Anti-Glycogen Synthase Kinase 3 B (GSK3B) (Ser9) Antibody
Our Anti-Glycogen synthase kinase 3 B (GSK3B) (Ser9) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Glycogen synthase kinase 3 B (GSK3B) (Ser9) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat cortical lysate showing specific immunolabeling of the ~46 kDa GSK3β protein phosphorylated at Ser9 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase that is involved in the regulation of many signaling pathways. To date, 2 isoforms have been identified: GSK3α and GSK3β. Specifically, GSK3β has been shown to play a key inhibitory role in both the insulin and Wnt signaling pathways (Papkoff and Aikawa 1998). It has been suggested that Ser-9 phosphorylation underlies the inhibition of GSK3β by insulin (Sutherland et al., 1993).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
GSK3B
46 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 of rat GSK3β, conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for the ~46 kDa GSK3β protein phosphorylated at Ser9. Also weakly labels the ~51 kDa GSK3β band due to the high degree of homology between the 2 subunits. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
Phosphorylated
Ser9
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Sutherland C, Leighton IA, Cohen P (1993) Inactivation of glycogen synthase kinase 3 beta by phosphorylation: new kinase connections in insulin and growth factor signaling. Biochem J. Nov 15;296 (Pt. 1):15-9. PMID: 8250835
Papkoff J, Aikawa M (1998)WNT-1 and HGF regulate GSK3b activity and beta catenin signaling in mammary epithelial cells. Biochem Biophys Res Commun. Jun 29;247(3):851-8. PMID: 9647782
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