Name: Anti-Ki-67 Antibody
Brand: PhosphoSolutions
SKU: 1006-Ki67
Price: 360.0 USD
Availability: InStock

Our Anti-Ki-67 primary antibody from PhosphoSolutions is chicken polyclonal. It detects human, mouse, and rat Ki-67 and is total IgY fraction. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Format

Total IgY fraction

Gene Name

MKI67

Molecular Weight

345-395 kDa

Go to Full Product Details

Western blot of serum starved then re-fed for 2.5 hr T47D cell lysate showing specific immunolabeling of the ~345/395 Ki-67 isoforms.

Immunostaining of HeLa cells showing specific labeling of Ki-67 (cat. 1007-Ki67, 1:2000, red) present in cytoplasmic microtubules. Additional immunostaining done with β-tubulin in green and nuclear staining with DAPI (blue). During cell cycle Ki-67 protein is predominantly expressed in the nucleoli of cells during mitosis and interphase, and is not present during quiescence.

Volume: 100 µL

Variant

Price:

$360

Quantity:

Bulk Order Anti-Ki-67 Antibody

Product Details

Target

Ki-67

Target Description

Ki-67 is a nuclear protein that is considered to be essential for cellular proliferation since it is expressed only in active phases of the cell cycle (G1, S, G2 and mitosis) but not in resting phase G0 (Scholzen & Gerdes 2000). Ki-67 antibodies have therefore been widely used as markers of cell growth. Ki-67 has further been identified as a predicative and prognostic marker in breast, lung, cervical and other cancers (Yerushalmi et al., 2010; Folescu et al., 2018; Das et al., 2018).

Format

Total IgY fraction

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

MKI67

Molecular Weight

345-395 kDa

Antigen

Recombinant human ki-67 protein expressed in and purified from E. Coli.

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2737605

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Total IgY fraction

Buffer

PBS + 10 mM NaN3.

Dilution Ranges

WB: 1:5000
IHC: 1:1000-1:5000
ICC: 1:1000-1:5000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~345 & 395 kDa Ki-67 isoforms. Additional bands are thought to be breakdown products of these 2 isoforms.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Antigen identified by monoclonal antibody Ki 67 antibody, Antigen identified by monoclonal antibody Ki-67 antibody, Antigen KI-67 antibody, Antigen KI67 antibody, Antigen Ki67 antibody, Ki67 antibody, KI67_HUMAN antibody, KIA antibody, Marker of proliferation Ki-67 antibody, MIB 1 antibody, MIB antibody, MKI67 antibody, PPP1R105 antibody, Proliferation marker protein Ki-67 antibody, Proliferation related Ki 67 antigen antibody, Protein phosphatase 1 regulatory subunit 105 antibody, RP11-380J17.2 antibody

UniProt Details

UniProt (Human): P46013
UniProt (Immunogen Species): P46013

NCBI Gene ID

4288

Product Specific Protocols

Western Blot Protocol: Download

General References

Scholzen, T. and Gerdes, J., 2000. The Ki‐67 protein: from the known and the unknown. Journal of Cellular Physiology, 182(3), pp.311-322. PMID: 10653597
Yerushalmi, R., Woods, R., Ravdin, P.M., Hayes, M.M. and Gelmon, K.A., 2010. Ki67 in breast cancer: prognostic and predictive potential. The Lancet Oncology, 11(2), pp.174-183. PMID: 20152769
Folescu, R., Levai, C.M., GRIGORAŞ, M.L., Arghirescu, T.S., TALPOŞ, I.C., Gindac, C.M., ZAMFIR, C.L., Poroch, V. and Anghel, M.D., 2018. Expression and significance of Ki-67 in lung cancer. Romanian Journal of Morphology and Embryology, 59(1), pp.227-233. PMID: 29940632
Das, D., Sengupta, M., Basu, K., Tirkey, M., Datta, C. and Chatterjee, U., 2018. Role of p16/Ki-67 Dual immunostaining in detection of cervical cancer precursors. Journal of Cytology, 35(3), p.153-158. PMID: 30089944

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Anti-Ki-67 Antibody

Our Anti-Ki-67 primary antibody from PhosphoSolutions is chicken polyclonal. It detects human, mouse, and rat Ki-67 and is total IgY fraction. It is great for use in WB, IHC, ICC.