Anti-GluR1-Subunit (Ser845) Antibody
Our Anti-GluR1-Subunit (Ser845) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat GluR1-Subunit (Ser845) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser845 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Product Specific References for Applications and Species
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 35099830 | 1:100 | Sun, J., et al. 2022. LAMTOR1 inhibition of TRPML1-dependent lysosomal calcium release regulates dendritic lysosome trafficking and hippocampal neuronal function. The EMBO Journal, 41(5), p. e108119. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 37174668 | 1:100 | Hassan, Z, et al. 2023. Cognitive Impairment Is Associated with AMPAR Glutamatergic Dysfunction in a Mouse Model of Neuronal Methionine Synthase Deficiency. Cells, 1267. |
| 36565803 | not listed | Mao, L.M., et al. 2022. An Allosteric Potentiator of Metabotropic Glutamate (mGlu) 2 Receptors Reduces the Cocaine-Stimulated ERK1/2 Phosphorylation in the Mouse Striatum. Neuroscience Netters, 137028. |
| 36565803 | not listed | Mao, L.M., et al. 2022. An Allosteric Potentiator of Metabotropic Glutamate (mGlu) 2 Receptors Reduces the Cocaine-Stimulated ERK1/2 Phosphorylation in the Mouse Striatum. Neuroscience Netters, 137028. |
| 36446153 | not listed | Wei, W., et al. 2022. The Mevalonate Suppressor δ-Tocotrienol Increases AMPA Receptor-Mediated Neurotransmission. Biochemical And Biophysical Research Communications, 112-119. |
| 36351540 | 1:250 | Kuroiwa, M., et al. 2022. DARPP-32/protein phosphatase 1 regulates Rasgrp2 as a novel component of dopamine D1 receptor signaling in striatum. Neurochemistry International, 105438. |
| 34131032 | 1:250 | Sugiyama, K., et al. 2021. Subregion-Specific Regulation of Dopamine D1 Receptor Signaling in the Striatum: Implication for L-DOPA-Induced Dyskinesia. Journal of Neuroscience, 40(31), 6388-6414. |
| 33853773 | 1:1000 | Cook, S.G., et al. 2021. CaMKII holoenzyme mechanisms that govern the LTP versus LTD decision. Science Advances, 7(16), p.eabe2300. |
| 30831136 | 1:250 | Hikida, T., et al. 2019. Adolescent psychosocial stress enhances sensitization to cocaine exposure in genetically vulnerable mice. Neuroscience Research, pii: S0168-0102(19)30021-5. |
| 28613156 | 1:1000 | Musante, V., et al. 2017. Reciprocal regulation of ARPP-16 by PKA and MAST3 kinases provides a cAMP-regulated switch in protein phosphatase 2A inhibition. Elife, 6, p.e24998. |
| 28167675 | 1:1000 | Andrade, E.C., et al. 2017. ARPP-16 is a striatal-enriched inhibitor of protein phosphatase 2A regulated by microtubule-associated serine/threonine kinase 3 (Mast 3 kinase). Journal of Neuroscience, pp.4559-15. |
| 25971971 | not listed | Tassin, T.C., et al. 2015. Regulation of ERK Kinase by MEK1 Kinase Inhibition in the Brain. Journal of Biological Chemistry, Jun 26;290(26):16319-29. |
| 25649278 | not listed | Ip, F.C., et al. 2015. Anemoside A3 Enhances Cognition through the Regulation of Synaptic Function and Neuroprotection. Neuropsychopharmacology, 40, 1877–1887. |
| 25525190 | not listed | Suzuki, K., et al. 2015. In Vivo Pharmacological Characterization of TAK-063, a Potent and Selective Phosphodiesterase 10A Inhibitor with Antipsychotic-Like Activity in Rodents. Journal of Pharmacology and Experimental Therapeutics, 352:471-479. |
| 25173217 | 1:1000 | Mango, D., et al. 2014. Adenosine A1 receptor stimulation reduces D1 receptor-mediated GABAergic transmission from striato-nigral terminals and attenuates l-DOPA-induced dyskinesia in dopamine-denervated mice. Experimental Neurology, 261, pp.733-743. |
| 25136890 | 1:1000 | Squillace, M., et al. 2014. Dysfunctional dopaminergic neurotransmission in asocial BTBR mice. Translational Psychiatry, 4(8), p.e427. |
| 25115542 | not listed | Wang, J., et al. 2014. Fluoxetine Improves Behavioral Performance by Suppressing the Production of Soluble β-Amyloid in APP/PS1 Mice. Current Alzheimer Research, 11(7), 672-680. |
| 24920629 | not listed | Meyer, D.A., et al. 2014. Ischemic stroke injury is mediated by aberrant Cdk5. The Journal of Neuroscience, 34(24), 8259-8267. |
| 24907515 | not listed | Mao, L.M., et al. 2014. Propofol selectively alters GluA1 AMPA receptor phosphorylation in the hippocampus but not prefrontal cortex in young and aged mice. European Journal of Pharmacology, 738, 237-244. |
| 23303927 | not listed | Shen, Y., et al. 2013. Melanocortin-4 receptor regulates hippocampal synaptic plasticity through a protein kinase A-dependent mechanism. Journal of Neuroscience 33(2):464-72. |
| 22753408 | 1:1000 | Santini, E., et al. 2012. Dopamine- and cAMP-regulated phosphoprotein of 32-kDa (DARPP-32)-dependent activation of extracellular signal-regulated kinase (ERK) and mammalian target of rapamycin complex 1 (mTORC1) signaling in experimental parkinsonism. Journal of Biological Chemistry 287(33):27806-12. |
| 21946266 | not listed | Errico, F., et al. 2011. Higher free D-aspartate and N-methyl-D-aspartate levels prevent striatal depotentiation and anticipate L-DOPA-induced dyskinesia. Experimental Neurology, 232(2), pp.240-250. |
| 20720111 | 1:1000 | Napolitano, F., et al. 2010. Role of aberrant striatal dopamine D1 receptor/cAMP/protein kinase A/DARPP32 signaling in the paradoxical calming effect of amphetamine. Journal of Neuroscience, 30(33), pp.11043-11056. |
| 20554878 | 1:500 | Sears, R.M., et al. 2010. Regulation of Nucleus Accumbens Activity by the Hypothalamic Neuropeptide Melanin-Concentrating Hormone. Journal of Neuroscience, 30: 8263-8273. |
| 19666609 | not listed | Rebholz, H., et al. 2009. CK2 negatively regulates G s signaling. PNAS, 106: 14096-14101. |
| 19622833 | 1:750 | Santini, E., et al. 2009. Inhibition of mTOR Signaling in Parkinson’s Disease Prevents L-DOPA–Induced Dyskinesia. Science Singalling, Jul 2009; 2: ra36. |
| 19017804 | not listed | Meyer, D.A., et al. 2008. Striatal dysregulation of Cdk5 alters locomotor responses to cocaine, motor learning, and dendritic morphology. PNAS, 105: 18561 - 18566. |
| 18923023 | 1:250 | Nishi, A., et al. 2008. Distinct Roles of PDE4 and PDE10A in the Regulation of cAMP/PKA Signaling in the Striatum. Journal of Neuroscience, 28: 10460– 10471. |
| 18417701 | 1:500 | Sun, X., et al. 2008. Acute and chronic dopamine receptor stimulation modulates AMPA receptor trafficking in nucleus accumbens neurons cocultured with prefrontal cortex neurons. Journal of Neuroscience Apr 16;28(16):4216-30. |
| 17923095 | not listed | Hu,H., et al. 2007. Emotion enhances learning via norepinephrine regulation of AMPA-receptor trafficking. Cell, 131(1), pp.160-173. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 37898332 | 1:500 | Fetterly, TL, et al. 2023. Effects of junk-food on food-motivated behavior and nucleus accumbens glutamate plasticity; insights into the mechanism of calcium-permeable AMPA receptor recruitment. Neuropharmacology, 109772. |
| 35835216 | not listed | Thomas, R., et al. 2022. Integrated regulation of PKA by fast and slow neurotransmission in the nucleus accumbens controls plasticity and stress responses. The Journal of biological chemistry, 102245. |
| 34592633 | 1:1000 | Del Angel, Y.C., et al. 2021. Down-regulation of AMPA receptors and long-term potentiation during early epileptogenesis. Epilepsy and Behavior, 124, p. 108320. |
| 31994358 | 1:500 | Mao, L.M., et al. 2018. Upregulation of AMPA receptor GluA1 phosphorylation by blocking adenosine A1 receptors in the male rat forebrain. Brain and Behavior, p.e01543. |
| 29432883 | not listed | Mao, L.M., et al. 2018. Regulation of Phosphorylation of AMPA Glutamate Receptors by Muscarinic M4 Receptors in the Striatum In vivo. Neuroscience, 375, pp.84-93. |
| 29417763 | not listed | Suzuki, K., et al. 2018. Combined treatment with a selective PDE10A inhibitor TAK‐063 and either haloperidol or olanzapine at subeffective doses produces potent antipsychotic‐like effects without affecting plasma prolactin levels and cataleptic responses in rodents. Pharmacology Research & Perspectives, 6(1). |
| 29242119 | not listed | Xue, B., et al. 2017. Pharmacological modulation of AMPA receptor phosphorylation by dopamine and muscarinic receptor agents in the rat medial prefrontal cortex. European Journal of Pharmacology. 2017 Dec 11;820:45-52. |
| 28757258 | 1:1000 | Tronci, E., et al. 2017. BDNF over-expression induces striatal serotonin fiber sprouting and increases the susceptibility to l-DOPA-induced dyskinesia in 6-OHDA-lesioned rats. Experimental Neurology Nov;297:73-8. |
| 28578480 | not listed | Lameth, J., et al. 2017. Acute Neuroinflammation Promotes Cell Responses to 1800 MHz GSM Electromagnetic Fields in the Rat Cerebral Cortex. Neurotoxicity Research, pp.1-16. |
| 28167675 | 1:1000 | Andrade, E.C., et al. 2017. ARPP-16 is a striatal-enriched inhibitor of protein phosphatase 2A regulated by microtubule-associated serine/threonine kinase 3 (Mast 3 kinase). Journal of Neuroscience, pp.4559-15. |
| 27844053 | 1:5000 | Hollis, F., et al. 2016. Involvement of CRFR1 in the basolateral amygdala in the immediate fear extinction deficit. eNeuro, 3(5). |
| 27338925 | 1:1000 | Xue, B., et al. 2016. Amphetamine elevates phosphorylation of eukaryotic initiation factor 2α (eIF2α) in the rat forebrain via activating dopamine D1 and D2 receptors. Brain Research, 1646, pp.459-466. |
| 27060412 | not listed | Xue, B., et al. 2017. Integrated regulation of AMPA glutamate receptor phosphorylation in the striatum by dopamine and acetylcholine. Neuropharmacology, 112, pp.57-65. |
| 26706598 | not listed | O’Leary, H., et al. 2016. Enhanced long term potentiation and decreased AMPA receptor desensitization in the acute period following a single kainate induced early life seizure. Neurobiology of Disease, 87, 134-144. |
| 25689263 | 1:1000 | Mao, L.M., et al. 2015. Dynamic increases in AMPA receptor phosphorylation in the rat hippocampus in response to amphetamine. Journal of Neurochemistry. Jun;133(6):795-805. |
| 25525190 | not listed | Suzuki, K., et al. 2015. In Vivo Pharmacological Characterization of TAK-063, a Potent and Selective Phosphodiesterase 10A Inhibitor with Antipsychotic-Like Activity in Rodents. Journal of Pharmacology and Experimental Therapeutics, 352:471-479. |
| 24920629 | not listed | Meyer, D.A., et al. 2014. Ischemic stroke injury is mediated by aberrant Cdk5. The Journal of Neuroscience, 34(24), 8259-8267. |
| 24780380 | 1:500 | Bernard, P.B., et al. 2014. Necessary, but not sufficient: insights into the mechanisms of mGluR mediated long-term depression from a rat model of early life seizures. Neuropharmacology, 84, 1-12. |
| 24231469 | 1:1000 | Xue, B., et al. 2014. Rapid and sustained GluA1 S845 phosphorylation in synaptic and extrasynaptic locations in the rat forebrain following amphetamine administration. Neurochemistry International, 64, 48-54. |
| 23303927 | not listed | Shen, Y., et al. 2013. Melanocortin-4 receptor regulates hippocampal synaptic plasticity through a protein kinase A-dependent mechanism. Journal of Neuroscience 33(2):464-72. |
| 20554878 | 1:500 | Sears, R.M., et al. 2010. Regulation of Nucleus Accumbens Activity by the Hypothalamic Neuropeptide Melanin-Concentrating Hormone. Journal of Neuroscience, 30: 8263 - 8273. |
| 18819923 | 1:1000 | Davies, K.D., et al. 2008. Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not -Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization. Journal of Biological Chemistry, 283: 33138 - 33146. |
| 18417701 | 1:500 | Sun, X., et al. 2008. Acute and chronic dopamine receptor stimulation modulates AMPA receptor trafficking in nucleus accumbens neurons cocultured with prefrontal cortex neurons. Journal of Neuroscience Apr 16;28(16):4216-30. |
| Western Blot: Turtle | ||
| PMID | Dilution | Publication |
| 19261706 | not listed | Zheng, Z., et al. 2009. PKA Has a Critical Role in Synaptic Delivery of GluR1- and GluR4- Containing AMPARs During Initial Stages of Acquisition of In Vitro Classical Conditioning. Journal of Neurophysiology, 101: 2539 - 2549. |
Product Specific Protocols
- Western Blot Protocol: Download