Anti-GFAP Antibody (N206A/8)
Primary Antibody
Human, Mouse, Rat
N206A/8
ICC, IHC, WB
Mouse
Purified
GFAP
50 kDa
Adult WT and KO mouse hippocampus immunofluorescence with N206A/8 (green) and KC rabbit anti- Kv2.1 (red). Brain samples courtesy of Albee Messing, University of Wisconsin at Madison.
Product Specific References for Applications and Species
- FACS: Human
- Immunocytochemistry: Human | Mouse | Rat
- Immunohistochemistry: Human | Mouse | Non-Human Primate | Rat
- Western Blot: Mouse
- Additional Publications: Unspecified
| FACS: Human | ||
| PMID | Dilution | Publication |
| 28757165 | 1:2000 | TCW, J., et al. 2017. An Efficient Platform for Astrocyte Differentiation from Human Induced Pluripotent Stem Cells. Stem Cell Reports, 600-614. |
| 28757163 | 1:1000 | Ho, S.M., et al. 2017. Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes. Stem Cell Reports, 615-628. |
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 37527657 | 1:1000 | Byun, YG, et al. 2023. Stress induces behavioral abnormalities by increasing expression of phagocytic receptor, MERTK, in astrocytes to promote synapse phagocytosis. Immunity, 0. |
| 37300770 | 1:5000 | Vulakh, G, et al. 2023. Characterizing the Neuron-Glial Interactions by the Co-cultures of Human iPSC-Derived Oligodendroglia and Neurons. Methods in Molecular Biology, 103-111. |
| 36736291 | 1:1000 | Linares, G.R., et al. 2023. SYF2 Suppression Mitigates Neurodegeneration in Models of Diverse Forms of ALS. Cell Stem Cell, 171-187. |
| 30355486 | 1:250 | Mehta, S.R., et al. 2018. Human Huntington''''s Disease iPSC-Derived Cortical Neurons Display Altered Transcriptomics, Morphology, and Maturation. Cell Reports, 1081-1096. |
| 29706869 | not listed | Yu, Q., et al. 2018. Fibronectin Promotes the Malignancy of Glioma Stem-Like Cells Via Modulation of Cell Adhesion, Differentiation, Proliferation and Chemoresistance. Frontiers in Molecular Neuroscience, 130. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 36707536 | 1:250 | Shahin, W.S., et al. 2023. Redox-dependent Igfbp2 signaling controls Brca1 DNA damage response to govern neural stem cell fate. Nature Communications, 444. |
| 35952494 | not listed | Silva Oliveira Junior, M., et al. 2022. Myelin repair is fostered by the corticosteroid medrysone specifically acting on astroglial subpopulations. EBioMedicine, 104204. |
| 31500698 | not listed | Yu, H., et al. 2019. MRGPRX4 is a bile acid receptor for human cholestatic itch.. Elife, e48431. |
| 31433979 | not listed | Abdi, K., et al. 2019. EGFR Signaling Termination via Numb Trafficking in Ependymal Progenitors Controls Postnatal Neurogenic Niche Differentiation.. Cell Reports, 2012-2022. |
| 30314515 | 1:500 | Putatunda, R., et al. 2018. Adult neurogenic deficits in HIV-1 Tg26 transgenic mice. Journal of Neuroinflammation, 287. |
| 26999761 | 1:1000 | Lassiter, C.M., et al. 2016. Embryonic stem cell-derived neural progenitors transplanted to the hippocampus migrate on host vasculature. Stem Cell Research, 579-88. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 29251594 | 1:2000 | Lin, J.P., et al. 2017. LRP1 regulates peroxisome biogenesis and cholesterol homeostasis in oligodendrocytes and is required for proper CNS myelin development and repair. eLife, e30498. |
| Immunohistochemistry: Human | ||
| PMID | Dilution | Publication |
| 36303069 | 1:200 | Leng, K., et al. 2022. CRISPRi Screens in Human iPSC-derived Astrocytes Elucidate Regulators of Distinct Inflammatory Reactive States. Nature Neuroscience, 1528-1542. |
| 33239403 | 1:500 | Fracassi, A., et al. 2021. Oxidative Damage and Antioxidant Response in Frontal Cortex of Demented and Nondemented Individuals with Alzheimer's Neuropathology. The Journal of Neuroscience, 538-554. |
| 28216146 | 1:1000 | TCW, J., et al. 2017. Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis. Stem Cell Reports, 519-528. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 37527657 | 1:1000 | Byun, YG, et al. 2023. Stress induces behavioral abnormalities by increasing expression of phagocytic receptor, MERTK, in astrocytes to promote synapse phagocytosis. Immunity, 0. |
| 37422764 | 1:500 | Naffaa, MM, et al. 2023. Cortical regulation of neurogenesis and cell proliferation in the ventral subventricular zone. Cell reports, 112783. |
| 37043357 | 1:400 | Genet, N., et al. 2023. Connexin 43-Mediated Neurovascular Interactions Regulate Neurogenesis in the Adult Brain Subventricular Zone. Cell Reports, 112371. |
| 37037362 | 1:1000 | Abe, C., et al. 2023. Repeated Activation of C1 Neurons in Medulla Oblongata Decreases Anti-Inflammatory Effect Via the Hypofunction of the Adrenal Gland Adrenergic Response. Brain, Behavior, and Immunity, 138-150. |
| 36941428 | 1:500 | Chen, Z.P., et al. 2023. Lipid-Accumulated Reactive Astrocytes Promote Disease Progression in Epilepsy. Nature Neuroscience, 542-554. |
| 36762625 | 1:1000 | Gnanaguru, G., et al. 2023. Microglia Refine Developing Retinal Astrocytic and Vascular Networks Through the Complement C3/C3aR Axis. Development, dev201047. |
| 36405424 | 1:10000 | Carpenter, J.M., et al. 2022. Evaluation of Delayed LNFPIII Treatment Initiation Protocol on Improving Long-Term Behavioral and Neuroinflammatory Pathology in a Mouse Model of Gulf War Illness. Brain, Behavior, & Immunity, 100553. |
| 36370710 | 1:500 | Wang, X., et al. 2022. Driving Axon Regeneration By Orchestrating Neuronal And Non-Neuronal Innate Immune Responses Via The IFNγ-cGAS-STING Axis. Neuron, . |
| 36303069 | 1:100 | Leng, K., et al. 2022. CRISPRi Screens in Human iPSC-derived Astrocytes Elucidate Regulators of Distinct Inflammatory Reactive States. Nature Neuroscience, 1528-1542. |
| 36287323 | 1:1000 | Qu, W., et al. 2022. Deletion of Small GTPase H-Ras Rescues Memory Deficits and Reduces Amyloid Plaque-Associated Dendritic Spine Loss in Transgenic Alzheimer's Mice. Molecular Neurobiology, . |
| 36258255 | not listed | Ferguson, S., et al. 2022. Impact of Gulf War Toxic Exposures After Mild Traumatic Brain Injury. Acta Neuropathologica Communications, 147. |
| 36210210 | 1:1000 | Yamamoto-Fukuda, T., et al. 2022. Keratinocyte Growth Factor Stimulates Growth of p75+ Neural Crest Lineage Cells During Middle Ear Cholesteatoma Formation in Mice. The American Journal of Pathology, . |
| 34471138 | 1:500 | Ronzano, R., et al. 2021. Microglia-neuron interaction at nodes of Ranvier depends on neuronal activity through potassium release and contributes to remyelination. Nature Communications, 5219. |
| 34387945 | 1:2000 | Reitz, S.J., et al. 2021. Enhanced Multiplexing of Immunofluorescence Microscopy Using a Long-Stokes-Shift Fluorophore. Current Protocols, e214. |
| 33728392 | 1:600 | Ehsanipour, A., et al. 2021. Injectable, macroporous scaffolds for delivery of therapeutic genes to the injured spinal cord. APL Bioengineering, 16104. |
| 30999016 | 1:500 | Putatunda, R., et al. 2019. Sex-specific neurogenic deficits and neurocognitive disorders in middle-aged HIV-1 Tg26 transgenic mice.. Brain, Behavior, and Immunity, 488-499. |
| 30472209 | 1:1000 | Yu, F.P.S., et al. 2019. Acid Ceramidase Deficiency in Mice Leads to Severe Ocular Pathology and Visual Impairment. The American Journal of Pathology, 320-338. |
| 30610918 | 1:1000 | Dumont, C.M., et al. 2018. Aligned hydrogel tubes guide regeneration following spinal cord injury. Acta Biomaterialia, 312-322. |
| 30465881 | 1:500 | Hill, J.D., et al. 2018. Activation of GPR55 induces neuroprotection of hippocampal neurogenesis and immune responses of neural stem cells following chronic, systemic inflammation. Brain, Behavior, and Immunity, 165-181. |
| 30453022 | 1:500 | Zhang, B., et al. 2018. Reducing age-dependent monocyte-derived macrophage activation contributes to the therapeutic efficacy of NADPH oxidase inhibition in spinal cord injury. Brain, Behavior, and Immunity, 139-150. |
| 30364309 | 1:10000 | Morin, A., et al. 2018. Treatment With Nilvadipine Mitigates Inflammatory Pathology and Improves Spatial Memory in Aged hTau Mice After Repetitive Mild TBI. Frontiers in Aging Neuroscience, 292. |
| 30314515 | 1:500 | Putatunda, R., et al. 2018. Adult neurogenic deficits in HIV-1 Tg26 transgenic mice. Journal of Neuroinflammation, 287. |
| 29505961 | 1:500 | Patel, N.S., et al. 2018. Identification of new phosphodiesterase 9A (PDE9A) isoforms and how their expression and subcellular compartmentalization in brain changes across the lifespan. Neurobiology of Aging, 217-234. |
| 29463313 | 1:500 | Xiao, X., et al. 2018. Lymphotoxin β receptor-mediated NFκB signaling promotes glial lineage differentiation and inhibits neuronal lineage differentiation in mouse brain neural stem/progenitor cells. Journal of Neuroinflammation, 49. |
| 29379059 | not listed | Yu, F.P.S., et al. 2018. Deletion of MCP-1 Impedes Pathogenesis of Acid Ceramidase Deficiency. Scientific Reports, 1808. |
| 29089377 | 1:1000 | Nakamuta, S., et al. 2017. Dual role for DOCK7 in tangential migration of interneuron precursors in the postnatal forebrain. Journal of Cell Biology, 4313-4330. |
| 28877763 | 1:5000 | Schweig, J.E., et al. 2017. Alzheimer's disease pathological lesions activate the spleen tyrosine kinase. Acta Neuropathologica Communications, 69. |
| 28809400 | not listed | Alachkar, A., et al. 2017. Prenatal one-carbon metabolism dysregulation programs schizophrenia-like deficits. Molecular Psychiatry, 282-294. |
| 28456715 | 1:500 | Szot, P., et al. 2017. Multiple lipopolysaccharide (LPS) injections alters interleukin 6 (IL-6), IL-7, IL-10 and IL-6 and IL-7 receptor mRNA in CNS and spleen. Neuroscience, 45190. |
| 28402856 | 1:1000 | Platt, R.J., et al. 2017. Chd8 Mutation Leads to Autistic-like Behaviors and Impaired Striatal Circuits. Cell Reports, 335-350. |
| 28223982 | 1:2000 | Ronca, S.E., et al. 2017. Mouse Model of Neurological Complications Resulting from Encephalitic Alphavirus Infection. Frontiers in Microbiology, 188. |
| 27339393 | not listed | Hegde, S., et al. 2016. Phosphodiesterase 11A (PDE11A), Enriched in Ventral Hippocampus Neurons, is Required for Consolidation of Social but not Nonsocial Memories in Mice. Neuropsychopharmacology, 2920-2931. |
| 25451251 | 1:1000 | Yamada, H., et al. 2014. Transplantation of mature adipocyte-derived dedifferentiated fat cells promotes locomotor functional recovery by remyelination and glial scar reduction after spinal cord injury in mice. Biochemical and Biophysical Research Communications, 341-346. |
| Immunohistochemistry: Non-Human Primate | ||
| PMID | Dilution | Publication |
| 29535375 | 1:500 | Nagahara, A.H., et al. 2017. MR-guided delivery of AAV2-BDNF into the entorhinal cortex of non- human primates. Gene Therapy, 104-114. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 36521513 | 1:3000 | Humphrey, C.M., et al. 2022. Synaptic Loss and Gliosis in the Nucleus Tractus Solitarii with Streptozotocin-induced Alzheimer's Disease. Brain Research, 148202. |
| 34571884 | not listed | Torrillas de la Cal, A., et al. 2021. The Structure of the Spinal Cord Ependymal Region in Adult Humans Is a Distinctive Trait among Mammals. Cells, 2235. |
| 30611291 | 1:500 | Peruzzaro, S.T., et al. 2019. Transplantation of mesenchymal stem cells genetically engineered to overexpress interleukin-10 promotes alternative inflammatory response in rat model of traumatic brain injury. Journal of Neuroinflammation, 2. |
| 30489213 | 1:500 | Walker, C.L., et al. 2019. Functional and Histological Gender Comparison of Age-Matched Rats after Moderate Thoracic Contusive Spinal Cord Injury. Journal of Neurotrauma, 1974-1984. |
| 29485103 | 1:250 | Lo, M.C., et al. 2018. Evaluating the in vivo glial response to miniaturized parylene cortical probes coated with an ultra-fast degrading polymer to aid insertion. Journal of Neural Engineering, 36002. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 36287323 | 1:500 | Qu, W., et al. 2022. Deletion of Small GTPase H-Ras Rescues Memory Deficits and Reduces Amyloid Plaque-Associated Dendritic Spine Loss in Transgenic Alzheimer's Mice. Molecular Neurobiology, . |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 35568187 | Madalena, K.M., et al. 2022. Genetic deletion of the glucocorticoid receptor in Cx3cr1+ myeloid cells is neuroprotective and improves motor recovery after spinal cord injury. Experimental neurology, 114114. | |
| 35275429 | Murlanova, K., et al. 2022. Deficient mitochondrial respiration in astrocytes impairs trace fear conditioning and increases naloxone-precipitated aversion in morphine-dependent mice. Glia, 1289-1300. | |
| 30633922 | Bao, Y., et al. 2019. Methyl 3,4-dihydroxybenzoate protects retina in a mouse model of acute ocular hypertension through multiple pathways. Experimental Eye Research, 15-24. | |
| 29913164 | Zhang, Y., et al. 2018. Structural and visual functional deficits in a rat model of neuromyelitis optica spectrum disorders related optic neuritis. Experimental Eye Research, 124-132. | |
| 24639124 | Hasegawa, Y., et al. 2014. Spatiotemporal Distribution of SUMOylation Components during Mouse Brain Development. The Journal of Comparative Neurology Research in Systems Neuroscience, 3020-3036. | |