Name: Anti-Glycogen Synthase Kinase 3 B (GSK3B) (Ser9) Antibody
Brand: PhosphoSolutions
SKU: p1175-9
Price: 415.0 USD
Availability: InStock

Our Anti-Glycogen synthase kinase 3 B (GSK3B) (Ser9) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Glycogen synthase kinase 3 B (GSK3B) (Ser9) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Xenopus, Zebrafish

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

GSK3B

Molecular Weight

46 kDa

Go to Full Product Details

Western blot of rat cortical lysate showing specific immunolabeling of the ~46 kDa GSK3β protein phosphorylated at Ser9 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Glycogen Synthase Kinase 3 B (GSK3B) (Ser9) Antibody

Product Details

Target

Glycogen synthase kinase 3 B (GSK3B) (Ser9)

Target Description

Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase that is involved in the regulation of many signaling pathways. To date, 2 isoforms have been identified: GSK3α and GSK3β. Specifically, GSK3β has been shown to play a key inhibitory role in both the insulin and Wnt signaling pathways (Papkoff and Aikawa 1998). It has been suggested that Ser-9 phosphorylation underlies the inhibition of GSK3β by insulin (Sutherland et al., 1993).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

GSK3B

Molecular Weight

46 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 of rat GSK3β, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Xenopus, Zebrafish

Antibody Registry ID

AB_2492134

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for the ~46 kDa GSK3β protein phosphorylated at Ser9. Also weakly labels the ~51 kDa GSK3β band due to the high degree of homology between the 2 subunits. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser9

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Glycogen Synthase Kinase 3 Beta antibody, Glycogen synthase kinase-3 beta antibody, GSK 3 beta antibody, GSK-3 beta antibody, GSK3B antibody, GSK3B_HUMAN antibody, GSK3beta isoform antibody, Serine/threonine-protein kinase GSK3B antibody

UniProt Details

UniProt (Human): P49841
UniProt (Immunogen Species): P18266

NCBI Gene ID

84027

Product Specific Protocols

Western Blot Protocol: Download

General References

Sutherland C, Leighton IA, Cohen P (1993) Inactivation of glycogen synthase kinase 3 beta by phosphorylation: new kinase connections in insulin and growth factor signaling. Biochem J. Nov 15;296 (Pt. 1):15-9. PMID: 8250835
Papkoff J, Aikawa M (1998)WNT-1 and HGF regulate GSK3b activity and beta catenin signaling in mammary epithelial cells. Biochem Biophys Res Commun. Jun 29;247(3):851-8. PMID: 9647782

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Anti-Glycogen Synthase Kinase 3 B (GSK3B) (Ser9) Antibody

Our Anti-Glycogen synthase kinase 3 B (GSK3B) (Ser9) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Glycogen synthase kinase 3 B (GSK3B) (Ser9) and is antigen affinity purified from pooled serum. It is great for use in WB.