Anti-Green Fluorescent Protein Antibody

Name: Anti-Green Fluorescent Protein Antibody
Brand: Aves Labs
SKU: GFP-1020
Price: 485.0 USD
Availability: InStock

from Aves Labs Datasheet

747 Citations

Product Type

Primary Antibody

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Format

IgY Fraction

Molecular Weight

27 kDa

Go to Full Product Details

Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (red).

Pyramidal neurons in the hippocampal formation of a neonatal mouse brain. Tissue was paraformaldehyde-fixed (4%) and paraffin-embedded. GFP staining is in green in left panel.

Comparison between GFP immunoreactivity in Rexed Lamina 2 neurons of a transgenic mouse using Aves Labs’ anti-GFP (green) and rabbit anti-GFP (red). The transgenic mouse was generated by placing the GFP cDNA after a POMC gene promoter in the transfected plasmid. Mark Zilka, Univ. North Carolina.

Comparison between GFP-immunoreactivity using Aves' anti-GFP antibody (left panel in red) and autofluorescence (right panel in green). In this case, the cortical neuron in this unfixed thick section was first photographed for GFP autofluorescence (left), and then the section was fixed (4% paraformaldehyde) and immunostained for GFP-immunoreactivity (1:1000 dilution) using Texas Red-goat anti-chicken IgY antibodies (Jackson ImmunoResearch) as a secondary. The same cell (left) was then identified.

Immunoreactivity using anti-GFP antibody (left panel in red), Western blot showing specific immunolabeling of the GFP protein.

Immunolabeling of dividing Drosophilia larval neuroblast using Anti-GFP (green, Cat. No. GFP-1010, 1:500) to detect the GFP tagged telomere protein HOAP, and nuclei stained with DAPI (grey). Image was taken with a Zeiss 880 confocal and kindly provided by Brandt Warecki, Sullivan Lab, University of California, Santa Cruz.

Immunostaining of mouse hippocampus identifying injected HSV containing GFP (Cat GFP, 1:250, green) and DAPI (blue). Image kindly provided by Chad Brunswick, Penn State University.

Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (red).

Volume: 400 µL

Variant

Price:

$485

Quantity:

Bulk Order Anti-Green Fluorescent Protein Antibody

Product Details

Target

Green Fluorescent Protein (GFP)

Target Description

Green Fluorescent Protein (GFP) is a naturally fluorescent protein originally derived from jellyfish. GFP has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors which have become useful and ubiquitous tools in transgenic experiments. Fluorescent proteins enable a wide range of applications where they have functioned as cell lineage tracers, reporters of gene expression, or as a measure of protein-protein interactions.

Format

IgY Fraction

Concentration

10 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ELISA, ICC, IHC, WB

Host Species

Chicken

Molecular Weight

27 kDa

Antigen

Recombinant GFP expressed in Escherichia coli

Antibody Registry ID

AB_2307313

Storage

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

Physical State

Liquid

Production Notes

Chickens were immunized with purified recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks and then affinity-purified antibodies were prepared using GFP conjugated to an agarose matrix. The final product is a filter-sterilized mixture of both affinity-purified antibodies (30 µg/mL) and purified IgY (10 mg/mL).

Buffer

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.

Dilution Ranges

WB: 1:5000-1:10000
IHC: 1:2000-1:5000
ICC: 1:2000-1:5000

Quality Control

Antibodies were analyzed by western blot analysis (1:5000 dilution) and immunohistochemistry (1:500 dilution) using transgenic mice expressing the GFP gene product. Western blots were performed using BlokHen® (Aves Labs) as the blocking reagent, and HRP-labeled goat anti-chicken antibodies (Aves Labs, Cat. #H-1004) as the detection reagent. Immunohistochemistry used tetramethyl rhodamine-labeled anti-chicken IgY.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product.

Shipping

Ice packs

Product Specific References for Applications and Species

FISH
Flow Cytometry
Immunocytochemistry
Immunohistochemistry
Western Blot

FISH
PMID	Dilution	Publication
37500778	1:1000	Wang, WX, et al. 2023. Universal, label-free, single-molecule visualization of DNA origami nanodevices across biological samples using origamiFISH. Nature nanotechnology, 0.

Flow Cytometry
PMID	Dilution	Publication
34732708	1:500	Jarmas, A.E., et al. 2021. Progenitor translatome changes coordinated by Tsc1 increase perception of Wnt signals to end nephrogenesis. Nature Communications, 6332.

Immunocytochemistry
PMID	Dilution	Publication
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37329884	1:1000	Livingston, NM, et al. 2023. Bursting Translation on Single mRNAs in Live Cells. Molecular Cell, 2276-2289.
37083045	1:1000	Fan, W., et al. 2023. The Transcriptional Co-Activator Yap1 Promotes Adult Hippocampal Neural Stem Cell Activation. The EMBO Journal, e110384.
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36965484	1:500-1:1000	Tani, S., et al. 2023. Stem Cell-Based Modeling and Single-Cell Multiomics Reveal Gene-Regulatory Mechanisms Underlying Human Skeletal Development. Cell Reports, 112276.
36880745	not listed	Jansen, K.I., et al. 2023. A Live-Cell Marker to Visualize the Dynamics of Stable Microtubules Throughout the Cell Cycle. The Journal of Cell Biology, .
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36701233	1:1000	Gallagher, E.R., et al. 2023. The selective autophagy adaptor p62/SQSTM1 forms phase condensates regulated by HSP27 that facilitate the clearance of damaged lysosomes via lysophagy. Cell Reports, 112037.
36626869	1:200	Fitz, G.N., et al. 2023. Protrusion growth driven by myosin-generated force. Developmental Cell, 18-33.e6.
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36318265	1:10,000	Bernhardt, C., et al. 2022. KLF9 and KLF13 Transcription Factors Boost Myelin Gene Expression In Oligodendrocytes As Partners Of Sox10 And Myrf. Nucleic Acids Research, 11509-11528.
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Immunohistochemistry
PMID	Dilution	Publication
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37490921	1:1000	Laing, BT, et al. 2023. Anterior Hypothalamic Parvalbumin Neurons are Glutamatergic and Promote Escape Behavior. Current Biology, 3215-3228.
37463889	1:500	Kim, W, et al. 2023. An optimized Nurr1 agonist provides disease-modifying effects in Parkinson's disease models. Nature Communications, 4283.
37463577	1:600	Westerich, KJ, et al. 2023. Spatial Organization and Function of RNA Molecules within Phase-separated Condensates in Zebrafish are Controlled by Dnd1. Developmental Cell, 0.
37447268	1:1000	Iwata, S, et al. 2023. Adrenomedullin Enhances Mouse Gustatory Nerve Responses to Sugars via T1R-Independent Sweet Taste Pathway. Nutrients, 2941.
37435939	1:5000	Evans, MC, et al. 2023. Leptin, but not Estradiol, Signaling in PACAP Neurons Modulates Puberty Onset. Endocrinology, 0.
37430038	1:500	Chuapoco, MR, et al. 2023. Adeno-associated viral vectors for functional intravenous gene transfer throughout the non-human primate brain. Nature nanotechnology, 0.
37422764	1:400	Naffaa, MM, et al. 2023. Cortical Regulation of Neurogenesis and Cell Proliferation in the Ventral Subventricular Zone. Cell Reports, 112783.
37419977	1:1000	Reynolds, LM, et al. 2023. Amphetamine disrupts dopamine axon growth in adolescence by a sex-specific mechanism in mice. Nature Communications, 4035.
37390821	not listed	Wu, SJ, et al. 2023. Cortical Somatostatin Interneuron Subtypes Form Cell-type-specific Circuits. Neuron, .
37365239	1:1000	Zhao, S, et al. 2023. Chemogenetic Manipulation of CX3CR1+ Cells Transiently Induces Hypolocomotion Independent of Microglia. Molecular Psychiatry, .
37352102	not listed	Xu, J, et al. 2023. Ccdc85c-Par3 Condensates Couple Cell Polarity with Notch to Control Neural Progenitor Proliferation. Cell Reports, 112677.
37348506	1:1000	Huilgol, D, et al. 2023. Direct and Indirect Neurogenesis Generate a Mosaic of Distinct Glutamatergic Projection Neuron Types in Cerebral Cortex. Neuron, .
37333889	1:1000	Jindal, DA, et al. 2023. Early Draper-mediated Glial Refinement of Neuropil Architecture and Synapse Number in the Drosophila Antennal Lobe. Frontiers in Cellular Neuroscience, 1166199.
37332603	1:500	Hodge, R, et al. 2023. The Septate Junction Component Bark Beetle is Required for Drosophila Intestinal Barrier Function and Homeostasis. iScience, 106901.
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37217726	1:1000	Cury, KM, et al. 2023. Flexible Neural Control of Transition Points Within the Egg-laying Behavioral Sequence in Drosophila. Nature Neuroscience, 1054-1067.
37217517	1:500	Hérent, C, et al. 2023. Upregulation of Breathing Rate During Running Exercise by Central Locomotor Circuits in Mice. Nature Communications, 2939.
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37211631	1:800	Biancardi, V, et al. 2023. Cholinergic Projections to the PreBötzinger Complex. The Journal of Comparative Neurology, 1317-1332.
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37208933	1:1000	Pukos, N, et al. 2023. Chronic Demyelination and Myelin Repair After Spinal Cord Injury in Mice: A Potential Link for Glutamatergic Axon Activity. Glia, 2093-2116.
37192624	1:500	Lowenstein, ED, et al. 2023. Prox2 and Runx3 Vagal Sensory Neurons Regulate Esophageal Motility. Neuron, .
37191285	1:1000	Parab, S, et al. 2023. Local Angiogenic Interplay of Vegfc/d and Vegfa Controls Brain Region-specific Emergence of Fenestrated Capillaries. eLife, .
37172564	not listed	Schafer, ST, et al. 2023. An In vivo Neuroimmune Organoid Model to Study Human Microglia Phenotypes. Cell, 2111-2126.
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37159668	1:200	Cai, Y., et al. 2023. COMBINe Enables Automated Detection and Classification of Neurons and Astrocytes in Tissue-Cleared Mouse Brains. Cell Reports Methods, 100454.
37138703	1:500	Bise, T., et al. 2023. The Regeneration-Responsive Element Careg Monitors Activation of Müller Glia After MNU-Induced Damage of Photoreceptors in the Zebrafish Retina. Frontiers in Molecular Neuroscience, .
37119134	1:200	Cardenas-Diaz, F.L., et al. 2023. Temporal and Spatial Staging of Lung Alveolar Regeneration is Determined by the Grainyhead Transcription Factor Tfcp2l1. Cell Reports, 112451.
37117012	1:500	Li, M., et al. 2023. Hypothalamic Supramammillary Nucleus Selectively Excites Hippocampal CA3 Interneurons to Suppress CA3 Pyramidal Neuron Activity. The Journal of Neuroscience, .
37113078	1:500	Ye, F., et al. 2023. Characteristics of Activation of Monocyte-Derived Macrophages Versus Microglia After Mouse Experimental Intracerebral Hemorrhage. Journal of Cerebral Blood Flow and Metabolism, 271678X231173187.
37111695	1:1000	Hoffmann, M., et al. 2023. Smuggling on the Nanoscale-Fusogenic Liposomes Enable Efficient RNA-Transfer with Negligible Immune Response In Vitro and In Vivo. Pharmaceutics, .
37100373	1:1000	Singhal, P., et al. 2023. Characteristics of Electrical Synapses, C-terminals and Small-conductance Ca2+ activated Potassium Channel 3 (K+) Channels in the Sexually Dimorphic Cremaster Motor Nucleus in the Spinal Cord of Mouse and Rat. Neuroscience, .
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37061022	1:1000	Arshad, M.N., et al. 2023. Altered Connectomes of Adult-Born Granule Cells Following Engraftment of GABAergic Progenitors in the Mouse Hippocampus. Progress in Neurobiology, 102450.
37054704	1:1000	Sun, X.L., et al. 2023. Stem Cell Competition Driven by the Axin2-p53 Axis Controls Brain Size During Murine Development. Developmental Cell, 744-759.
37020025	1:1000	Yang, T., et al. 2023. Plastic and Stimulus-Specific Coding of Salient Events in the Central Amygdala. Nature, 510-519.
36991128	1:250	Hu, J., et al. 2023. STING Inhibits the Reactivation of Dormant Metastasis in Lung Adenocarcinoma. Nature, 806-813.
37130522	not listed	Torow, N., et al. 2023. M Cell Maturation and cDC Activation Determine the Onset of Adaptive Immune Priming in the Neonatal Peyer's Patch. Immunity, .
36972315	1:2000	Taniguchi, K., et al. 2023. Sas-Ptp10D Shapes Germ-Line Stem Cell Niche by Facilitating JNK-Mediated Apoptosis. PLoS Genetics, e1010684.
36862736	1:1000	Jones, J.D., et al. 2023. Regulation of Sleep by Cholinergic Neurons Located Outside the Central Brain in Drosophila. PLoS Biology, e3002012.
36990091	1:1000	Jung, J.H., et al. 2023. Examining the Engram Encoding Specificity Hypothesis in Mice. Neuron, .
36965484	1:500-1:1000	Tani, S., et al. 2023. Stem Cell-Based Modeling and Single-Cell Multiomics Reveal Gene-Regulatory Mechanisms Underlying Human Skeletal Development. Cell Reports, 112276.
36964750	1:5000	de Sousa, M.E., et al. 2023. Fasting and Prolonged Food Restriction Differentially Affect GH Secretion Independently of GH Receptor Signaling in AgRP Neurons. Journal of Neuroendocrinology, e13254.
36961815	1:1000	Lu, P., et al. 2023. MrgprA3-Expressing Pruriceptors Drive Pruritogen-Induced Alloknesis Through Mechanosensitive Piezo2 Channel. Cell Reports, 112283.
36949723	1:500	Viana, J.F., et al. 2023. Astrocyte Structural Heterogeneity in the Mouse Hippocampus. Glia, .
36947961	1:2000	Xie, Y., et al. 2023. New AAV Tools Fail to Detect Neurod1-Mediated Neuronal Conversion of Müller Glia and Astrocytes in Vivo. EBioMedicine, 104531.
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36912475	1:1000	Patel, A.R., et al. 2023. Neither GLP-1 Receptors nor GFRAL Neurons are Required for Aversive or Anorectic Response to DON (vomitoxin). American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, .
36894654	1:1000	Gu, X., et al. 2023. Neurons in the Caudal Ventrolateral Medulla Mediate Descending Pain Control. Nature Neuroscience, 594-605.
36890237	1:1000	Bin, N.R., et al. 2023. An Airway-to-Brain Sensory Pathway Mediates Influenza-Induced Sickness. Nature, 660-667.
36889282	1:2000	Choi, M., et al. 2023. FGF21 Counteracts Alcohol Intoxication by Activating the Noradrenergic Nervous System. Cell Metabolism, 429-437.e5.
36861441	1:400	Peng, Y.J., et al. 2023. Sertoli Cells are the Source of Stem Cell Factor for Spermatogenesis. Development, .
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36933633	1:250	Ma, R.C., et al. 2023. Origin and Diversification of Fibroblasts from the Sclerotome in Zebrafish. Developmental Biology, 35-48.
36958103	1:250	Brunswick, C.A., et al. 2023. The Clock Gene Per1 is Necessary in the Retrosplenial Cortex—but not in the Suprachiasmatic Nucleus—for Incidental Learning in Young and Aging Male Mice. Neurobiology of Aging, 77-90.
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36915679	1:200	Zhang, X., et al. 2023. Bulk and Mosaic Deletions of Egfr Reveal Regionally Defined Gliogenesis in the Developing Mouse Forebrain. iScience, 106242.
36870331	1:200	Shiraishi, K., et al. 2023. Biophysical Forces Mediated by Respiration Maintain Lung Alveolar Epithelial Cell Fate. Cell, .
36870055	1:1000	Gui, J., et al. 2023. Simultaneous Activation of Tor and Suppression of Ribosome Biogenesis by TRIM-NHL Proteins Promotes Terminal Differentiation. Cell Reports, 112181.
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36847485	1:1000	Scharbarg, E., et al. 2023. Prostaglandin D2 Controls Local Blood Flow and Sleep-Promoting Neurons in the VLPO via Astrocyte-Derived Adenosine. ACS Chemical Neuroscience, 1063-1070.
36846912	1:1000	Zuppo, D.A., et al. 2023. Foxm1 Regulates Cardiomyocyte Proliferation in Adult Zebrafish After Cardiac Injury. Development, dev201163.
36841243	1:200	Baraban, M., et al. 2023. Actomyosin Contractility in Olfactory Placode Neurons Opens the Skin Epithelium to Form the Zebrafish Nostril. Developmental Cell, 361-375.
36840844	1:500	Seeler, S., et al. 2023. A Circular RNA Expressed from the FAT3 Locus Regulates Neural Development. Molecular Neurobiology, .
36822205	1:250	Wang, C., et al. 2023. Dysregulated Lung Stroma Drives Emphysema Exacerbation by Potentiating Resident Lymphocytes to Suppress an Epithelial Stem Cell Reservoir. Immunity, 576-591.
36807972	1:600	Westerich, K.J., et al. 2023. Linking Human Dead End 1 (DND1) Variants to Male Infertility Employing Zebrafish Embryos. Human Reproduction, .
36791202	1:500	Liu, Y., et al. 2023. A Gut-Brain Axis Mediates Sodium Appetite via Gastrointestinal Peptide Regulation on a Medulla-Hypothalamic Circuit. Science Advances, eadd5330.
36780219	1:1000	Hughes, B.W., et al. 2023. NPAS4 in the Medial Prefrontal Cortex Mediates Chronic Social Defeat Stress-Induced Anhedonia-Like Behavior and Reductions in Excitatory Synapses. eLife, e75631.
36754049	1:100	Hung, S.T., et al. 2023. PIKFYVE Inhibition Mitigates Disease in Models of Diverse Forms of ALS. Cell, 786-802.
36753421	1:1000	Hattori, Y., et al. 2023. CD206+ Macrophages Transventricularly Infiltrate the Early Embryonic Cerebral Wall to Differentiate Into Microglia. Cell Reports, 112092.
36746933	1:500	Peng, W., et al. 2023. Adenosine-Independent Regulation of the Sleep-Wake Cycle by Astrocyte Activity. Cell Discovery, 16.
34820905	1:1000	Khan, A., et al. 2023. A Homozygous Missense Variant in PPP1R1B/DARPP-32 is Associated with Generalized Dystonia. Movement Disorders, 37.
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Western Blot
PMID	Dilution	Publication
37333770	1:2000	Padgett, LR, et al. 2023. Deoxyhypusine Synthase Mutations Alter the Post-translational Modification of Eukaryotic Initiation Factor 5A Resulting in Impaired Human and Mouse Neural Homeostasis. HGG Advances, 100206.
35163711	not listed	Clarke, B.E., et al. 2022. Enhanced Expression of TRAP1 Protects Mitochondrial Function in Motor Neurons under Conditions of Oxidative Stress. International Journal of Molecular Sciences, 1789.

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Anti-Green Fluorescent Protein Antibody