Our Anti-Glyceraldehyde 3-Phosphate Dehydrogenase primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Glyceraldehyde 3-Phosphate Dehydrogenase and is Protein G purified. It is great for use in WB, IHC, ICC.
Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription (1). GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease (2,3). Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic (4).
Protein G Purified
ICC, IHC, WB
Purified porcine GAPDH.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Protein G purified culture supernatant
PBS + 50% glycerol and 5 mm NaN3
WB Brain: 1:1000
Specific for endogenous levels of the ~38 kDa Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Morgenegg, G., Winkler, G.C., Hübscher, U., Heizmann, C.W., Mous, J. and Kuenzle, C.C., 1986. Glyceraldehyde‐3‐phosphate dehydrogenase is a nonhistone protein and a possible activator of transcription in neurons. Journal of Neurochemistry, 47(1), pp.54-62. PMID: 7859286
Burke, J.R., Enghild, J.J., Martin, M.E., Jou, Y.S., Myers, R.M., Roses, A.D., Vance, J.M. and Strittmatter, W.J., 1996. Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH. Nature Medicine, 2(3), p.347. PMID: 7859286
Schulze, H., Schuler, A., Stüber, D., Döbeli, H., Langen, H. and Huber, G., 1993. Rat brain glyceraldehyde‐3‐phosphate dehydrogenase interacts with the recombinant cytoplasmic domain of Alzheimer's β‐amyloid precursor protein. Journal of Neurochemistry, 60(5), pp.1915-1922. PMID: 7859286
Dastoor, Z. and Dreyer, J.L., 2001. Potential role of nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase in apoptosis and oxidative stress. Journal of Cell Science, 114(9), pp.1643-1653. PMID: 7859286
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