Name: Anti-MARCKS Antibody
Brand: PhosphoSolutions
SKU: 1107-MARCKS
Price: 415.0 USD
Availability: InStock

Our Anti-MARCKS primary antibody from PhosphoSolutions is chicken polyclonal. It detects human MARCKS and is total IgY fraction. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Human

Expected Reactivity

Non-Human Primate

Applications

ICC, IHC, WB

Host Species

Chicken

Format

Total IgY fraction

Gene Name

MARCKS

Molecular Weight

80 kDa

Go to Full Product Details

Western blot of HeLa lysate showing specific immunolabeling of the ~80 kDa MARCKS protein.

Immunolabeling of HeLa cells showing specific labeling of the MARCKs protein (anti-MARCKS antibody, cat. 1107-MARCKS, red, 1:5000) and β-tubulin protein (green). Anti-MARCKS binds MARCKs protein expressed in the plasma membrane and cytoplasm. The blue is Hoechst staining the nuclear DNA.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-MARCKS Antibody

Product Details

Target

MARCKS

Target Description

Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al.,1990) and is concentratedin synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro (Hartwig et al. 1992). Deletion of the MARCKS gene in mice results in embryonic brain defects and death (Stumpo et al.1995). MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser-152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994).

Format

Total IgY fraction

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

MARCKS

Molecular Weight

80 kDa

Antigen

Recombinant human MARCKS expressed in and purified from E. coli

Species Reactivity

Human

Expected Reactivity

Non-Human Primate

Antibody Registry ID

AB_2751023

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Total IgY fraction

Buffer

PBS + 10 mM NaN3.

Dilution Ranges

WB: 1:10000
IHC: 1:500-1:1000
ICC: 1:500-1:5000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~80 kDa MARCKS protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

80 kDa protein antibody, 80K L antibody, 80K L protein antibody, 80K-L protein antibody, 80KL antibody,81 kDa protein, light chain antibody, light chain antibody, MACS antibody, MARCKS antibody, MARCS antibody, MARCS_HUMAN antibody, MGC52672 antibody, myristoylated alanine rich C kinase substrate antibody, Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody, Myristoylated alanine rich protein kinase C substrate antibody, Myristoylated alanine-rich C-kinase substrate antibody, Phosphomyristin antibody, PKCSL antibody, PRKCSL antibody, protein kinase C substrate 80 kDa protein light chain antibody, Protein kinase C substrate antibody

UniProt Details

UniProt (Human): P29966
UniProt (Immunogen Species): P29966

NCBI Gene ID

4082

Product Specific Protocols

Western Blot Protocol: Download

General References

Ouimet CC, Wang JKT, Walaas SI, Albert KA, Greengard P (1990) Localization of the MARCKS (87 kDa) protein, a major specific substrate for protein kinase C, in rat brain. Journal of Neuroscience 10:1683-1698. PMID: 2332803
Hartwig JH, Thelen M, Rosen A, Janmey PA, Nairn AC and Aderem A. (1992) MARCKS is an a ctin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin. Nature 356: 618-622. PMID: 1560845
Stumpo DJ, Bock CB, Tuttle JS and Blackshear PJ. (1995) MARCKS deficiency in mice leads to abnormal brain development and perinatal death. PNAS 92; 944-948. PMID: 7862670
Verghese GM, Johnson JD, Vasulka C, Haupt DM, Stumpo DJ, Blackshear PJ (1994) Protein kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein. Journal of Biological Chemistry 269:9361-9367. PMID: 8132675

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Anti-MARCKS Antibody

Our Anti-MARCKS primary antibody from PhosphoSolutions is chicken polyclonal. It detects human MARCKS and is total IgY fraction. It is great for use in WB, IHC, ICC.