Name: Anti-Insulin Receptor (Thr1160) Antibody
Brand: PhosphoSolutions
SKU: p168-1160
Price: 415.0 USD
Availability: InStock

Our Anti-Insulin Receptor (Thr1160) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human Insulin Receptor (Thr1160) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human

Expected Reactivity

Bovine, Canine, Non-Human Primate, Mouse, Rat, Xenopus

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

INSR

Molecular Weight

95 kDa

Go to Full Product Details

Western blot of HeLa cell lysate showing specific labeling of the ~95 kDa IR protein phosphorylated at Thr1160 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Insulin Receptor (Thr1160) Antibody

Product Details

Target

Insulin Receptor (Thr1160)

Target Description

The insulin receptor (IR) is a well-studied receptor tyrosine kinase composed of two α subunits, responsible for the extracellular insulin binding site, and two β subunits, responsible for intracellular protein kinase activity (Endemann et al., 1990, Chiu et al., 2010). The binding of insulin to the α subunits activates the intrinsic kinase activity located in the β subunits and subsequently initiates a cascade of phosphorylation events causing major conformational changes in the activation loop of the kinase domain, which lead to different biological functions (Chiu et al., 2010). It has been hypothesized that Thr-1160 phosphorylation affects or is affected by Tyr-1158/62/63 phosphorylation and that the conformation of Thr-1160 and pThr-1160 is likely to be affected by the phosphorylation status of the surrounding tyrosines.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

INSR

Molecular Weight

95 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr1160 of human insulin receptor, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human

Expected Reactivity

Bovine, Canine, Non-Human Primate, Mouse, Rat, Xenopus

Antibody Registry ID

AB_2492137

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~95 kDa Insulin receptor phosphorylated at Thr1160. Immunolabeling is completely eliminated with λ-phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr1160

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

CD220 antibody, HHF5 antibody, human insulin receptor antibody, Insr antibody, INSR_HUMAN antibody, Insulin receptor subunit beta antibody, IR 1 antibody, IR antibody, IR-1 antibody, IR1 antibody,

UniProt Details

UniProt (Human): P06213
UniProt (Immunogen Species): P06213

NCBI Gene ID

3643

Product Specific Protocols

Western Blot Protocol: Download

General References

Chiu SL and Cline HL (2010). Insulin receptor signaling in the development of neuronal structure and function. Neural Dev. 5:7. PMID: 17544402
Endemann G, Yonezawa K, and Roth RA (1990). Phosphatidylinositol kinase or an associated protein is a substrate for the insulin receptor tyrosine kinase. J. Biol. Chem. 265:396-400. PMID: 16038406

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Anti-Insulin Receptor (Thr1160) Antibody

Our Anti-Insulin Receptor (Thr1160) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human Insulin Receptor (Thr1160) and is antigen affinity purified from pooled serum. It is great for use in WB.