Name: Anti-MEF2C (Ser222) Antibody
Brand: PhosphoSolutions
SKU: p1208-222
Price: 415.0 USD
Availability: InStock

Our Anti-MEF2C (Ser222) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human MEF2C (Ser222) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human

Expected Reactivity

Bovine, Chicken, Guinea Pig, Non-Human Primate, Mouse, Rat, Sheep

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

MEF2C

Molecular Weight

51 kDa

Go to Full Product Details

Western blot of OCIAML2 lysate showing specific immunolabeling of the ~51 kDa MEF2C phosphorylated at Ser222 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ((λ-Ptase, 1200 units for 30 minutes).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-MEF2C (Ser222) Antibody

Product Details

Target

MEF2C (Ser222)

Target Description

MEF2C, also known as MADS box transcription enhancer factor 2, polypeptide C, is one of 4 MEF2 (myocyte enhancer factor 2) transcription factors that encode proteins for development of skeletal muscle and brain proliferation and differentiation (McDermott et al, 1993), along with regulating stress-response during cardiac hypertrophy in mammals (Wu et al, 2015). Phosphorylation of MEF2C at serine 59 has been shown to be negatively regulated by integrin-linked kinase (ILK) (Dong et al, 2015). Recently, high MEF2C expression has been associated with a subset of acute myeloid leukemia (AML) patients with adverse-risk disease features and poor outcomes (Laszlo et al, 2015). Phosphorylation of MEF2C at serine 222 may play a key role in MEF2C signaling.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

MEF2C

Molecular Weight

51 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser222 of human MEF2C, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human

Expected Reactivity

Bovine, Chicken, Guinea Pig, Non-Human Primate, Mouse, Rat, Sheep

Antibody Registry ID

AB_2572427

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~51 kDa MEF2C protein phosphorylated at Ser222. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser222

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

C5DELq14.3 antibody, DEL5q14.3 antibody, MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) antibody, MADS box transcription enhancer factor 2 polypeptide C antibody, MEF2C antibody, MEF2C_HUMAN antibody, Myocyte enhancer factor 2C antibody, Myocyte specific enhancer factor 2C antibody, Myocyte-specific enhancer factor 2C antibody, OTTHUMP00000222409 antibody, Similar to MADS box transcription enhancer factor 2 polypeptide C antibody

UniProt Details

UniProt (Human): Q06413
UniProt (Immunogen Species): Q06413

NCBI Gene ID

4208

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
35536646	not listed	Canté-Barrett, K., et al. 2022. MEF2C Opposes Notch in Lymphoid Lineage Decision and Drives Leukemia in the Thymus. JCI Insight, e150363.
29431698	1:1000	Brown, F.C, et al. 2018. MEF2C phosphorylation is required for chemotherapy resistance in acute myeloid leukemia. Cancer Discovery, 8(4), pp.478-497.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-MEF2C (Ser222) Antibody

Our Anti-MEF2C (Ser222) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human MEF2C (Ser222) and is antigen affinity purified from pooled serum. It is great for use in WB.