Anti-Ku80 Antibody

Name: Anti-Ku80 Antibody
Brand: PhosphoSolutions
SKU: 24368
Price: 415.0 USD
Availability: InStock

from PhosphoSolutions

Our Ku80 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects hamster, human, mouse, and rat Ku80 and is antigen affinity purified. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Hamster, Human, Mouse, Rat

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

XRCC5

Go to Full Product Details

Immunoprecipitation showing detection of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto). A Ku80 deficient cell lysate, XRS, was used as a negative control.

Western Blot of 50 ug lysates of A431 (lane 1) and AR42J (lane 2) cells showing specific immunolabeling of Ku80.

Western Blot of MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954, 4T1, B16-F10, Hep3b and 293T cells lysates (20 ug/lane) showing specific immunolabeling of Ku80.

Immunoprecipitation showing detection of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto). A Ku80 deficient cell lysate, XRS, was used as a negative control.

Title: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Ku80 Antibody

Product Details

Target

Ku80

Target Description

Telomere length maintenance, an activity essential for chromosome stability and genome integrity, is regulated by telomerase- and telomere-associated factors. The DNA repair protein Ku (a heterodimer of Ku70 and Ku80 subunits) associates with mammalian telomeres and contributes to telomere maintenance. The Ku heterodimer functions at two kinds of DNA ends: telomeres and double-strand breaks. The role that Ku plays at these two classes of termini must be distinct, because Ku is required for accurate and efficient joining of double-strand breaks while similar DNA repair events are normally prohibited at chromosome ends

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

XRCC5

Antigen

Synthetic peptide corresponding to amino acids from an internal sequence of the human Ku80 protein

Species Reactivity

Hamster, Human, Mouse, Rat

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Buffer

PBS + 0.02% NaN3

Dilution Ranges

WB: 1:500-1:1000
WB Brain: 1:500-1:1000

Specificity

Reacts with the 80 kDa Ku80 protein. Sequence is 100% conserved between human, mouse, rat and hamster.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

XRCC5, X-ray repair cross-complementing protein 5, ATP-dependent DNA helicase 2 subunit 2, Ku86, CTC box-binding factor 85 kDa subunit, Lupus Ku autoantigen protein p86, Thyroid-lupus autoantigen, TLAA, G22P2

UniProt Details

UniProt (Human): P13010
UniProt (Immunogen Species): P13010

Recover password

Create my account

Anti-Ku80 Antibody

Our Ku80 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects hamster, human, mouse, and rat Ku80 and is antigen affinity purified. It is great for use in WB.