Anti-Girdin (Tyr-1764), Phosphospecific Antibody

Name: Anti-Girdin (Tyr-1764), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: GP5801
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

1 Citation

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

CCDC88A

Molecular Weight

250

Go to Full Product Details

Western blot image of human A431 cell lysates treated with pervanadate (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Tyr-1764), phospho-specific (lanes 3 & 4).

Volume: 100 µL

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$309

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Bulk Order Anti-Girdin (Tyr-1764), Phosphospecific Antibody

Product Details

Target

Girdin (Tyr-1764)

Target Description

Girdin, a member of the CCDC88 (Hook related protein) family, is an actin binding protein involved with cell migration and maintaining cytoskeletal organization. Girdin has conserved domains at the N- and C-terminus that bind microtubules and actin, respectively. It enhances PI3-kinase dependent phosphorylation of proteins, most notably Akt. This same activity can contribute to tumor proliferation, invasion, and metastasis in breast, ovarian, prostate, and pancreatic tissues. Girdin is phosphorylated at three separate locations: Ser-1416, Ser-1674, and Tyr-1764. Ser-1416 is the primary Akt phosphorylation site, while Cyclin-dependent kinases interact with Girdin and phosphorylate Ser-1674. Multiple receptor tyrosine kinases can bind girdin and phosphorylate Tyr-1764.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

CCDC88A

Molecular Weight

250

Antigen

Phospho-Girdin (Tyr-1764) synthetic peptide (coupled to carrier) corresponding to amino acid residues around tyrosine 1764 in human Girdin. This sequence has high homology to rat and mouse Girdin, and the site is not conserved in other Girdin family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody was cross-absorbed to unphosphorylated Girdin (Tyr-1764) peptide before affinity purification using phospho-Girdin (Tyr-1764) peptide. This antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of human A431 cells stimulated with EGF and Pervanadate. These reactivities are not observed after alkaline phosphatase treatment to dephosphorylate Girdin.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-1764

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

APE, Galpha, vesicle, GIV, Girders actin filament, HkRP1, GRDN, CCDC88A

UniProt Details

UniProt (Human): Q3V6T2
UniProt (Immunogen Species): Q3V6T2

Product Specific References for Applications and Species

Immunocytochemistry: Human
Western Blot: Human

Immunocytochemistry: Human
PMID	Dilution	Publication
37503752	not listed	Hayashi, Y, et al. 2023. Girdin Regulates Both Migration and Angiogenesis in Pancreatic Cancer Cell Lines. Oncology Reports, .

Western Blot: Human
PMID	Dilution	Publication
37503752	1:500	Hayashi, Y, et al. 2023. Girdin regulates both migration and angiogenesis in pancreatic cancer cell lines. Oncology reports, 169.

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Anti-Girdin (Tyr-1764), Phosphospecific Antibody