Name: Anti-GABAB Receptor (Ser783), R2-Subunit Antibody
Brand: PhosphoSolutions
SKU: p1148-783
Price: 415.0 USD
Availability: InStock

Our Anti-GABAB Receptor (Ser783), R2-Subunit rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat GABAB Receptor (Ser783), R2-Subunit and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Xenopus

Applications

ICC, IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

GABBR2

Molecular Weight

102 kDa

Go to Full Product Details

Western blot of rat synaptic membrane lysate showing specific immunolabeling of the ~102 kDa GABAB R2 protein phosphorylated at Ser783 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-GABAB Receptor (Ser783), R2-Subunit Antibody

Product Details

Target

GABAB Receptor (Ser783), R2-Subunit

Target Description

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABA-A and the GABA-B subtype of receptors. GABA-B receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. It has recently been demonstrated that AMPK binds directly to GABA-B receptors and phosphorylates Ser-783 in the cytoplasmic tail of the R2 subunit and that Ser-783 plays a critical role in enhancing neuronal survival after ischemia as phosphorylation of Ser-783 is evident in many brain regions and is increased dramatically after ischemic injury to the brain (Kuramoto et al., 2007).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

GABBR2

Molecular Weight

102 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser783 of the R2 subunit of rat GABAB recerptor, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Xenopus

Antibody Registry ID

AB_2492117

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~102 kDa GABAB R2 phosphorylated at Ser783. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser783

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

BcDNA:GH07312 antibody, CG6706 antibody, CT20836 antibody, D Gaba2 antibody, FLJ36928 antibody, G protein coupled receptor 51 antibody, G-protein coupled receptor 51 antibody, GAB B R2 antibody, GABA B R2 antibody, GABA B receptor 2 antibody, GABA-B receptor 2 antibody, GABA-B-R2 antibody, GABA-BR2 antibody, GABAB R2 antibody, GABABR 2 antibody, GABABR2 antibody, GABB R2 antibody, GABBR 2 antibody, Gabbr2 antibody, GABR2_HUMAN antibody, Gamma aminobutyric acid B receptor 2 antibody, Gamma aminobutyric acid GABA B receptor 2 antibody, Gamma aminobutyric acid type B receptor subunit 2 antibody, Gamma-aminobutyric acid type B receptor subunit 2 antibody, Gb 2 antibody, Gb2 antibody, GH07312 antibody, GPR 51 antibody, GPR51 antibody, GPRC 3B antibody, GPRC3B antibody, HG 20 antibody, HG20 antibody, HRIHFB2099 antibody, Metabotropic GABA B receptor subtype 2 antibody, OTTHUMP00000021776 antibody, OTTHUMP00000063797 antibody, R2 SUBUNIT antibody

UniProt Details

UniProt (Human): O75899
UniProt (Immunogen Species): O88871

NCBI Gene ID

83633

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Immunohistochemistry: Rat
Western Blot: Mouse | Rat

Immunocytochemistry: Rat
PMID	Dilution	Publication
17224405	1:500	Kuramoto, N., et al. 2007. Phospho-dependent functional modulation of GABA(B) receptors by the metabolic sensor AMP-dependent protein kinase. Neuron, 53:233-247.

Immunohistochemistry: Rat
PMID	Dilution	Publication
17224405	1:500	Kuramoto, N., et al. 2007. Phospho-dependent functional modulation of GABA(B) receptors by the metabolic sensor AMP-dependent protein kinase. Neuron, 53:233-247.

Western Blot: Mouse
PMID	Dilution	Publication
25843643	1:200	Marron Fernandez de Velasco, E., et al. 2015. Sex differences in GABA B R-GIRK signaling in layer 5/6 pyramidal neurons of the mouse prelimbic cortex. Neuropharmacology. Aug;95:353-60.
25261019	1:500	Terunuma, M., et al. 2015. Purinergic receptor activation facilitates astrocytic GABA_B receptor calcium signaling. Neuropharmacology 88: 74-81.
24431439	1:500	Terunuma, M., et al. 2014. Postsynaptic GABAB receptor activity regulates excitatory neuronal architecture and spatial memory. J Neurosci. 34: 804-816.
22405207	1:500	Padgett, C.L., et al. 2012. Postsynaptic GABAB receptor activity regulates excitatory neuronal architecture and spatial memory. J Neurosci. 34: 804-816.

Western Blot: Rat
PMID	Dilution	Publication
31678999	1:1000	Kim, S.H., et al. 2020. Electroconvulsive seizures induce autophagy by activating the AMPK signaling pathway in the rat frontal cortex. International Journal of Neuropsychopharmacology, 23(1), pp.42-52.
29079139	1:1000	Kim, S.H., et al. 2018. The antipsychotic agent clozapine induces autophagy via the AMPK-ULK1-Beclin1 signaling pathway in the rat frontal cortex. Progress in Neuro-Psychopharmacology and Biological Psychiatry. Feb 2;81:96-104.
20643948	1:500	Terunuma, M., et al. 2010. Prolonged activation of NMDA receptors promotes dephosphorylation and alters postendocytic sorting of GABAB receptors. Proc Natl Acad Sci USA 107: 13918-13923.
17224405	1:500	Kuramoto, N., et al. 2007. Phospho-dependent functional modulation of GABA(B) receptors by the metabolic sensor AMP-dependent protein kinase. Neuron, 53:233-247.

Product Specific Protocols

Western Blot Protocol: Download

Recover password

Create my account

Anti-GABAB Receptor (Ser783), R2-Subunit Antibody

Our Anti-GABAB Receptor (Ser783), R2-Subunit rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat GABAB Receptor (Ser783), R2-Subunit and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.