Our Anti-GABAA Receptor γ2 (Ser327) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat GABAA Receptor γ2 (Ser327) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat cortical lysate showing specific immunolabeling of the ~45 kDa GABAA γ2 protein phosphorylated at Ser327 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABA-A and the GABA-AB subtype of receptors. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. It has recently been suggested that PKCε regulates the sensitivity of GABA-A α1β2γ2 receptors to ethanol and benzodiazepines through phosphorylation of serine 327 in the large intracellular loop of γ2 (Qi et al., 2007)
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
GABRG2
45 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327 of the γ2 subunit of rat GABAA receptor, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Bovine, Canine, Chicken, Human, Non-Human Primate
AB_2492116
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~45 kDa GABAA receptor γ2 subunit phosphorylated at Ser327. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Phosphorylated
Ser327
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Qi, Z.H., et al. 2007. Protein kinase Cε regulates γ-aminobutyrate type A receptor sensitivity to ethanol and benzodiazepines through phosphorylation of γ2 subunits. J Biol Chem, 282(45):33052-63.
Bohnsack, J.P., et al. 2016. Differential regulation of synaptic and extrasynaptic α4 GABA (A) receptor populations by protein kinase A and protein kinase C in cultured cortical neurons. Neuropharmacology, 105, 124-132.
