et al., 2004).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
MAP2K5
49 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser311/Thr315 of rat MEK5, conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for endogenous levels of the ~49 kDa MEK5 protein phosphorylated at Ser311, Thr315. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Phosphorylated
Ser311, Thr315
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Zhou G, Bao ZQ, Dixon JE, (1995) Components of a new human protein kinase signal transduction pathway.Journal of Biological Chemistry 270(21):12665-9. PMID: 7759517
Chao TH, Hayashi M, Tapping RI, Kato Y,Lee JD (1999) MEKK3 directly regulates MEK5 activity as part of the big mitogen-activated protein kinase 1 (BMK1) signaling pathway.Journal of Biological Chemistry 274(51):36035-8. PMID: 10593883
Mehta PB, Jenkins BL, McCarthy L, Thilak L, Robson CN, Neal DE, Leung HY (2003) MEK5 overexpression is associated with metastatic prostate cancer, and stimulates proliferation, MMP-9 expression and invasion. Oncogene 22(9):1381-9.PMID: 12618764
Cameron SJ, Abe J, Malik S, Che W, Yang J (2004) Differential role of MEK5alpha and MEK5beta in BMK1/ERK5 activation. Journal of Biological Chemistry 279(2):1506-12. PMID: 14583600
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