Anti-GluN2B/NR2B Glutamate Receptor Antibody (N59/20)
Primary Antibody
Human, Mouse, Rat
N59/20
IHC, IP, WB
Mouse
Purified
Grin2b
166 kDa
![Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)] probed with N59/20 (left) or N52A/42 (right) TC supe.](http://www.phosphosolutions.com/cdn/shop/products/validation-image-73-097-n59-20-wb-1_{width}x.jpg?v=1626903515)
Cultured rat hippocampal neuron immunofluorescence. Image courtesy of Anthone Dunah (Harvard) and Morgan Sheng (MIT).
![Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)] probed with N59/20 (left) or N52A/42 (right) TC supe.](http://www.phosphosolutions.com/cdn/shop/products/validation-image-73-097-n59-20-wb-1_1024x.jpg?v=1626903515)
Product Specific References for Applications and Species
- Immunohistochemistry: Mouse
- Western Blot: Mouse | Rat
- Additional Publications: Unspecified
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 36672216 | 1:1000 | Ludewig, S., et al. 2023. Antibody Properties Associate with Clinical Phenotype in LGI1 Encephalitis. Cells, 282. |
| 29692709 | 1:100 | Gajardo, I., et al. 2018. Lack of pannexin 1 alters synaptic GluN2 subunit composition and spatial reversal learning in mice. Frontiers in Molecular Neuroscience, 114. |
| 24336719 | not listed | Gunn, B.G., et al. 2013. Dysfunctional astrocytic and synaptic regulation of hypothalamic glutamatergic transmission in a mouse model of early-life adversity: relevance to neurosteroids and programming of the stress response.. Journal of Neuroscience, 19534-54. |
| 23719805 | 1 : 1000 | Hoy, J.L., et al. 2013. Neuroligin1 drives synaptic and behavioral maturation through intracellular interactions.. Journal of Neuroscience, 9364-9384. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 36681359 | 1:500 | Woodburn, S.C., et al. 2023. Depletion of Microglial BDNF Increases Susceptibility to the Behavioral and Synaptic Effects of Chronic Unpredictable Stress. Brain, Behavior, and Immunity, 127-138. |
| 36417513 | not listed | Sánchez-Castillo, C., et al. 2022. Functional Specialization Of Different PI3K Isoforms For The Control Of Neuronal Architecture, Synaptic Plasticity, And Cognition. Science Advances, eabq8109. |
| 36414721 | not listed | Murtaj, V., et al. 2022. Brain Sex-Dependent Alterations After Prolonged High Fat Diet Exposure In Mice. Communications Biology, 1276. |
| 35953295 | 1:1000 | Angelini, C., et al. 2022. p140Cap Regulates the Composition and Localization of the NMDAR Complex in Synaptic Lipid Rafts. The Journal of Neuroscience, 7183-7200. |
| 34158618 | 1:500 | Seigneur, E., et al. 2021. Cerebellin-2 regulates a serotonergic dorsal raphe circuit that controls compulsive behaviors. Molecular Psychiatry, pp.1-13.. Molecular Psychiatry, 1-13. |
| 33436529 | not listed | Wang, Y.Q., et al. 2021. AMPA and NMDA receptor trafficking at cocaine-generated synapses. Journal of Neuroscience, 1996-2011. |
| 33402706 | not listed | Mossa, A., et al. 2021. Developmental impaired Akt signaling in the Shank1 and Shank3 double knock-out mice. Molecular Psychiatry, 1928-1944. |
| 33229412 | not listed | Pandian, S., et al. 2020. Myosin Va Brain-Specific Mutation Alters Mouse Behavior and Disrupts Hippocampal Synapses. eNeuro, ENEURO.0284-20.2020. |
| 32652177 | 1:1000 | Ve, H., et al. 2020. Quantitative immunoblotting analyses reveal that the abundance of actin, tubulin, synaptophysin and EEA1 proteins is altered in the brains of aged mice. Neuroscience, 100-113. |
| 32450347 | not listed | Ponzoni, L., et al. 2020. Persistent cognitive and affective alterations at late withdrawal stages after long-term intermittent exposure to tobacco smoke or electronic cigarette vapour …. Pharmacological Research, 104941. |
| 32202499 | 1:1000 | Zhang, R.S., et al. 2020. Latrophilin-2 and latrophilin-3 are redundantly essential for parallel-fiber synapse function in cerebellum. eLife, e54443. |
| 31985401 | 1:1000 | Sclip, A., et al. 2020. LAR receptor phospho-tyrosine phosphatases regulate NMDA-receptor responses. eLife, e53406. |
| 29692709 | 1:500 | Gajardo, I., et al. 2018. Lack of pannexin 1 alters synaptic GluN2 subunit composition and spatial reversal learning in mice. Frontiers in Molecular Neuroscience, 114. |
| 29069439 | 1:100 | Shahin, S., et al. 2018. From the cover: 2.45-GHz microwave radiation impairs hippocampal learning and spatial memory: Involvement of local stress mechanism-induced suppression of …. Toxicological Sciences, 349-374. |
| 28840468 | 1:400 | Sanz-Blasco, S., et al. 2018. The Kinase Fyn As a Novel Intermediate in L-DOPA-Induced Dyskinesia in Parkinson's Disease. Molecular Neurobiology, 5125-5136. |
| 26946222 | 1:1000 | Yang, P., et al. 2016. Cognitive impairments following cranial irradiation can be mitigated by treatment with a tropomyosin receptor kinase B agonist.. Experimental Neurology, 178-186. |
| 25653368 | not listed | Lo, S.C., et al. 2015. Caspase-3 deficiency results in disrupted synaptic homeostasis and impaired attention control.. Journal of Neuroscience, 2118-2132. |
| 24448406 | not listed | Li, Q., et al. 2014. The splicing regulator PTBP2 controls a program of embryonic splicing required for neuronal maturation.. eLife, e01201. |
| 23719805 | 1 : 1000 | Hoy, J.L., et al. 2013. Neuroligin1 drives synaptic and behavioral maturation through intracellular interactions.. Journal of Neuroscience, 9364-9384. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 35862168 | 1:500 | Zhou, J.J., et al. 2022. Calcineurin Controls Hypothalamic NMDA Receptor Activity and Sympathetic Outflow. Circulation Research, 345-360. |
| 33184769 | 1:250 | Barhorst, K.A., et al. 2020. Remote and Persistent Alterations in Glutamate Receptor Subunit Composition Induced by Spreading Depolarizations in Rat Brain. Cellular and Molecular Neurobiology, 1253-1260. |
| 31439005 | 1:125 | Dosemeci, A., et al. 2019. Postsynaptic densities fragment into subcomplexes upon sonication. Molecular Brain, 72. |
| 29496640 | not listed | Ji, Y., et al. 2018. Opposing roles of estradiol and testosterone on stress-induced visceral hypersensitivity in rats. The Journal of Pain, 764-776. |
| 29490268 | 1:1000 | Chen, J., et al. 2018. The α2δ-1-NMDA Receptor Complex Is Critically Involved in Neuropathic Pain Development and Gabapentin Therapeutic Actions. . Cell Reports, 2307–2321. |
| 27271196 | 1:1000 | Gross, G.G., et al. 2016. An E3-ligase-based method for ablating inhibitory synapses. Nature Methods, 673-678. |
| 25009255 | 1:200 | Kehoe, L.A., et al. 2014. GluN3A promotes dendritic spine pruning and destabilization during postnatal development.. Journal of Neuroscience, 9213-9221. |
| 24103031 | 1:500 | Luo, P., et al. 2013. Downregulation of postsynaptic density-95-interacting regulator of spine morphogenesis reduces glutamate-induced excitotoxicity by differentially regulating glutamate receptors in rat cortical neurons.. FEBS Journal, 6114-27. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 26627310 | Berg, J.M., et al. 2015. JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse.. Neuron, 1173-1191. | |
| 26557018 | Kim, S., et al. 2015. Mind Bomb-2 Regulates Hippocampus-dependent Memory Formation and Synaptic Plasticity.. The Korean Journal of Physiology and Pharmacology, 515-522. | |
| 26203142 | Zollinger, D.R., et al. 2015. The Polarity Protein Pals1 Regulates Radial Sorting of Axons.. Journal of Neuroscience, 10474-10484. | |
| 25755642 | McGuier, N.S., et al. 2015. Homer2 deletion alters dendritic spine morphology but not alcohol-associated adaptations in GluN2B-containing N-methyl-D-aspartate receptors in the nucleus accumbens.. Frontiers in Pharmacology, 28. | |
| 25707359 | Chen, N., et al. 2015. Interaction proteomics of canonical Caspr2 (CNTNAP2) reveals the presence of two Caspr2 isoforms with overlapping interactomes.. Biochimica et Biophysica Acta, 827-833. | |
| 25698751 | Chassefeyre, R., et al. 2015. Regulation of postsynaptic function by the dementia-related ESCRT-III subunit CHMP2B.. Journal of Neuroscience, 3155-3173. | |
| 25632145 | D'Orsi, B., et al. 2015. Bax regulates neuronal Ca2+ homeostasis.. Journal of Neuroscience, 1706-1722. | |
| 25513972 | Neuner, S.M., et al. 2015. TRPC3 channels critically regulate hippocampal excitability and contextual fear memory.. Behavioural Brain Research, 69-77. | |
| 25468278 | McGuier, N.S., et al. 2015. Withdrawal from chronic intermittent alcohol exposure increases dendritic spine density in the lateral orbitofrontal cortex of mice.. Alcohol, 21-27. | |
| 25319628 | Mellone, M., et al. 2015. Zinc transporter-1: a novel NMDA receptor-binding protein at the postsynaptic density.. Journal of Neurochemistry, 159-168. | |
| 25069643 | Bhattacharyya, M., et al. 2015. NMDA glutamate receptor NR1, NR2A and NR2B expression and NR2B Tyr-1472 phosphorylation in the lens.. Neurochemical Research, 1825-1832. | |
| 24728870 | Neumann, J.R., et al. 2015. Y-P30 promotes axonal growth by stabilizing growth cones.. Brain Structure and Function, 1935-1950. | |
| 25505316 | Stroebel, D., et al. 2014. Controlling NMDA receptor subunit composition using ectopic retention signals.. Journal of Neuroscience, 16630-16636. | |
| 25352748 | de Andrade, G.B., et al. 2014. Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina.. Molecular Vision, 1422-1433. | |
| 24599469 | Trantham-Davidson, H., et al. 2014. Chronic alcohol disrupts dopamine receptor activity and the cognitive function of the medial prefrontal cortex.. Journal of Neuroscience, 3706-3718. | |
| 24403152 | Portugal, G.S., et al. 2014. Hippocampal long-term potentiation is disrupted during expression and extinction but is restored after reinstatement of morphine place preference.. Journal of Neuroscience, 527-538. | |
| 24297929 | Fiuza, M., et al. 2013. GluN3A expression restricts spine maturation via inhibition of GIT1/Rac1 signaling.. Proceedings of the National Academy of Science of the United States of America, 20807-20812. | |
| 24260577 | Kunda, S., et al. 2013. Antagonist properties of Conus parius peptides on N-methyl-D-aspartate receptors and their effects on CREB signaling.. PLoS One, e81405. | |
| 24259588 | Louveau, A., et al. 2013. Impaired Spatial Memory in Mice Lacking CD3ζ Is Associated with Altered NMDA and AMPA Receptors Signaling Independent of T-Cell Deficiency. Journal of Neuroscience, 18672-18685. | |
| 24244696 | den Hartog, C.R., et al. 2013. Alterations in ethanol-induced behaviors and consumption in knock-in mice expressing ethanol-resistant NMDA receptors.. PLoS One, e80541. | |
| 24183704 | Yuan, T., et al. 2013. Expression of cocaine-evoked synaptic plasticity by GluN3A-containing NMDA receptors.. Neuron, 1025-1038. | |
| 24069373 | Breiderhoff, T., et al. 2013. Sortilin-related receptor SORCS3 is a postsynaptic modulator of synaptic depression and fear extinction.. PLoS One, e75006. | |
| 24068818 | Krüger, J.M., et al. 2013. Differential roles of postsynaptic density-93 isoforms in regulating synaptic transmission.. Journal of Neuroscience, 15504-15517. | |
| 24060584 | Viviani, B., et al. 2013. Early maternal deprivation immunologically primes hippocampal synapses by redistributing interleukin-1 receptor type I in a sex dependent manner.. Brain, Behavior, and Immunity, 135-143. | |
| 24056996 | Barcomb, K., et al. 2013. Enzymatic activity of CaMKII is not required for its interaction with the glutamate receptor subunit GluN2B.. Molecular Pharmacology, 834-843. | |
| 24032518 | Gaier, E.D., et al. 2013. Peptidylglycine α-amidating monooxygenase heterozygosity alters brain copper handling with region specificity.. Journal of Neurochemistry, 605-619. | |
| 23946397 | Bonnet, S.A., et al. 2013. Synaptic state-dependent functional interplay between postsynaptic density-95 and synapse-associated protein 102.. Journal of Neuroscience, 13398-13409. | |
| 23874151 | Frühbeis, C., et al. 2013. Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication.. PLoS Biology, e1001604. | |
| 23852340 | Marco, S., et al. 2013. Suppressing aberrant GluN3A expression rescues synaptic and behavioral impairments in Huntington''s disease models.. Nature Medicine, 1030-1038. | |
| 23831965 | Brigman, J.L., et al. 2013. GluN2B in corticostriatal circuits governs choice learning and choice shifting.. Nature Neuroscience, 1101-1110. | |
| 23486974 | Murata, Y., et al. 2013. Postsynaptic density scaffold SAP102 regulates cortical synapse development through EphB and PAK signaling pathway.. Journal of Neuroscience, 5040-5052. | |
| 23447623 | Chowdhury, D., et al. 2013. Tyrosine phosphorylation regulates the endocytosis and surface expression of GluN3A-containing NMDA receptors.. Journal of Neuroscience, 4151-4164. | |
| 23295855 | Di Filippo, M., et al. 2013. Effects of central and peripheral inflammation on hippocampal synaptic plasticity.. Neurobiology of Disease, 229-236. | |
| 22784608 | Xiao, X., et al. 2013. Estrogen in the anterior cingulate cortex contributes to pain-related aversion.. Cerebral Cortex, 2190-2203. | |
| 23223310 | Bagetta, V., et al. 2012. Rebalance of striatal NMDA/AMPA receptor ratio underlies the reduced emergence of dyskinesia during D2-like dopamine agonist treatment in experimental Parkinson''s disease.. Journal of Neuroscience, 17921-17931. | |
| 23112146 | Harraz, M.M., et al. 2012. MicroRNA-223 is neuroprotective by targeting glutamate receptors.. Proceedings of the National Academy of Science of the United States of America, 18962-18967. | |
| 22989522 | Mazzone, C.M., et al. 2012. Analysis of kalirin-7 knockout mice reveals different effects in female mice.. Molecular Pharmacology, 1241-1249. | |
| 22869379 | Zhang, Y., et al. 2012. Estrogen facilitates spinal cord synaptic transmission via membrane-bound estrogen receptors: implications for pain hypersensitivity.. The Journal of Biological Chemistry, 33268-33281. | |
| 22703994 | Mulholland, P.J., et al. 2012. Chronic ethanol up-regulates the synaptic expression of the nuclear translational regulatory protein AIDA-1 in primary hippocampal neurons.. Alcohol, 569-576. | |
| 22666364 | Kroener, S., et al. 2012. Chronic Alcohol Exposure Alters Behavioral and Synaptic Plasticity of the Rodent Prefrontal Cortex. PLoS One, e37541. | |
| 22369234 | Wilke, S.A., et al. 2012. NeuroD2 regulates the development of hippocampal mossy fiber synapses.. Neural Development, 9. | |
| 22153375 | Wang, C.C., et al. 2011. A critical role for GluN2B-containing NMDA receptors in cortical development and function.. Neuron, 789-805. | |
| 21985178 | Peng, X., et al. 2011. Neuronal Nogo-A regulates glutamate receptor subunit expression in hippocampal neurons.. Journal of Neurochemistry, 1183-1193. | |
| 21880917 | Kiraly, D.D., et al. 2011. Kalirin binds the NR2B subunit of the NMDA receptor, altering its synaptic localization and function.. Journal of Neuroscience, 12554-12565. | |
| 21708909 | Eipper-Mains, J.E., et al. 2011. microRNA-Seq reveals cocaine-regulated expression of striatal microRNAs.. RNA., 1529-1543. | |
| 21397592 | Doherty, F.C., et al. 2011. NMDA receptor subunit expression in the supraoptic nucleus of adult rats: dominance of NR2B and NR2D.. Brain Research, 89-99. | |
| 21329509 | Mains, R.E., et al. 2011. Kalrn promoter usage and isoform expression respond to chronic cocaine exposure.. BMC Neuroscience, 20. | |
| 21135233 | Fourgeaud, L., et al. 2010. MHC class I modulates NMDA receptor function and AMPA receptor trafficking.. Proceedings of the National Academy of Science of the United States of America, 22278-22283. | |
| 20826662 | Hsin, H., et al. 2010. Proline-rich tyrosine kinase 2 regulates hippocampal long-term depression.. Journal of Neuroscience, 11983–11993. | |
| 20678574 | Antion, M.D., et al. 2010. Ephrin-B3 regulates glutamate receptor signaling at hippocampal synapses.. Molecular and Cellular Neuroscience, 378-388. | |
| 20220006 | Perez de Arce, K., et al. 2010. Synaptic clustering of PSD-95 is regulated by c-Abl through tyrosine phosphorylation.. Journal of Neuroscience, 3728-3738. | |
| 20159450 | Edbauer, D., et al. 2010. Regulation of synaptic structure and function by FMRP-associated microRNAs miR-125b and miR-132.. Neuron, 373-384. | |
| 19605640 | Longordo, F., et al. 2009. NR2A at CA1 synapses is obligatory for the susceptibility of hippocampal plasticity to sleep loss.. Journal of Neuroscience, 9026-9041. | |
| 19135470 | Ghasemzadeh, M.B., et al. 2009. Locomotor sensitization to cocaine is associated with distinct pattern of glutamate receptor trafficking to the postsynaptic density in prefrontal cortex: early versus late withdrawal effects.. Pharmacology Biochemistry Behavior, 383-392. | |
| 19105975 | Ghasemzadeh, M.B., et al. 2009. Behavioral sensitization to cocaine is associated with increased glutamate receptor trafficking to the postsynaptic density after extended withdrawal period.. Neuroscience, 414-426. | |
| 19020030 | Ma, X.M., et al. 2008. Kalirin-7 is required for synaptic structure and function.. Journal of Neuroscience, 12368-12382. | |