Name: Anti-MEK1 (Thr292) Antibody
Brand: PhosphoSolutions
SKU: p180-292
Price: 415.0 USD
Availability: InStock

Our Anti-MEK1 (Thr292) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse MEK1 (Thr292) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human

Expected Reactivity

Bovine, Canine, Non-Human Primate, Rat

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

MAP2K1

Molecular Weight

45 kDa

Go to Full Product Details

Western blot of recombinant wild type MEK 1 (WT) and mutant MEK 1 (T292A) (Mut). Specific immunolabeling of the ~45 kDa MEK-1 protein phosphorylated at Thr292 is shown in the second lane where MAP kinase was coexpressed.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-MEK1 (Thr292) Antibody

Product Details

Target

MEK1 (Thr292)

Target Description

MEK 1 (MAP kinase kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992). Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr-292 and Thr-386 (Mansour et al., 1994).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

MAP2K1

Molecular Weight

45 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr292 of human MEK1, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human

Expected Reactivity

Bovine, Canine, Non-Human Primate, Rat

Antibody Registry ID

AB_2492147

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~45 kDa MEK 1 protein phosphorylated at Thr292.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr292

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Dual specificity mitogen activated protein kinase kinase 1 antibody, Dual specificity mitogen-activated protein kinase kinase 1 antibody, ERK activator kinase 1 antibody, MAP kinase kinase 1 antibody, MAP kinase/Erk kinase 1 antibody, MAP2K1 antibody, MAPK/ERK kinase 1 antibody, MAPKK 1 antibody, MAPKK1 antibody, MEK 1 antibody, Mek1 antibody, MEKK1 antibody, Mitogen activated protein kinase kinase 1 antibody, MKK 1 antibody, MKK1 antibody, MP2K1_HUMAN antibody, PRKMK1 antibody, Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody, Protein kinase mitogen activated kinase 1 antibody

UniProt Details

UniProt (Human): Q02750
UniProt (Immunogen Species): Q02750

NCBI Gene ID

5604

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
25971971	not listed	Tassin, T.C., et al. 2015. Tassin, T. C., Benavides, D. R., Plattner, F., Nishi, A., & Bibb, J. A. (2015). Regulation of ERK Kinase by MEK1 Kinase Inhibition in the Brain. Journal of Biological Chemistry, Jun 26;290(26):16319-29.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-MEK1 (Thr292) Antibody

Our Anti-MEK1 (Thr292) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse MEK1 (Thr292) and is antigen affinity purified from pooled serum. It is great for use in WB.