Anti-GABAA Receptor α1, N-Terminus Antibody

Name: Anti-GABAA Receptor α1, N-Terminus Antibody
Brand: PhosphoSolutions
SKU: 812-GA1N
Price: 415.0 USD
Availability: InStock

from PhosphoSolutions Datasheet KO Validated Trial Size

6 Citations

Our Anti-GABAA Receptor α1, N-Terminus primary antibody from PhosphoSolutions is rabbit polyclonal. It detects mouse and rat GABAA Receptor α1, N-Terminus and is antigen affinity purified. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Human, Non-Human Primate

Applications

IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

GABRA1

Molecular Weight

51 kDa

Go to Full Product Details

Western blot of mouse forebrain lysates from Wild Type (WT) and α1-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa α1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α1-knockout animals.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-GABAA Receptor α1, N-Terminus Antibody

Product Details

Target

GABAA Receptor α1, N-Terminus

Target Description

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

GABRA1

Molecular Weight

51 kDa

Antigen

Synthetic peptide corresponding to amino acid residues from the N-terminal region of the α1 subunit of rat GABAA, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Human, Non-Human Primate

Antibody Registry ID

AB_2492100

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:100

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~51 kDa α1-subunit of the GABAA receptor. Immunolabeling is absent in α1-subunit knockout animals.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

ECA4 antibody, EIEE19 antibody, EJM antibody, EJM5 antibody, Gaba receptor alpha 1 polypeptide antibody, GABA(A) receptor antibody, GABA(A) receptor subunit alpha 1 antibody, GABA(A) receptor subunit alpha-1 antibody, GABA(A) receptor, alpha 1 antibody, GABRA 1 antibody, GABRα1 antibody, Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody, Gamma aminobutyric acid A receptor alpha 1 antibody, Gamma aminobutyric acid type A receptor alphα1 subunit antibody

UniProt Details

UniProt (Human): P14867
UniProt (Immunogen Species): P62813

NCBI Gene ID

29705

Product Specific References for Applications and Species

Immunohistochemistry: Mouse | Rat
Western Blotting: Mouse | Rat

Immunohistochemistry: Mouse
PMID	Dilution	Publication
32041742	1:100	Agoglia, A.E., et al. 2020. Corticotropin-Releasing Factor Receptor-1 Neurons in the Lateral Amygdala Display Selective Sensitivity to Acute and Chronic Ethanol Exposure. Eneuro, 7(2).

Immunohistochemistry: Rat
PMID	Dilution	Publication
25170988	1:100	Herman, M., et al. 2016. Cell‐type‐specific tonic GABA signaling in the rat central amygdala is selectively altered by acute and chronic ethanol. Addiction biology. Jan;21(1):72-86.

Western Blotting: Mouse
PMID	Dilution	Publication
26446222	not listed	Engin, E., et al. 2015. Tonic Inhibitory Control of Dentate Gyrus Granule Cells by α5-Containing GABAA Receptors Reduces Memory Interference. The Journal of Neuroscience, 35(40), 13698-13712.
24671605	1:1000	Wyatt, L.R., et al. 2014. Contribution of P2X4 receptors to ethanol intake in male C57BL/6 mice. Neurochemical research, 39(6), 1127-1139.
17070500	1:200	Kurano, Y., et al. 2006. . Chorein deficiency leads to upregulation of gephyrin and GABA_A receptor. Biochemical and biophysical research communications, 351(2), pp.438-442.

Western Blotting: Rat
PMID	Dilution	Publication
19144854	1:500	Rewal, M., et al. 2009. α-Containing GABA_A Receptors in the Nucleus Accumbens Mediate Moderate Intake of Alcohol. J. Neurosci., 29: 543 – 549.

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Anti-GABAA Receptor α1, N-Terminus Antibody

Our Anti-GABAA Receptor α1, N-Terminus primary antibody from PhosphoSolutions is rabbit polyclonal. It detects mouse and rat GABAA Receptor α1, N-Terminus and is antigen affinity purified. It is great for use in WB, IHC.