Name: Anti-GABA Transporter (GAT) 1 Antibody
Brand: PhosphoSolutions
SKU: 880-GAT1
Price: 415.0 USD
Availability: InStock

Our Anti-GABA Transporter (GAT) 1 primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, non-human primate, and rat GABA Transporter (GAT) 1 and is affinity purified. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Applications

IHC, WB

Host Species

Rabbit

Format

Affinity Purified

Gene Name

SLC6A1

Molecular Weight

67 kDa

Go to Full Product Details

Immunoflouresence of mouse retina specifically labeling GAT-1 protein (cat. 880-GAT1, red, 1:500) in the inner plexiform layer (IPL). The white identifies nuclei stained with DAPI. The retina was fixed with 4% paraformaldehyde for 30 minutes, embedded in 5% agarose, and sliced into 60 micron thick slices with a vibratome. The image is a Z-stack taken from a confocal microscope. Image courtesy of Andrea Wellington, Assistant Research Scientist, University of Arizona Physiology Dept.

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~67 kDa GAT-1 protein.

Volume: 100 µL

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$415

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Product Details

Target

GABA Transporter (GAT) 1

Target Description

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-1, the most abundant of the transporters, is found predominantly in neurons, but also in some specialized glia (Minelli et al., 1995). GAT-1 is thought to play a key role in epileptogenesis (Zhao et al. 2003).

Format

Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

SLC6A1

Molecular Weight

67 kDa

Antigen

Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat GAT1, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2492119

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:100-1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~67 kDa GAT-1 protein. Immunolabeling is blocked by pre-adsorption of antibody with the peptide used to generate the antibody.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

GABATHG antibody, GABATR antibody, GABT 1 antibody, GABT1 antibody, GAT-1 antibody, GAT1 antibody, SC6A1_HUMAN antibody, Slc6a1 antibody, Sodium and chloride dependent GABA transporter 1 antibody, Sodium- and chloride-dependent GABA transporter 1 antibody, Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody, Solute carrier family 6 member 1 antibody

UniProt Details

UniProt (Immunogen Species): P23978

NCBI Gene ID

79212

Product Specific References for Applications and Species

Immunohistochemistry: Human | Non-Human Primate| Rat | Salamanders

Immunohistochemistry: Human
PMID	Dilution	Publication
9623887	1:100	Conti, F., et al. 1998. Neuronal and glial localization of GAT‐1, a high‐affinity γ‐aminobutyric acid plasma membrane transporter, in human cerebral cortex: With a note on its distribution in monkey cortex. Journal of Comparative Neurology, 396(1), pp.51-63.

Immunohistochemistry: Non-Human Primate
PMID	Dilution	Publication
9623887	1:100	Conti, F., et al. 1998. Neuronal and glial localization of GAT‐1, a high‐affinity γ‐aminobutyric acid plasma membrane transporter, in human cerebral cortex: With a note on its distribution in monkey cortex. Journal of Comparative Neurology, 396(1), pp.51-63.

Immunohistochemistry: Rat
PMID	Dilution	Publication
8915826	not listed	Johnson, J., et al. 1997. Multiple γ-aminobutyric acid plasma membrane transporters (GAT-1, GAT-2, GAT-3) in the rat retina. The Journal of Comparative Neurology, 375(2), 212.

Immunohistochemistry: Salamander
PMID	Dilution	Publication
9390763	1:1000	Yang, C.Y., et al. 1997. Immunocytochemical localization of γ‐aminobutyric acid plasma membrane transporters in the tiger salamander retina. Journal of Comparative Neurology, 389(1), pp.117-126.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-GABA Transporter (GAT) 1 Antibody

Our Anti-GABA Transporter (GAT) 1 primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, non-human primate, and rat GABA Transporter (GAT) 1 and is affinity purified. It is great for use in WB, IHC.