Immunostaining of HeLa cells showing specific labeling of Ki-67 in the nucleoli of rapidly dividing cells, (1:2000) in red. DNA is stained blue.
Bulk Order Anti-Ki-67 Antibody
Ki-67 is a nuclear protein that is considered to be essential for cellular proliferation since it is expressed only in active phases of the cell cycle (G1, S, G2 and mitosis) but not in resting phase G0 (Scholzen & Gerdes 2000). Ki-67 antibodies have therefore been widely used as markers of cell growth. Ki-67 has further been identified as a predicative and prognostic marker in breast, lung, cervical and other cancers (Yerushalmi et al., 2010; Folescu et al., 2018; Das et al., 2018).
Protein G Purified
Recombinant construct containing the N-terminal 300 amino acids of the human sequence expressed in and purified from E. coli.
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Protein G purified culture supernatent
PBS + 50% glycerol and 5 mm NaN3
Specific for endogenous levels of the ~345 & 395 kDa Ki-67 isoforms. Additional bands are thought to be breakdown products of these 2 isoforms.
Western blots performed on each lot
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.