Name: Anti-Gap-43 (Ser41) Antibody
Brand: PhosphoSolutions
SKU: p1150-41
Price: 415.0 USD
Availability: InStock

Our Anti-Gap-43 (Ser41) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Gap-43 (Ser41) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Xenopus, Zebrafish

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

GAP43

Molecular Weight

50 kDa

Go to Full Product Details

Western blot of rat cortical lysate showing specific immunolabeling of the ~50 kDa Gap-43 protein phosphorylated at Ser41 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Gap-43 (Ser41) Antibody

Product Details

Target

Gap-43 (Ser41)

Target Description

Gap-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that Gap-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). Gap-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser-41 by protein kinase C (Catalog No. 1609-PKC) modulates the interaction of Gap-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

GAP43

Molecular Weight

50 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser41of rat GAP43, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Xenopus, Zebrafish

Antibody Registry ID

AB_2492123

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~50 kDa Gap-43 protein phosphorylated at Ser41. In some tissues the antibody also recognizes a higher molecular weight protein that is also recognized by the pan Gap-43 antibody, which may be a Gap-43 aggregate or oligomer. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser41

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Axonal membrane protein GAP 43 antibody, Axonal membrane protein GAP-43 antibody, B 50 antibody, Calmodulin binding protein P 57 antibody, F1 antibody, GAP 43 antibody, GAP-43 antibody, GAP43 antibody, Growth Associated Protein 43 antibody, Growth-associated protein 43 antibody, Nerve Growth Related Peptide antibody, Nerve growth related peptide GAP43 antibody, NEUM_HUMAN antibody, Neural phosphoprotein B 50 antibody, Neural phosphoprotein B-50 antibody, Neuromodulin antibody, Neuron growth associated protein 43 antibody, PP46 antibody, Protein F1 antibody, QtrA-11580 antibody, QtrA-13071 antibody

UniProt Details

UniProt (Human): Q5U058
UniProt (Immunogen Species): P07936

NCBI Gene ID

29423

Product Specific References for Applications and Species

Western Blot: Rat

Western Blot: Rat
PMID	Dilution	Publication
18375950	1:1000	Gopalakrishna, R., et al. 2008. A direct redox regulation of protein kinase C isoenzymes mediates oxidant-induced neuritogenesis in PC12 cells. Journal of Biological Chemistry, 283(21), pp.14430-14444.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Gap-43 (Ser41) Antibody

Our Anti-Gap-43 (Ser41) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Gap-43 (Ser41) and is antigen affinity purified from pooled serum. It is great for use in WB.