Anti-IκBα (C-terminus) Antibody

Name: Anti-IκBα (C-terminus) Antibody
Brand: PhosphoSolutions
SKU: IP1861
Price: 255.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

NFKBIA

Molecular Weight

Go to Full Product Details

Western blot image of human A431. The Blots were probed with anti-IκBα (C-term.) polyclonal antibody at a dilution of 1:500 (lane 1), 1:1000 (lane 2), and 1:2000 (lane 3).

Volume: 100 µL

Variant

Price:

$255

Quantity:

Bulk Order Anti-IκBα (C-terminus) Antibody

Product Details

Target

IκBα (C-terminus)

Target Description

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation of IκBα occurs through both serine and tyrosine phosphorylation events. Activation through phosphorylation at Ser-32 and Ser-36 is followed by proteasome-mediated degradation, resulting in the release and nuclear translocation of active NF-κB. This pathway of IκBα regulation occurs in response to various NF-κB-activating agents, such as TNFα, interleukins, LPS, and irradiation. An alternative pathway for IκBα regulation occurs through tyrosine phosphorylation of Tyr-42 and Tyr-305. Tyr-42 is phosphorylated in response to oxidative stress and growth factors. This phosphorylation can lead to degradation of IκBα and NF-κB-activation. In contrast, Tyr-305 phosphorylation by c-Abl has been implicated in IκBα nuclear translocation and inhibition of NF-κB-activation. Thus, tyrosine phosphorylation of IκBα may be an important regulatory mechanism in NF-κB signaling.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

NFKBIA

Molecular Weight

Antigen

IκBα (C-terminus) synthetic peptide (coupled to KLH) corresponding to amino acid residues at the C-terminus of human IκBα. This peptide sequence has high homology to rat and mouse IκBα, and low homology to other IκB proteins.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody detects a 38 kDa* protein on SDS-PAGE immunoblots of human A431 and Jurkat cells, as well as mouse C2C12 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

IkB, MAD3, IkappaBalpha, NFkappaB inhibitor IkBa

UniProt Details

UniProt (Human): P25963
UniProt (Immunogen Species): P25963

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Anti-IκBα (C-terminus) Antibody