Name: Anti-GluR2-subunit (Ser880) Antibody
Brand: PhosphoSolutions
SKU: p1170-880
Price: 415.0 USD
Availability: InStock

Our Anti-GluR2-subunit (Ser880) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat GluR2-subunit (Ser880) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Rat

Expected Reactivity

Chicken, Human, Non-Human Primate, Mouse, Zebrafish

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

GRIA2

Molecular Weight

100 kDa

Go to Full Product Details

Western blot of rat brain lysate showing specific immunolabeling of the ~100 kDa GluR2 protein phosphorylated at Ser880 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-GluR2-subunit (Ser880) Antibody

Product Details

Target

GluR2-subunit (Ser880)

Target Description

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The number of GluR2 subunits in the AMPA receptor complex affects the Ca2+ permeability, rectification and single-channel conductance of AMPA receptors. Ser-880 has been identified as the PKC phosphorylation site within the C-terminal region of GluR2 and has been shown to differentially regulate the interaction of the PDZ domain-containing proteins GRIP1 and PICK 1 (Matsuda et al., 1999)

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

GRIA2

Molecular Weight

100 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser880 conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Rat

Expected Reactivity

Chicken, Human, Non-Human Primate, Mouse, Zebrafish

Antibody Registry ID

AB_2492131

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~100 kDa GluR2 protein phosphorylated at Ser880. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser880

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

AMPA 2 antibody, AMPA selective glutamate receptor 2 antibody, AMPA-selective glutamate receptor 2 antibody, AMPA2 antibody, GluA2 antibody, GLUR 2 antibody, GLUR B antibody, GluR K2 antibody, GluR-2 antibody, GluR-B antibody, GluR-K2 antibody, GLUR2 antibody, GLURB antibody, Glutamate receptor 2 antibody, Glutamate receptor ionotropic AMPA 2 antibody, Glutamate receptor ionotropic antibody, Gria2 antibody, GRIA2_HUMAN antibody, HBGR2 antibody

UniProt Details

UniProt (Human): P42262
UniProt (Immunogen Species): P19491

NCBI Gene ID

29627

Product Specific References for Applications and Species

Western Blot: Rat

Western Blot: Rat
PMID	Dilution	Publication
34592633	1:1000	Del Angel, Y.C., et al. 2021. Down-regulation of AMPA receptors and long-term potentiation during early epileptogenesis. Epilepsy and Behavior, 124, p. 108320.
29432883	not listed	Mao, M., et al. 2018. Regulation of Phosphorylation of AMPA Glutamate Receptors by Muscarinic M4 Receptors in the Striatum In vivo. Neuroscience, 375, pp.84-93.
28578480	not listed	Lameth, J., et al. 2017. Acute Neuroinflammation Promotes Cell Responses to 1800 MHz GSM Electromagnetic Fields in the Rat Cerebral Cortex. Neurotoxicity Research, pp.1-16.
27720500	1:2000	Singer, B.F., et al. 2016. Stimuli associated with the presence or absence of amphetamine regulate cytoskeletal signaling and behavior. European Neuropsychopharmacology, 26(11), pp.1836-1842.
26706598	not listed	O-Leary, H., et al. 2016. Enhanced long term potentiation and decreased AMPA receptor desensitization in the acute period following a single kainate induced early life seizure. Neurobiology of disease, 87, 134-144.
25689263	1:1000	Mao, L.M., et al. 2015. Dynamic increases in AMPA receptor phosphorylation in the rat hippocampus in response to amphetamine. Journal of neurochemistry, 133(6), pp.795-805.
24231469	1:1000	Xue, B., et al. 2015. Rapid and sustained GluA1 S845 phosphorylation in synaptic and extrasynaptic locations in the rat forebrain following amphetamine administration. Neurochemistry international, 64, pp.48-54.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-GluR2-subunit (Ser880) Antibody

Our Anti-GluR2-subunit (Ser880) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat GluR2-subunit (Ser880) and is antigen affinity purified from pooled serum. It is great for use in WB.