Name: Anti-GABAA Receptor γ2 (Ser327) Antibody
Brand: PhosphoSolutions
SKU: p1130-327
Price: 415.0 USD
Availability: InStock

Our Anti-GABAA Receptor γ2 (Ser327) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat GABAA Receptor γ2 (Ser327) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

GABRG2

Molecular Weight

45 kDa

Go to Full Product Details

Western blot of rat cortical lysate showing specific immunolabeling of the ~45 kDa GABAA γ2 protein phosphorylated at Ser327 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-GABAA Receptor γ2 (Ser327) Antibody

Product Details

Target

GABAA Receptor γ2 (Ser327)

Target Description

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABA-A and the GABA-AB subtype of receptors. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. It has recently been suggested that PKCε regulates the sensitivity of GABA-A α1β2γ2 receptors to ethanol and benzodiazepines through phosphorylation of serine 327 in the large intracellular loop of γ2 (Qi et al., 2007)

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

GABRG2

Molecular Weight

45 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327 of the γ2 subunit of rat GABAA receptor, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate

Antibody Registry ID

AB_2492116

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~45 kDa GABAA receptor γ2 subunit phosphorylated at Ser327. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser327

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

CAE 2 antibody, CAE2 antibody, ECA 2 antibody, ECA2 antibody, GABA(A) receptor gamma 2 antibody, GABA(A) receptor subunit gamma 2 antibody, GABA(A) receptor subunit gamma-2 antibody, GABRγ2 antibody, GABRG2 antibody, Gamma aminobutyric acid (GABA) A receptor gamma 2 antibody, Gamma aminobutyric acid A receptor gamma 2 antibody, Gamma aminobutyric acid receptor gamma 2 subunit antibody, Gamma-aminobutyric acid receptor subunit gamma-2 antibody, GBRG2_HUMAN antibody, GEFSP 3 antibody, GEFSP3 antibody

UniProt Details

UniProt (Human): P18507
UniProt (Immunogen Species): P18508

NCBI Gene ID

29709

Product Specific References for Applications and Species

Western Blot: Mouse | Rat

Western Blot: Mouse
PMID	Dilution	Publication
17875639	1:1000	Qi, Z.H., et al. 2007. Protein kinase Cε regulates γ-aminobutyrate type A receptor sensitivity to ethanol and benzodiazepines through phosphorylation of γ2 subunits. J Biol Chem, 282(45):33052-63.

Western Blot: Rat
PMID	Dilution	Publication
36787750	1:500	Rosenberg, E.C., et al. 2023. Cannabidiol Modulates Excitatory-Inhibitory Ratio to Counter Hippocampal Hyperactivity. Neuron, .
26767953	1:1000	Bohnsack, J.P., et al. 2016. Differential regulation of synaptic and extrasynaptic α4 GABA (A) receptor populations by protein kinase A and protein kinase C in cultured cortical neurons. Neuropharmacology, 105, 124-132.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-GABAA Receptor γ2 (Ser327) Antibody

Our Anti-GABAA Receptor γ2 (Ser327) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat GABAA Receptor γ2 (Ser327) and is antigen affinity purified from pooled serum. It is great for use in WB.