Name: Anti-HDAC2 (Ser394) Antibody
Brand: PhosphoSolutions
SKU: p1180-394
Price: 415.0 USD
Availability: InStock

Our Anti-HDAC2 (Ser394) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat HDAC2 (Ser394) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Chicken, Guinea Pig, Human, Non-Human Primate, Sheep

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

HDAC2

Molecular Weight

55 kDa

Go to Full Product Details

Western blot of mouse heart lysate showing specific immunolabeling of the ~55 kDa HDAC2 protein phosphorylated at Ser394 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-HDAC2 (Ser394) Antibody

Product Details

Target

HDAC2 (Ser394)

Target Description

Histone Deacetylase 2 (HDAC2) is part of a family of histone deacetylases that are responsible for deacetylation of lysine residues in the histone core. HDAC2 is classified as a class I histone deacetylase and is ubiquitously expressed throughout the body (Kee et al, 2008). It has been shown that HDAC2 plays an important role in cardiac hypertrophy (Eom et al, 2011). Phosphorylation of Ser-394 is responsible for the hypertrophy-associated activation of HDAC2, whereas intrinsic basal activity is maintained by phosphorylation of Ser-422 and Ser-424 (EOM et al, 2011).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

HDAC2

Molecular Weight

55 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser394 of human HDAC2, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Chicken, Guinea Pig, Human, Non-Human Primate, Sheep

Antibody Registry ID

AB_2492136

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~55 kDa HDAC2 protein phosphorylated at Ser394. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser394

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

D10Wsu179e antibody, HD 2 antibody, HD2 antibody, HDAC 2 antibody, Hdac2 antibody, HDAC2_HUMAN antibody, Histone deacetylase 2 (HD2) antibody, Histone deacetylase 2 antibody, OTTHUMP00000017046 antibody, OTTHUMP00000227077 antibody, OTTHUMP00000227078 antibody, RPD3 antibody, transcriptional regulator homolog RPD3 antibody, YAF1 antibody, YY1 associated factor 1 antibody, YY1 transcription factor binding protein antibody, Yy1bp antibody

UniProt Details

UniProt (Human): Q92769
UniProt (Immunogen Species): Q92769

NCBI Gene ID

3066

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
32138755	1:1000	Buck, J.M., et al. 2020. Developmental nicotine exposure engenders intergenerational downregulation and aberrant posttranslational modification of cardinal epigenetic factors in the frontal cortices, striata, and hippocampi of adolescent mice. Epigenetics and Chromatin, 13(1), pp.1-15.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-HDAC2 (Ser394) Antibody

Our Anti-HDAC2 (Ser394) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat HDAC2 (Ser394) and is antigen affinity purified from pooled serum. It is great for use in WB.