Name: Anti-Fibrillarin Antibody
Brand: PhosphoSolutions
SKU: 560-FIB
Price: 360.0 USD
Availability: InStock

Our Anti-Fibrillarin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Fibrillarin and is Protein G purified. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Bovine, Chicken, Drosophila, Human, Mouse, Pig, Rat

Expected Reactivity

Canine, Feline, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Sheep, Vole

Clone

38F3

Applications

ICC, IHC, WB

Host Species

Mouse

Format

Protein G Purified

Gene Name

NOP1

Molecular Weight

34 kDa

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High magnification confocal image of HeLa cells showing strong, prominent labeling of the nucleoli in the nucleus with Anti-Fibrillarin (cat. 560-FIB, 1:100, green), colabeled with Anti-Vimentin (cat. 2105-VIM, red, 1:1000) showing the cytoplasmic intermediated filaments, and nuclear staining was done with DAPI (blue).

Immunofluorescence of human neuroblastoma SH-SY5Y cells showing strong, prominent labeling of the nucleoli in the nucleus with Anti-Fibrillarin (cat. 560-FIB, 1:500, green), and nuclear staining was done with DAPI (blue) which makes the fibrillarin marker appear pale blue.

Western blot of HeLa cell lysate showing specific immunolabeling of the ~34 kDa fibrillarin protein.

Volume: 100 µL

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Price:

$360

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Product Details

Target

Fibrillarin

Target Description

Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archaea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3). This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).

Format

Protein G Purified

Clonality

Monoclonal

Clone

38F3

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

NOP1

Molecular Weight

34 kDa

Antigen

Yeast nuclear preparation

Species Reactivity

Bovine, Chicken, Drosophila, Human, Mouse, Pig, Rat

Expected Reactivity

Canine, Feline, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Sheep, Vole

Antibody Registry ID

AB_2492093

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified culture supernatant.

Buffer

PBS + 50% glycerol and 5 mm NaN3

Dilution Ranges

WB: 1:1000
IHC: 1:100-1:500
ICC: 1:100-1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~34 kDa Fibrillarin /Nop1p protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

34 kD nucleolar scleroderma antigen antibody, 34 kDa nucleolar scleroderma antigen antibody, Fbl antibody, FBRL_HUMAN antibody, FIB antibody, FIB1 antibody, FLRN antibody, Histone-glutamine methyltransferase antibody, Nop1p antibody, RNA U3 small nucleolar interacting protein 1 antibody, RNU3IP1 antibody, rRNA 2' O methyltransferase fibrillarin antibody, rRNA 2'-O-methyltransferase fibrillarin antibody

UniProt Details

UniProt (Human): O00567
UniProt (Immunogen Species): P15646

NCBI Gene ID

851548

Product Specific Protocols

Western Blot Protocol: Download

General References

Paeschke, K., Simonsson, T., Postberg, J., Rhodes, D. and Lipps, H.J., 2005. Telomere end-binding proteins control the formation of G-quadruplex DNA structures in vivo. Nature Structural & Molecular Biology, 12(10), p.847. PMID: 16142245
Vermaak, D., Henikoff, S. and Malik, H.S., 2005. Positive selection drives the evolution of rhino, a member of the heterochromatin protein 1 family in Drosophila. PLoS Genetics, 1(1), 96-108. PMID: 16103923
Tyagi, S. and Alsmadi, O., 2004. Imaging native β-actin mRNA in motile fibroblasts. Biophysical Journal, 87(6), pp.4153-4162. PMID: 15377515
Newton, K., Petfalski, E., Tollervey, D. and Cáceres, J.F., 2003. Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse. Molecular and Cellular Biology, 23(23), pp.8519-8527. PMID: 14612397
Aris, J.P. and Blobel, G., 1988. Identification and characterization of a yeast nucleolar protein that is similar to a rat liver nucleolar protein. The Journal of Cell Biology, 107(1), pp.17-31. PMID: 3292539
Ochs, R.L., Lischwe, M.A., Spohn, W.H. and Busch, H., 1985. Fibrillarin: a new protein of the nucleolus identified by autoimmune sera. Biology of the Cell, 54(2), pp.123-133. PMID: 2933102

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Anti-Fibrillarin Antibody (38F3)

Our Anti-Fibrillarin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Fibrillarin and is Protein G purified. It is great for use in WB, IHC, ICC.

Anti-Fibrillarin Antibody
SKU: 560-FIB

Bulk Order Anti-Fibrillarin Antibody

Product Details

Product Specific Protocols

General References

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Anti-Fibrillarin Antibody (38F3)

Our Anti-Fibrillarin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Fibrillarin and is Protein G purified. It is great for use in WB, IHC, ICC.

Anti-Fibrillarin Antibody SKU: 560-FIB

Bulk Order Anti-Fibrillarin Antibody

Product Details

Product Specific Protocols

General References

Anti-Fibrillarin Antibody
SKU: 560-FIB