Our Anti-GRIP1 Associated Protein 1 (GRASP1) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat GRIP1 Associated Protein 1 (GRASP1) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat cerebellar lysate showing the specific immunolabeling of the ~95 kDa GRASP protein.
PDZ domain-containing proteins, such as PSD-95 and GRIP are thought to play key roles in glutamate receptor plasticity. GRIP-associated proteins (GRASPs) that bind to distinct PDZ domains within GRIP also play key roles in regulation of glutamate receptor function. GRASP-1 is a neuronal rasGEF associated with GRIP and AMPA receptors in vivo (Scannevin and Huganir, 2000). Recent work suggests that GRASP-1 may regulate neuronal ras signaling and contribute to the regulation of AMPA receptor distribution by NMDA receptor activity (Ye et al., 2000).
Antigen Affinity Purified from Pooled Serum
Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat GRASP1, conjugated to keyhole limpet hemocyanin (KLH).
Bovine, Canine, Human
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Prepared from pooled rabbit serum by affinity purification via chromatography on an affinity column made with the C-terminal peptide used as antigen.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB Brain: 1:1000
Specific for endogenous levels of the ~95 kDa GRASP1 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.