Western blot image of human PC3 cells untreated (lanes 1 & 3) or treated with alkaline phosphatase to dephosphorylate histone H4 (lanes 2 and 4). The blot was probed with rabbit polyclonals anti-Histone H4 (lanes 1 & 2) and anti-Histone H4 (Tyr-72) phospho-specific antibody (lanes 3 & 4).
Bulk Order Anti-Histone H4 (Tyr-72), Phosphospecific Antibody
Histone H4 (Tyr-72)
Chromatin structure is regulated through the activity of core histones (H2A, H2B, H3, and H4) that form the nucleosome. Histone activity is regulated by a variety of post-translational modifications, including acetylation, phosphorylation, and methylation. Histone acetylation and methylation occur primarily at lysine (K) residues in the amino-terminal tail domain. These modifications are important for the regulation of histone deposition, transcriptional activation, DNA replication and repair. Acetylation and methylation of specific lysine residues creates docking sites for DNA repair, transcription, and chromatin regulatory proteins. Methylation of histones may be regulated by phosphorylation events at sites downstream of the N-terminal tail. In histone H4, both EGFR activation and inonizing radiation induce EGFR nuclear translocation and Histone H4 (Tyr-72) phosphorylation, which creates a docking site for Set8 methyltransferase. This promotes K20 methylation in Histone H4 leading to DNA synthesis and repair.
Antigen Affinity Purified
Phospho-Histone H4 (Tyr-72) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Tyr-72 in human histone H4. This site is well conserved in rat and mouse histone H4, but the site is not found in other histone family members.
Chicken, Frog, Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
The antibody was cross adsorbed to unphosphorylated histone H4 (Tyr-72) peptide before affinity purification using phospho-histone H4 (Tyr-72) peptide. This antibody antibody detects a 10 kDa* protein on SDS-PAGE immunoblots of human PC3 and A431 cells. This reactivity is not observed after alkaline phosphatase dephosphorylation of histone H4.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.