Anti-GFAP Antibody (N206A/8)
Primary Antibody
Human, Mouse, Rat
N206A/8
ICC, IHC, WB
Mouse
Purified
GFAP
50 kDa
Adult WT and KO mouse hippocampus immunofluorescence with N206A/8 (green) and KC rabbit anti- Kv2.1 (red). Brain samples courtesy of Albee Messing, University of Wisconsin at Madison.
Product Specific References for Applications and Species
- Immunocytochemistry: Human | Mouse | Rat
- Immunohistochemistry: Drosophila | Guinea Pig | Human | Mouse | Non-Human Primate | Polar Bear | Rat
- Western Blot: Human | Mouse | Rat
- Additional Publications: Unspecified
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 35701597 | 1:1000 | Liu, D., et al. 2022. Impact of Schizophrenia GWAS Loci Converge Onto Distinct Pathways in Cortical Interneurons vs Glutamatergic Neurons During Development. Molecular Psychiatry, . |
| 33287888 | not listed | Mou, Y., et al. 2020. Impaired lipid metabolism in astrocytes underlies degeneration of cortical projection neurons in hereditary spastic paraplegia. Journal Acta Neuropathologica Communications, . |
| 33097921 | 1:1000 | Park, G.H., et al. 2020. Activated microglia cause metabolic disruptions in developmental cortical interneurons that persist in interneurons from individuals with schizophrenia. Nature Neuroscience, . |
| 33097921 | 1:1000 | Park, G.H., et al. 2020. Activated microglia cause metabolic disruptions in developmental cortical interneurons that persist in interneurons from individuals with schizophrenia. Nature Neuroscience, 1352-1364. |
| 32202516 | not listed | Boisvert, E.M., et al. 2020. A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells. Journal of visualized experiments, 10.3791/60379. |
| 29950669 | 1:500 | Park, J., et al. 2018. A 3D human triculture system modeling neurodegeneration and neuroinflammation in Alzheimer's disease.. Nat Neurosci, 941-951. |
| 27110916 | 1:100 | Xue, Y., et al. 2016. Sequential regulatory loops as key gatekeepers for neuronal reprogramming in human cells.. Nature Neuroscience, 807-15. |
| 24419315 | 1:500 | Williams, E.C., et al. 2014. Mutant astrocytes differentiated from Rett syndrome patients-specific iPSCs have adverse effects on wild-type neurons.. Hum Mol Genet., 2968-80. |
| 23313552 | 1:100 | Xue, Y., et al. 2013. Direct conversion of fibroblasts to neurons by reprogramming PTB-regulated microRNA circuits.. Cell., 82-96. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 34531281 | 1:1000 | Griggs, R.B., et al. 2021. The type 2 diabetes factor methylglyoxal mediates axon initial segment shortening and alters neuronal function at the cellular and network levels.. Eneuro, ENEURO.0201-21.2021. |
| 33767611 | 1:1000 | Liakath-Ali, K., et al. 2021. The Perils of Navigating Activity-Dependent Alternative Splicing of Neurexins.. Front Mol Neurosci, 659681. |
| 32639590 | 1:300 | Padmashri, R., et al. 2021. Modeling human‐specific interlaminar astrocytes in the mouse cerebral cortex. Journal of Comparative Neurology, . |
| 32783930 | 1:1000 | Garay, P.M., et al. 2020. RAI1 Regulates Activity-Dependent Nascent Transcription and Synaptic Scaling. Cell Reports, 108002. |
| 30680309 | 1:1000 | Chander, P., et al. 2019. Neuron-Specific Gene 2 (NSG2) Encodes an AMPA Receptor Interacting Protein That Modulates Excitatory Neurotransmission. eNeuro, ENEURO.0292-18.2018. |
| 26883496 | not listed | Zhang, W., et al. 2016. MiRNA-128 regulates the proliferation and neurogenesis of neural precursors by targeting PCM1 in the developing cortex.. Elife., . |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 31682945 | 1:300 | Dragić, M., et al. 2019. Two Distinct Hippocampal Astrocyte Morphotypes Reveal Subfield-Different Fate during Neurodegeneration Induced by Trimethyltin Intoxication. Neurosciecne, 38-54. |
| Immunohistochemistry: Drosophila | ||
| PMID | Dilution | Publication |
| 35105675 | 1:500 | Wang, L., et al. 2022. Anastasis drives senescence and non-cell autonomous neurodegeneration in the astrogliopathy Alexander disease.. Journal of Neuroscience, 2584-2597. |
| 29765022 | 1:500 | Wang, L., et al. 2018. Tissue and cellular rigidity and mechanosensitive signaling activation in Alexander disease. Nature communications, 1899. |
| Immunohistochemistry: Guinea Pig | ||
| PMID | Dilution | Publication |
| 34530852 | 1:200 | Proskocil, B., et al. 2021. TLR7 is expressed by support cells, but not sensory neurons, in ganglia.. Journal of NeuroInflamation, 209. |
| Immunohistochemistry: Human | ||
| PMID | Dilution | Publication |
| 35105675 | 1:500 | Wang, L., et al. 2022. Anastasis drives senescence and non-cell autonomous neurodegeneration in the astrogliopathy Alexander disease.. Journal of Neuroscience, 2584-2597. |
| 30975982 | 1:1000 | Boisvert, E.M., et al. 2019. Minocycline mitigates the effect of neonatal hypoxic insult on human brain organoids. Cell death and disease, 325. |
| 25864570 | 1:500 | Ristić, A.J., et al. 2015. Hippocampal antioxidative system in mesial temporal lobe epilepsy.. Epilepsia., 789-99. |
| 29765022 | 1:500 | Wang, L., et al. 2018. Tissue and cellular rigidity and mechanosensitive signaling activation in Alexander disease.. Nature Communications, 1899. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 36336067 | 1:100 | New, L.E., et al. 2022. GABAergic Regulation Of Cell Proliferation Within The Adult Mouse Spinal Cord. Neuropharmacology, 109326. |
| 36220889 | not listed | He, L., et al. 2022. C9orf72 Functions in the Nucleus to Regulate DNA Damage Repair. Cell Death and Differentiation, . |
| 35972937 | 1:250 | Pohlentz, M.S., et al. 2022. Characterisation of NLRP3 Pathway-Related Neuroinflammation in Temporal Lobe Epilepsy. PloS One, e0271995. |
| 34231259 | 1:500 | Simankova, A., et al. 2021. Ddx20, DEAD box helicase 20, is essential for the differentiation of oligodendrocyte and maintenance of myelin gene expression. Glia, 2559-2574. |
| 35121636 | 1:25 | Matsuura, K., et al. 2022. SIPA1L1/SPAR1 interacts with the neurabin family of proteins and is involved in GPCR signaling. The Journal of neuroscience : the official journal of the Society for Neuroscience, . |
| 34911751 | not listed | Kim, E.C., et al. 2021. Spontaneous seizure and memory loss in mice expressing an epileptic encephalopathy variant in the calmodulin-binding domain of Kv7.2. Proceedings of the National Academy of Sciences of the United States of America, . |
| 34617601 | 1:500 | Vaasjo, L.O., et al. 2021. Characterization and manipulation of Corticothalamic neurons in associative cortices using Syt6-Cre transgenic mice. Journal of Comparative Neurology, 44590. |
| 34231259 | 1:500 | Simankova, A., et al. 2021. Ddx20, DEAD box helicase 20, is essential for the differentiation of oligodendrocyte and maintenance of myelin gene expression. Glia, 2559-2574. |
| 32574593 | 1:1000 | Poulin, J.F., et al. 2020. PRISM: A Progenitor-Restricted Intersectional Fate Mapping Approach Redefines Forebrain Lineages. Developmental Cell, 740-753.e3.. |
| 32249896 | not listed | Sullivan, C.S., et al. 2020. Developmental Regulation of Basket Interneuron Synapses and Behavior through NCAM in Mouse Prefrontal Cortex. Cerebral Cortex, 4689-4707. |
| 32033960 | 1:500 | Siehr, M.S., et al. 2020. Arx expansion mutation perturbs cortical development by augmenting apoptosis without activating innate immunity in a mouse model of X-linked infantile spasms syndrome. Disease models and mechanisms, dmm042515. |
| 29765022 | 1:500 | Wang, L., et al. 2018. Tissue and cellular rigidity and mechanosensitive signaling activation in Alexander disease.. Nature Communications, 1899. |
| 29665166 | 1:300 | Most, D., et al. 2020. Silencing Synaptic MicroRNA-411 Reduces Voluntary Alcohol Consumption in Mice. Addiction Biology, 604-616. |
| 31597963 | 1:250 | Piatkevich, K.D., et al. 2019. Population imaging of neural activity in awake behaving mice.. Nature, 413-417. |
| 31207335 | 1:500 | olde Heuvel, F., et al. 2019. STAT6 mediates the effect of ethanol on neuroinflammatory response in TBI. Brain Behavior and Immunity, 228-246. |
| 30771034 | 1:50 | Gatto, R.G., et al. 2019. Detection of axonal degeneration in a mouse model of Huntington's disease: comparison between diffusion tensor imaging and anomalous diffusion metrics.. Magnetic Resonance Materials in Physics, Biology and Medicine, 461–471. |
| 29361558 | 1:2 (supe) | Fairchild, C.L., et al. 2018. RBX2 maintains final retinal cell position in a DAB1-dependent and -independent fashion. Development, dev155283. |
| 29343209 | 1:250 | Hansen, K.R., et al. 2018. Mild Blast Injury Produces Acute Changes in Basal Intracellular Calcium Levels and Activity Patterns in Mouse Hippocampal Neurons. Journal of Neurotrauma, 1523-1536. |
| 29306441 | 1:2000 | Du, Y., et al. 2018. Liposome-Assisted Metabolic Glycan Labeling With Cell and Tissue Selectivity. Methods in Enzymology, 321-353. |
| 29133177 | 1:100 | DeWalt, G.J., et al. 2017. Region-specific alterations in astrocyte and microglia morphology following exposure to blasts in the mouse hippocampus. Neuroscience Letters, 160-166. |
| 28614186 | 1:500 | Gandla, J., et al. 2017. miR-34c-5p functions as pronociceptive microRNA in cancer pain by targeting Cav2.3 containing calcium channels. Pain, 1765-1779. |
| 27759100 | 1:500 | Ehmsen, J.T., et al. 2016. The astrocytic transporter SLC7A10 (Asc-1) mediates glycinergic inhibition of spinal cord motor neurons.. Scientific reports, 35592. |
| 27699209 | not listed | Ueki, Y., et al. 2016. Loss of Ikbkap causes slow, progressive retinal degeneration in a mouse model of familial dysautonomia.. Eneuro, 5. |
| 27251629 | 1:100 | Fan, F., et al. 2016. Dynamin 1-and 3-mediated endocytosis is essential for the development of a large central synapse in vivo.. Jounal of Neuroscience, 6097-6115. |
| 27109181 | not listed | Marin, M.A., et al. 2016. Amyloid-β plaques disrupt axon initial segments. Experimental Neurology, 93-98. |
| 26843629 | 1:1000 | Wang, L., et al. 2016. An In Vivo Pharmacological Screen Identifies Cholinergic Signaling as a Therapeutic Target in Glial-Based Nervous System Disease.. J Neurosci., 1445-55. |
| 26799938 | 1:100 | Lee, S.A., et al. 2016. Gradient index microlens implanted in prefrontal cortex of mouse does not affect behavioral test performance over time. . PloS one, e0146533.. |
| 26516618 | not listed | Pacey, L.K., et al. 2015. Persistent astrocyte activation in the fragile X mouse cerebellum.. Brain Behav., e00400. |
| 26348152 | 1:2000 | Jiang, W., et al. 2015. An optimized method for high-titer lentivirus preparations without ultracentrifugation.. Sci Rep., 13875. |
| 26038197 | 1:100 | Corns, L.F., et al. 2015. Cholinergic Enhancement of Cell Proliferation in the Postnatal Neurogenic Niche of the Mammalian Spinal Cord.. Stem Cells., 2864-76. |
| 24445073 | 1:500 | Robles, C.F., et al. 2014. Effects of kappa opioid receptors on conditioned place aversion and social interaction in males and females.. Behav Brain Res., 84-93. |
| 24336719 | 1:2000 | Gunn, B.G., et al. 2013. Dysfunctional astrocytic and synaptic regulation of hypothalamic glutamatergic transmission in a mouse model of early-life adversity: relevance to neurosteroids and programming of the stress response.. J Neurosci., 19534-54. |
| 24259590 | 1:1000 | Hagemann, T.L., et al. 2013. Deficits in adult neurogenesis, contextual fear conditioning, and spatial learning in a Gfap mutant mouse model of Alexander disease.. J Neurosci., 18698-706. |
| 23840874 | 1:500 | Cho, S.I., et al. 2013. Mechanisms of hearing loss after blast injury to the ear. Plos One, e67618. |
| 23542689 | 1:500 | Kang, S.H., et al. 2013. Degeneration and impaired regeneration of gray matter oligodendrocytes in amyotrophic lateral sclerosis.. Nat Neurosci., 571–579. |
| 23459611 | 1:1000 | Huang, L., et al. 2013. Reciprocal connectivity between mitral cells and external plexiform layer interneurons in the mouse olfactory bulb.. Front Neural Circuits., . |
| 23459611 | 1:1000 | Huang, L., et al. 2013. Reciprocal connectivity between mitral cells and external plexiform layer interneurons in the mouse olfactory bulb.. Frontiers in neural circuits, 32. |
| 23122958 | 1:200 | Chen, R., et al. 2012. Monoacylglycerol lipase is a therapeutic target for Alzheimer''s disease.. Cell Rep., 1329-39. |
| 23100420 | 1:400 | Ma, S., et al. 2012. Ric-8a, a guanine nucleotide exchange factor for heterotrimeric G proteins, regulates bergmann glia-basement membrane adhesion during cerebellar foliation.. J Neurosci., 14979-93. |
| 23039175 | 1:200 | Lu, J., et al. 2012. Pain in experimental autoimmune encephalitis: a comparative study between different mouse models.. J Neuroinflammation., . |
| 21436042 | 1:1000 | Ramsey, A.J., et al. 2011. Impaired NMDA receptor transmission alters striatal synapses and DISC1 protein in an age-dependent manner.. Proc Natl Acad Sci U S A., 5795-800. |
| Immunohistochemistry: Non-Human Primate | ||
| PMID | Dilution | Publication |
| 31589838 | 1:100 | Peterson, S.M., et al. 2019. Bardet-Biedl Syndrome in rhesus macaques: A nonhuman primate model of retinitis pigmentosa. Experimental Eye Research, 107825. |
| Immunohistochemistry: Polar Bear | ||
| PMID | Dilution | Publication |
| 26313569 | 1:2000 | Prüss, H., et al. 2015. Anti-NMDA receptor encephalitis in the polar bear (Ursus maritimus) Knut. . Scientific reports, 12805. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 35105675 | 1:500 | Wang, L., et al. 2022. Anastasis drives senescence and non-cell autonomous neurodegeneration in the astrogliopathy Alexander disease.. Journal of Neuroscience, 2584-2597. |
| 32730865 | 1:1000 | Stanisavljević, A., et al. 2020. Fluoxetine modulates neuronal activity in stress-related limbic areas of adult rats subjected to the chronic social isolation. Brain Research Bulliten, 95-108. |
| 31682945 | 1:300 | Dragić, M., et al. 2019. Two Distinct Hippocampal Astrocyte Morphotypes Reveal Subfield-Different Fate during Neurodegeneration Induced by Trimethyltin Intoxication. Neurosciecne, 38-54. |
| 31624998 | 1:500 | Bozic, I., et al. 2019. The Potassium Channel Kv1.5 Expression Alters During Experimental Autoimmune Encephalomyelitis. Neurochemical Research, 2733-2745. |
| 30460639 | 1:400 | Dakic, T., et al. 2019. Food For Thought: Short-Term Fasting Upregulates Glucose Transporters in Neurons and Endothelial Cells, But Not in Astrocytes. Neurochemical Research, 388-399. |
| 29574670 | 1:500 | Bozic, I., et al. 2018. Voltage Gated Potassium Channel Kv1.3 Is Upregulated on Activated Astrocytes in Experimental Autoimmune Encephalomyelitis. Neurochemical Research, 1020-1034. |
| 29567092 | 1:250 | Logan, C.N., et al. 2018. Nucleus accumbens GLT-1a overexpression reduces glutamate efflux during reinstatement of cocaine-seeking but is not sufficient to attenuate reinstatement. Neuropharmacology, 297-307. |
| 29345029 | 1:1000 | Lütt, A., et al. 2018. High prevalence and functional effects of serum antineuronal antibodies in patients with gastrointestinal disorders. Gastroenterology and motility, e13292. |
| 29163045 | 1:500 | Jakovljevic, M., et al. 2017. Down-regulation of NTPDase2 and ADP-sensitive P2 Purinoceptors Correlate with Severity of Symptoms during Experimental Autoimmune Encephalomyelitis. Frontiers in Cellular Neuroscience, . |
| 28965011 | 1:500 | Djedović, N., et al. 2017. Anti-encephalitogenic effects of ethyl pyruvate are reflected in the central nervous system and the gut. Biomedicine & Pharmacotherapy, 78-85. |
| 28578430 | 1:500 | Lavrnja, I., et al. 2017. Expression profiles of cholesterol metabolism-related genes are altered during development of experimental autoimmune encephalomyelitis in the rat spinal cord. . Scientific reports, 2702. |
| 28544147 | 1:500 | Dakic, T.B., et al. 2017. Short-term fasting promotes insulin expression in rat hypothalamus. European Journal of Neuroscience, 1730-1737. |
| 28544147 | 1:500 | Dakic, T.B., et al. 2017. Short-term fasting promotes insulin expression in rat hypothalamus. European Journal of Neuroscience, 1730-1737. |
| 24860436 | 1:500 | Seifi, M., et al. 2014. Localization of NG2 immunoreactive neuroglia cells in the rat locus coeruleus and their plasticity in response to stress.. Frontiers in Neuroanatomy, . |
| 23025758 | not listed | Baalman, K.L., et al. 2013. Blast wave exposure impairs memory and decreases axon initial segment length.. Journal of Neurotrauma, 741-751. |
| 22675541 | not listed | Manning, C.F., et al. 2012. Benefits and pitfalls of secondary antibodies: why choosing the right secondary is of primary importance.. PLoS One., e38313. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 35511821 | not listed | Yang, A.W., et al. 2022. Effects of Alexander disease-associated mutations on the assembly and organization of GFAP intermediate filaments. Molecular biology of the cell, mbcE22010013. |
| 29634780 | 1:1000 | Keren-Aviram, G., et al. 2018. Proteomic analysis of human epileptic neocortex predicts vascular and glial changes in epileptic regions. PLOS One, e0195639. |
| 28111074 | not listed | Huang, Y.W.A., et al. 2017. ApoE2, ApoE3, and ApoE4 differentially stimulate APP transcription and Aβ secretion. . Cell, 427-441. |
| 25864570 | 1:2000 | Ristić, A.J., et al. 2015. Hippocampal antioxidative system in mesial temporal lobe epilepsy.. Epilepsia., 789-99. |
| 29634780 | 1:1000 | Keren-Aviram, G., et al. 2018. Proteomic analysis of human epileptic neocortex predicts vascular and glial changes in epileptic regions.. Plos one, e0195639. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 35511821 | not listed | Yang, A.W., et al. 2022. Effects of Alexander disease-associated mutations on the assembly and organization of GFAP intermediate filaments. Molecular biology of the cell, mbcE22010013. |
| 32447612 | 1:10,000 | Gruol, D.L., et al. 2020. Alcohol alters IL-6 Signal Transduction in the CNS of Transgenic Mice with Increased Astrocyte Expression of IL-6. Cellular and Molecular Neuobiology, 733-750. |
| 31437534 | 1:10,000 | Roberts, A.J., et al. 2019. Increased IL-6 expression in astrocytes is associated with emotionality, alterations in central amygdala GABAergic transmission, and excitability during alcohol withdrawal. . Brain, Behavior, and Immunity., 188-202. |
| 29787738 | 1:10,000 | Gruol, D.L., et al. 2018. Altered Brain Activity During Withdrawal from Chronic Alcohol is Associated with Changes in IL-6 Signal Transduction and GABAergic Mechanisms in Transgenic Mice with Increased Astrocyte Expression of IL-6. Neuropharmacology., 32-46. |
| 28111074 | not listed | Huang, Y.W.A., et al. 2017. ApoE2, ApoE3, and ApoE4 Differentially Stimulate APP Transcription and Aβ Secretion. Cell, 427-441. |
| 24259518 | 1:2000 | Dagley, L.F., et al. 2014. Quantitative proteomic profiling reveals novel region-specific markers in the adult mouse brain.. Proteomics., 241-261. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 34485605 | 1:1000 | Hooper, A.W., et al. 2021. Gene therapy using an ortholog of human fragile X mental retardation protein partially rescues behavioral abnormalities and EEG activity.. Molecular Therapy-Methods & Clinical Development, 196-209. |
| 26241743 | 1:6000 | Galbavy, W., et al. 2015. Neuroimmune and Neuropathic Responses of Spinal Cord and Dorsal Root Ganglia in Middle Age.. PLoS One., e0134394. |
| 24639151 | 1:500 | Hamity, M.V., et al. 2014. Increased neuronal expression of neurokinin-1 receptor and stimulus-evoked internalization of the receptor in the rostral ventromedial medulla of the rat after peripheral inflammatory injury.. J Comp Neurol., 3037-51. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 25393884 | Savic, D., et al. 2014. Ribavirin shows immunomodulatory effects on activated microglia.. Immunopharmacol Immunotoxicol., 433-41. | |
| 33908669 | Lin, N.H., et al. 2021. Elevated GFAP isoform expression promotes protein aggregation and compromises astrocyte function.. The FASEB Journal, e21614. | |